High-throughput protein concentration and buffer exchange: Comparison of ultrafiltration and ammonium sulfate precipitation

Methods in Molecular Biology

Volumn 498, Issue , 2009, Pages 309-314

  Moore, Priscilla A ^a   Kery, Vladimir ^b  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b Biomimetic Therapeutics   (United States)

Author keywords

Ammonium sulfate precipitation; Buffer exchange; Desaltation; High throughput; Protein concentration; Ultrafiltration

Indexed keywords

SHEWANELLA ONEIDENSIS;

EID: 84934435325     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-196-3_20     Document Type: Article

References (3)

1. van Reis, R., and Zydney, A. (2001) Membrane separations in biotechnology. Curr Opin Biotechnol 12(2), 208-211.
2. Markillie, L. M., Lin, J. T., Adkins, J. N., Auberry, D. L., Hill, E. A., Hooker, B. S., Moore, P. A., Moore, R. J., Shi, L., Wiley, H. S., and Kery, V. (2005) Simple protein complex purification and identification method for high-throughput mapping of protein interaction networks. J Proteome Res 4(2), 268-274.
3. Lin, C. T., Moore, P. A., Auberry, D. L., Landorf, E. V., Peppler, T., Victry, K. D., Collart, F. R., and Kery, V. (2006) Automated purification of recombinant proteins: combining high-throughput with high yield. Protein Expr Purif 47(1), 16-24.