Imaging living synapses at the nanoscale by STED microscopy

Journal of Neuroscience

Volumn 30, Issue 28, 2010, Pages 9341-9346

  Nägerl, U Valentin ^a   Bonhoeffer, Tobias ^b  

^a INSTITUT FRANÇOIS MAGENDIE   (France)

^b MAX PLANCK INSTITUTE OF NEUROBIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CONFOCAL MICROSCOPY; DENDRITIC SPINE; ELECTRON MICROSCOPY; HIPPOCAMPUS; IMAGE ANALYSIS; MICROSCOPY; NANOANALYSIS; NONHUMAN; PRIORITY JOURNAL; REVIEW; STIMULATED EMISSION DEPLETION MICROSCOPY; SYNAPSE;

ANIMALS; MICROSCOPY, FLUORESCENCE; NEURONS; SOFTWARE; SYNAPSES;

EID: 77954723624     PISSN: 02706474     EISSN: 15292401     Source Type: Journal    
DOI: 10.1523/JNEUROSCI.0990-10.2010     Document Type: Review

References (27)

1. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF (2006) Imaging intracellular fluorescent proteins atnanometer resolution. Science 313:1642-1645.
2. Ding JB, Takasaki KT, Sabatini BL (2009) Supraresolution imaging in brain slices using stimulated-emission depletion two-photon laser scanning microscopy. Neuron 63:429-437.
3. Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, Wallace M, Nerbonne JM, Lichtman JW, Sanes JR (2000) Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28:41-51.
4. Gähwiler BH (1981) Organotypic monolayer cultures of nervous tissue. J Neurosci Methods 4:329-342.
5. Gustafsson MG (2000) Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc 198:82-87.
6. Gustafsson MG (2005) Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. Proc Natl Acad Sci U S A 102:13081-13086.
7. Harke B, Keller J, Ullal CK, Westphal V, Schönle A, Hell SW (2008) Resolution scaling in STED microscopy. Opt Express 16:4154-4162.
8. Harris KM, Kater SB (1994) Dendritic spines: cellular specializations imparting both stability and flexibility to synaptic function. Annu Rev Neurosci 17:341-371.
9. Hein B, Willig KI, Hell SW (2008) Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell. Proc Natl Acad Sci U S A 105:14271-14276.
10. Hell SW (2003) Toward fluorescence nanoscopy. Nat Biotechnol 21:1347-1355.
11. Hell SW (2009) Microscopy and its focal switch. Nat Methods 6:24-32.
12. Hell SW, Wichmann J (1994) Breaking the diffraction resolution limit by stimulated emission depletion fluorescence microscopy. Optics Lett 19:780-782.
13. Hess ST, Girirajan TP, Mason MD (2006) Ultrahigh resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91:4258-4272.
14. Huang B, Wang W, Bates M, Zhuang X (2008) Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science 319:810-813. (Pubitemid 351225870)
15. Kittel RJ, Wichmann C, Rasse TM, Fouquet W, Schmidt M, Schmid A, Wagh DA, Pawlu C, Kellner RR, Willig KI, Hell SW, Buchner E, Heckmann M, Sigrist SJ (2006) Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release. Science 312:1051-1054.
16. Li Q, Wu SS, Chou KC (2009) Subdiffractionlimit two-photon fluorescence microscopy for GFP-tagged cell imaging. Biophys J 97:3224-3228.
17. Nägerl UV, Willig KI, Hein B, Hell SW, Bonhoeffer T (2008) Live-cell imaging of dendritic spines by STED microscopy. Proc Natl Acad Sci U S A 105:18982-18987.
18. Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, Bradke F, Jenne D, Holak TA, Werb Z, Sixt M, Wedlich-Soldner R (2008) Lifeact: a versatile marker to visualize F-actin. Nat Methods 5:605-607. (Pubitemid 351911864)
19. Rust MJ, Bates M, Zhuang X (2006) Subdiffraction- limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3:793-795. (Pubitemid 44445825)
20. Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MG, Leonhardt H, Sedat JW (2008) Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science 320:1332-1336.
21. Sieber JJ, Willig KI, Heintzmann R, Hell SW, Lang T (2006) The SNARE motif is essential for the formation of syntaxin clusters in the plasma membrane. Biophys J 90:2843-2851.
22. Vaziri A, Tang J, Shroff H, Shank CV (2008) Multilayer three-dimensional super resolution imaging of thick biological samples. Proc Natl Acad Sci U S A 105:20221-20226.
23. Westphal V, Rizzoli SO, Lauterbach MA, Kamin D, Jahn R, Hell SW (2008) Video-rate farfield optical nanoscopy dissects synaptic vesicle movement. Science 320:246-249. (Pubitemid 351521423)
24. Wildanger D, Rittweger E, Kastrup L, Hell SW (2008) STED microscopy with a supercontinuum laser source. Opt Express 16:9614-9621.
25. Wildanger D, Bückers J, Westphal V, Hell SW, Kastrup L (2009) A STED microscope aligned by design. Opt Express 17:16100-16110.
26. Willig KI, Rizzoli SO, Westphal V, Jahn R, Hell SW (2006) STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 440:935-939.
27. Wolter S, Schüttpelz M, Tscherepanow M, van de Linde S, Heilemann M, Sauer M (2010) Real-time computation of subdiffractionresolution fluorescence images. J Microsc 237: 12-22.