Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: Insights into substrate recognition and the mechanism of catalysis

Biochemistry

Volumn 45, Issue 3, 2006, Pages 964-975

  Graham, Stephen C ^a   Lilley, Penelope E ^b   Lee, Mihwa ^a   Schaeffer, Patrick M ^b   Kralicek, Andrew V ^b,c   Dixon, Nicholas E ^b   Guss, J Mitchell ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^c THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CRYSTALLOGRAPHY; ESCHERICHIA COLI; HYDROPHOBICITY; MUTAGENESIS; OXYGEN; POLYPEPTIDES;

ACTIVE SITE; METAL LIGANDS; PROLINE-BINDING POCKET; SUBSTRATE BINDING;

ENZYMES;

AMINOPEPTIDASE P; CARBONYL DERIVATIVE; HISTIDINE; LIGAND; MANGANESE; METAL; METHIONYL AMINOPEPTIDASE; MUTANT PROTEIN; N METHYLALANINE; OXYGEN; POLYPEPTIDE; PROLINE; PROLINE DIPEPTIDASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENETIC CONSERVATION; HYDROPHOBICITY; MOLECULAR RECOGNITION; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FOLDING; X RAY CRYSTALLOGRAPHY;

AMINOPEPTIDASES; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI;

EID: 31044431736     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0518904     Document Type: Article

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