Simultaneous detection of cow and buffalo milk in mozzarella cheese by Real-Time PCR assay

Food Chemistry

Volumn 124, Issue 1, 2011, Pages 362-366

  Dalmasso, Alessandra ^a   Civera, Tiziana ^a   La Neve, Fabio ^a   Bottero, Maria Teresa ^a  

^a UNIVERSITY OF TURIN   (Italy)

Author keywords

Mozzarella cheese; Real Time PCR Allelic Discrimination; Species identification

Indexed keywords

BOS TAURUS; BUFFALO MILK; CONSERVED REGIONS; COW MILK; DETECTION THRESHOLD; MILK SAMPLE; MITOCHONDRIAL CYTOCHROME B; MOZZARELLA CHEESE; ONE STEP; REAL-TIME PCR; REAL-TIME PCR ALLELIC DISCRIMINATION; REAL-TIME PCR ASSAY; RETAIL TRADE; SIMULTANEOUS DETECTION; SPECIES IDENTIFICATION; SPECIFIC PRIMERS;

DAIRY PRODUCTS; GENES; PROBES; SALES;

POLYMERASE CHAIN REACTION;

CYTOCHROME B;

ARTICLE; BOS TAURUS; BUBALUS BUBALIS; BUFFALO; BUFFALO MILK; CHEESE; COW; DNA EXTRACTION; MILK; MOZZARELLA CHEESE; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SPECIES IDENTIFICATION;

BOS TAURUS; BUBALUS BUBALIS;

EID: 77955277013     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodchem.2010.06.017     Document Type: Article

References (30)

