Heterologous expression and purification of a biologically active legume lectin from Cratylia mollis seeds (CRAMOLL 1)

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1804, Issue 9, 2010, Pages 1917-1924

  Varejão, Nathalia ^a   Almeida, Marcius da Silva ^a   De Cicco, Nuccia N T ^a   Atella, Geórgia C ^a   Coelho, Luana C B B ^b   Correia, Ma Tereza S ^b   Foguel, Debora ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

Author keywords

Cratylia mollis seed lectin; Gene synthesis; Heterologous expression; Legume lectins; Protein stability

Indexed keywords

CRAMOLL 1; DNA; LECTIN; MONOSACCHARIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; PLANT LECTIN;

ACIDIFICATION; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL CELL; ANTIGEN ANTIBODY REACTION; ARTICLE; CELL INCLUSION; CONTROLLED STUDY; COTYLEDON; CRATYLIA MOLLIS; EPIMASTIGOTE; ERYTHROCYTE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CONTROL; HEMAGGLUTINATION; HETEROLOGOUS EXPRESSION; HIGH TEMPERATURE; HYDROSTATIC PRESSURE; LEGUME; MOLECULAR WEIGHT; NONHUMAN; OLIGOMERIZATION; PH; PLANT SEED; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; THERMOSTABILITY; TRYPANOSOMA CRUZI; ANIMAL; CHEMISTRY; CIRCULAR DICHROISM; FABACEAE; GENETICS; HEMAGGLUTINATION TEST; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR CLONING; RABBIT;

BACTERIA (MICROORGANISMS); CRATYLIA MOLLIS; ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS; TRYPANOSOMA CRUZI;

ANIMALS; CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; FABACEAE; HEMAGGLUTINATION TESTS; PLANT LECTINS; RABBITS; RECOMBINANT PROTEINS; SEEDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPANOSOMA CRUZI;

EID: 77955093115     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2010.06.004     Document Type: Article

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