Improving the catalytic activity of hyperthermophilic Pyrococcus prolidases for detoxification of organophosphorus nerve agents over a broad range of temperatures

Applied Microbiology and Biotechnology

Volumn 87, Issue 5, 2010, Pages 1715-1726

  Theriot, Casey M ^a   Du, Xuelian ^a   Tove, Sherry R ^a   Grunden, Amy M ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Directed evolution; Mutagenesis; OP nerve agents; Prolidase; Pyrococcus furiosus

Indexed keywords

BROAD TEMPERATURE RANGES; CATALYTIC ACTIVITY; CHEMICAL WARFARE AGENTS; DI-PEPTIDES; DIRECTED EVOLUTION; ENZYME STABILITY; ERROR PRONE PCR; HYPERTHERMOPHILIC; HYPERTHERMOPHILIC ARCHAEON; NERVE AGENTS; ORGANOPHOSPHORUS COMPOUNDS; POSITIVE SELECTION; PYROCOCCUS FURIOSUS; STRUCTURAL FACTOR; THERMOACTIVITY;

CHEMICAL WARFARE; DECONTAMINATION; DETOXIFICATION; ENZYMES; ESCHERICHIA COLI; PHOSPHORUS COMPOUNDS;

MUTAGENESIS;

ORGANOPHOSPHORUS COMPOUND; PROLINE; PROLINE DIPEPTIDASE; ARCHAEAL PROTEIN; CHEMICAL WARFARE AGENT; CULTURE MEDIUM; DIPEPTIDASE; PESTICIDE;

AMINO ACID; BACTERIUM; CATALYSIS; DETOXIFICATION; ENZYME ACTIVITY; EVOLUTIONARY BIOLOGY; GENE EXPRESSION; MICROBIAL ACTIVITY; MUTATION; ORGANOPHOSPHATE; PEPTIDE; PESTICIDE; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN; TEMPERATURE EFFECT;

ARTICLE; BACTERIAL STRAIN; CATALYSIS; DETOXIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PYROCOCCUS; AMINO ACID SUBSTITUTION; CHEMICAL STRUCTURE; CHEMISTRY; CULTURE MEDIUM; ENZYMOLOGY; GENE DELETION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PLASMID; PROCEDURES; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; PYROCOCCUS FURIOSUS; TEMPERATURE;

AMINO ACID SUBSTITUTION; ARCHAEAL PROTEINS; CHEMICAL WARFARE AGENTS; CULTURE MEDIA; DIPEPTIDASES; ENZYME STABILITY; ESCHERICHIA COLI; MODELS, MOLECULAR; MUTAGENESIS; ORGANOPHOSPHORUS COMPOUNDS; PESTICIDES; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; PYROCOCCUS FURIOSUS; SEQUENCE DELETION; TEMPERATURE;

ARCHAEA; ESCHERICHIA COLI; PYROCOCCUS; PYROCOCCUS FURIOSUS;

EID: 77955521279     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2614-3     Document Type: Article

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