SUMO fusion facilitates expression and purification of garlic leaf lectin but modifies some of its properties

Journal of Biotechnology

Volumn 146, Issue 1-2, 2010, Pages 1-8

  Upadhyay, Santosh Kumar ^a   Saurabh, Sharad ^a   Rai, Preeti ^a   Singh, Rahul ^a   Chandrashekar, K ^a   Verma, Praveen C ^a   Singh, P K ^a   Tuli, Rakesh ^a  

^a CSIR NATIONAL BOTANICAL RESEARCH INSTITUTE   (India)

Author keywords

ASAL; Glycan array; Haemagglutination; Insecticidal protein; Lectin; Sugar specificity; SUMO

Indexed keywords

ALLIUM SATIVUM; ARRAY ANALYSIS; ASAL; CARBOHYDRATE BINDING; E. COLI; FUSION PROTEINS; HOMODIMERS; IMMOBILIZED METAL AFFINITY COLUMNS; INCLUSION BODIES; INSECTICIDAL PROTEIN; N-TERMINALS; OVER-EXPRESSION; SMALL UBIQUITIN-RELATED MODIFIERS; STERIC HINDRANCES; TRANSGENIC PLANTS;

CARBOHYDRATES; CHEMICAL REACTIONS; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; ESCHERICHIA COLI; METAL REFINING; PEPTIDES; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; SUGAR (SUCROSE);

GENE EXPRESSION;

HOMODIMER; HYBRID PROTEIN; PLANT LECTIN; SUMO PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOLOGICAL PEST CONTROL; CHEMICAL STRUCTURE; CONTROLLED STUDY; DIMERIZATION; ESCHERICHIA COLI; GARLIC; GEL PERMEATION CHROMATOGRAPHY; HEMAGGLUTINATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INSECTICIDAL ACTIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT BIOTECHNOLOGY; PLANT LEAF; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; TRANSGENIC PLANT;

AGGLUTINATION; ANIMALS; CYSTEINE ENDOPEPTIDASES; DISK DIFFUSION ANTIMICROBIAL TESTS; DNA, PLANT; GARLIC; HYDROGEN-ION CONCENTRATION; LARVA; MANNOSE-BINDING LECTINS; MICROARRAY ANALYSIS; PLANT LEAVES; PLANT PROTEINS; POLYSACCHARIDES; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; SPODOPTERA; SUMO-1 PROTEIN; TEMPERATURE;

ALLIUM SATIVUM;

EID: 77249104438     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.01.013     Document Type: Article

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