Access to gram scale amounts of functional globular adiponectin from E. coli inclusion bodies by alkaline-shock solubilization

Biochemical and Biophysical Research Communications

Volumn 398, Issue 1, 2010, Pages 32-37

  Heiker, John T ^a   Klöting, Nora ^a   Blüher, Matthias ^a   Beck Sickinger, Annette G ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Adiponectin; Alkaline shock; Inclusion bodies; Obesity; Recombinant protein expression

Indexed keywords

ADIPONECTIN; ALKALI;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIOLOGICAL ACTIVITY; CELL INCLUSION; CELL STRAIN MCF 7; CONTROLLED STUDY; DRUG EFFECT; DRUG EFFICACY; DRUG MANUFACTURE; ESCHERICHIA COLI; INSULIN DEPENDENT DIABETES MELLITUS; MALE; MOUSE; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; SOLUBILIZATION;

ADIPONECTIN; ALKALIES; ANIMALS; BLOOD GLUCOSE; CELL LINE, TUMOR; DIABETES MELLITUS, EXPERIMENTAL; DIABETES MELLITUS, TYPE 1; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; MICE; RECOMBINANT PROTEINS; SOLUBILITY; UREA;

MUS;

EID: 77954756849     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.06.020     Document Type: Article

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