1. Bottero M.T., Civera T., Anastasio A., Turi R.M., Rosati S. Identification of cow's milk in " buffalo" cheese by duplex polymerase chain reaction. Journal of Food Protection 2002, 65:362-366.
2. Bottero M.T., Civera T., Nucera D., Rosati S., Sacchi P., Turi R.M. A multiplex polymerase chain reaction for the identification of cows', goats' and sheep's milk in dairy products. International Dairy Journal 2003, 13:277-282.
3. Bottero M.T., Dalmasso A., Cappelletti M., Secchi C., Civera T. Differentiation of five tuna species by a multiplex primer-extension assay. Journal of Biotechnology 2007, 129:575-580.
4. Branciari R., Nijman I.J., Plas M.E., Di Antonio E., Lenstra J.A. Species origin of milk in Italian mozzarella and Greek feta cheese. Journal of Food Protection 2000, 63:408-411.
5. Campsall P.A., Au N.H.C., Prendiville J.S., Speert D.P., Tan R., Thomas E.E. Detection and genotyping of varicella-zoster virus by TaqMan Allelic Discrimination Real-Time PCR. Journal of Clinical Microbiology 2004, 42:1409-1413.
6. Chen R.K., Chang L.W., Chung Y.Y., Lee M.H., Ling Y.C. Quantification of cow milk adulteration in goat milk using high-performance liquid chromatography with electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry 2004, 18:1167-1171.
7. Cozzolino R., Passalacqua S., Salemi S., Garozzo D. Identification of adulteration in water buffalo mozzarella and in ewe cheese by using whey proteins as biomarkers and matrix-assisted laser desorption/ionization mass spectrometry. Journal of Mass Spectrometry 2002, 37:985-991.
8. De La Fuente M.A., Juárez M. Authenticity assessment of dairy products. Critical Reviews in Food Science and Nutrition 2005, 45:563-585.
9. EC Regulation No. 1107/96 (1996). Commission Regulation (EC) No. 1107/96 of 12 June 1996 on the registration of geographical indications and designations of origin under the procedure laid down in Article 17 of Council Regulation (EEC) No. 2081/92. Official Journal of the European Commission, June 21, 1996, 1-15.
10. EC Regulation No. 213/2001 (2001). Commission Regulation (EC) No. 213/2001 of the Commission of 9 January Annex XV, Reference method for the detection of cows' milk and caseinate in cheeses from ewes' milk, goats' milk or buffalos' milk or mixtures of ewes' and buffalos' milk. Official Journal of the European Commission, February 7, 2001, 1-99.
11. El-Rady A., Sayed M. Identification of milk source by polymerase chain reaction-restriction fragment length polymorphism analysis. Journal of Rapid Methods and Automation in Microbiology 2006, 14:146-155.
12. Higgins D.G., Bleasby A.J., Fuchs R. CLUSTAL V: Improved software for multiple sequence alignment. Computer Applications in Biosciences 1992, 8:189-191.
13. Hurley I.P., Ireland H.E., Coleman R.C., Williams J.H.H. Application of immunological methods for the detection of species adulteration in dairy products. International Journal of Food Science and Technology 2004, 39:873-878.
14. La Neve F., Civera T., Mucci N., Bottero M.T. Authentication of meat from game and domestic species by SNaPshot minisequencing analysis. Meat Science 2007, 10.1016/j.meatsci.2007.11.027.
15. Lockley A.K., Bardsley R.G. DNA-based methods for food authentication. Trends in Food Science & Technology 2000, 11:67-77.
16. López-Calleja I., Alonso G., Fajardo V., Rodriguez M.A., Hernández P.E., García T., et al. PCR detection of cows' milk in water buffalo milk and mozzarella cheese. International Dairy Journal 2005, 15:1122-1129.
17. López-Calleja I.M., González I., Fajardo V., Hernández P.E., García T., Martín R. Application of an indirect ELISA and a PCR technique for detection of cows' milk in sheep's and goats' milk cheeses. International Dairy Journal 2007, 17:87-93.
18. López-Calleja I., González I., Fajardo V., Martín I., Hernández P.E., García T., et al. Quantitative detection of goats' milk in sheep's milk by real-time PCR. Food Control 2007, 18:1466-1473.
19. López-Calleja I., González I., Fajardo V., Martín I., Hernández P.E., García T., et al. Real-time TaqMan PCR for quantitative detection of cows' milk in ewes' milk mixtures. International Dairy Journal 2007, 17:729-736.
20. Lopparelli R.M., Cardazzo B., Balzan S., Giaccone V., Novelli E. Real-Time TaqMan Polymerase chain reaction detection and quantification of cow DNA in pure water buffalo mozzarella cheese: Method validation and its application on commercial samples. Journal of Agricultural and Food Chemistry 2007, 55:3429-3434.
21. Mafra I., Roxo Á., Ferreira I.M., Oliveira M. A duplex polymerase chain reaction for the quantitative detection of cows' milk in goats' milk cheese. International Dairy Journal 2007, 17:1132-1138.
22. Mayer H.K. Milk species identification in cheese varieties using electrophoretic, chromatographic and PCR techniques. International Dairy Journal 2005, 15:595-604.
23. Mirabaud S., Rolando C., Regert M. Molecular criteria for discriminating adipose fat and milk from different species by NanoESI MS and MS/MS of their triacylglycerols: Application to archaeological remains. Analytical Chemistry 2007, 79:6182-6192.
24. Partis L., Croan D., Guo Z., Clark R., Coldham T., Murby J. Evaluation of a DNA fingerprinting method for determining the species origin of meats. Meat Science 2000, 54:369-376.
25. Quinteiro J., Sotelo C.G., Rehbein H., Pryde S.E., Medina I., Pérez-Martín R.I., et al. Use of mtDNA direct polymerase chain reaction (PCR) sequencing and PCR-restriction fragment length polymorphism methodologies in species identification of canned tuna. Journal of Agricultural and Food Chemistry 1998, 46:1662-1669.
26. Rea S., Chikuni K., Branciari R., Sangamayya R., Ranucci D., Avellini P. Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese. Journal of Dairy Research 2001, 68:689-698.
27. Stopińska K., Grzybowski T., Malyarchuk B.A., Derenko M.V., Miścicka-Śliwka D. Optimization of the Y831C mutation detection in human DNA polymerase gamma by allelic discrimination assay. Acta Biochimica Polonica 2006, 53:591-595.
28. U'Ren J.M., Van Ert M.N., Schupp J.M., Easterday W.R., Simonson T.S., Okinaka R.T., et al. Use of a Real-Time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei. Journal of Clinical Microbiology 2005, 43:5771-5774.
29. Woolfe M., Primrose S. Food forensics: Using DNA technology to combat misdescription and fraud. Trends in Biotechnology 2004, 22:222-226.
30. Zhang C.L., Fowler M.R., Scott N.W., Lawson G., Slater A. A TaqMan real-time PCR system for the identification and quantification of bovine DNA in meats, milks and cheeses. Food Control 2007, 18:1149-1158.