The human male reproductive tract antimicrobial peptides of the HE2 family exhibit potent synergy with standard antibiotics

Journal of Peptide Science

Volumn 16, Issue 7, 2010, Pages 337-341

  Yenugu, Suresh ^a   Narmadha, Ganapathy ^a  

^a UNIVERSITY OF HYDERABAD   (India)

Author keywords

Antimicrobial; Epididymis; Fractional inhibitory concentration index; Synergy

Indexed keywords

AMPICILLIN; CARBENICILLIN; CHLORAMPHENICOL; CIPROFLOXACIN; DOXYCYCLINE; GENTAMICIN; KANAMYCIN; POLYPEPTIDE ANTIBIOTIC AGENT; PROTEIN HE2ALPHA; PROTEIN HE2BETA 2; RIFAMPICIN; STREPTOMYCIN; TETRACYCLINE; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; GLYCOPEPTIDE; MEMBRANE ANTIGEN; SPAG11A PROTEIN, HUMAN;

AMINO ACID SEQUENCE; ANTIBACTERIAL ACTIVITY; ARTICLE; CONTROLLED STUDY; DRUG POTENTIATION; EPIDIDYMIS; ESCHERICHIA COLI; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; DRUG EFFECTS; HUMAN; IMMUNOLOGY; INNATE IMMUNITY; MALE; MALE GENITAL SYSTEM; MICROBIAL SENSITIVITY TEST;

ESCHERICHIA COLI;

ANTI-BACTERIAL AGENTS; ANTIGENS, SURFACE; DRUG SYNERGISM; ESCHERICHIA COLI; GENITALIA, MALE; GLYCOPEPTIDES; HUMANS; IMMUNITY, INNATE; MALE; MICROBIAL SENSITIVITY TESTS;

EID: 77954713961     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.1246     Document Type: Article

References (48)

1. Ganz T. Antimicrobial polypeptides. J. Leukoc. Biol. 2004; 75: 34-38.
2. Bastian A, Schafer H. Human alpha-defensin 1 (HNP-1) inhibits adenoviral infection in vitro. Regul. Pept. 2001; 101: 157-161. (Pubitemid 32728382)
3. Daher KA, Selsted ME, Lehrer RI. Direct inactivation of viruses by human granulocyte defensins. J. Virol. 1986; 60: 1068-1074. (Pubitemid 17179284)
4. Schibli DJ, Hunter HN, Aseyev V, Starner TD, Wiencek JM, McCray PB Jr, Tack BF, Vogel HJ. The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus. J. Biol. Chem. 2002; 277: 8279-8289.
5. Lehrer RI, Ganz T. Defensins of vertebrate animals. Curr. Opin. Immunol. 2002; 14: 96-102.
6. Zasloff M. Antimicrobial peptides of multicellular organisms. Nature 2002; 415: 389-395.
7. Matsuzaki K. Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim. Biophys. Acta 1998; 1376: 391-400.
8. Heller WT, Waring AJ, Lehrer RI, Harroun TA, Weiss TM, Yang L, Huang HW. Membrane thinning effect of the beta-sheet antimicrobial protegrin. Biochemistry Mosc. 2000; 39: 139-145. (Pubitemid 30033328)
9. Gazit E, Boman A, Boman HG, Shai Y. Interaction of the mammalian antibacterial peptide cecropin P1 with phospholipid vesicles. Biochemistry Mosc. 1995; 34: 11 479-11 488.
10. Park CB, Kim HS, Kim SC. Mechanism of action of the antimicrobial peptide buforin II: buforin II kills microorganisms by penetrating the cell membrane and inhibiting cellular functions. Biochem. Biophys. Res. Commun. 1998; 244: 253-257.
11. Subbalakshmi C, Sitaram N. Mechanism of antimicrobial action of indolicidin. FEMS Microbiol. Lett. 1998; 160: 91-96.
12. Boman HG, Agerberth B, Boman A. Mechanisms of action on Escherichia coli of cecropin P1 and PR-39, two antibacterial peptides from pig intestine. Infect. Immun. 1993; 61: 2978-2984. (Pubitemid 23182296)
13. Otvos L Jr, O I, Rogers ME, Consolvo PJ, Condie BA, Lovas S, Bulet P, Blaszczyk-Thurin M. Interaction between heat shock proteins and antimicrobial peptides. Biochemistry Mosc. 2000; 39: 14 150-14 159.
14. LehrerRI, BartonA, DaherKA, HarwigSS, GanzT, SelstedME. Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity. J. Clin. Invest. 1989; 84: 553-561. (Pubitemid 19196987)
15. Hall SH, Hamil KG, French FS. Host defense proteins of the male reproductive tract. J. Androl. 2002; 23: 585-597.
16. Malm J, Sorensen O, Persson T, Frohm-Nilsson M, Johansson B, Bjartell A, Lilja H, Stahle-Backdahl M, Borregaard N, Egesten A. The human cationic antimicrobial protein (hCAP-18) is expressed in the epithelium of human epididymis, is present in seminal plasma at high concentrations, and is attached to spermatozoa. Infect. Immun. 2000; 68: 4297-4302.
17. Palladino MA, Mallonga TA, Mishra MS. Messenger RNA (mRNA) expression for the antimicrobial peptides beta-defensin-1 and beta-defensin-2 in the male rat reproductive tract: beta-defensin-1 mRNA in initial segment and caput epididymidis is regulated by androgens and not bacterial lipopolysaccharides. Biol. Reprod. 2003; 68: 509-515.
18. Zaballos A, Villares R, Albar JP, Martinez AC, Marquez G. Identification onmouse chromosome 8 of new beta-defensin genes with regionally specific expression in the male reproductive organ. J. Biol. Chem. 2004; 279: 12 421-12 426.
19. Yamaguchi Y, Nagase T, Makita R, Fukuhara S, Tomita T, Tominaga T, Kurihara H, Ouchi Y. Identification of multiple novel epididymisspecific beta-defensin isoforms in humans and mice. J. Immunol. 2002; 169: 2516-2523. (Pubitemid 34920996)
20. Com E, Bourgeon F, Evrard B, Ganz T, Colleu D, Jegou B, Pineau C. Expression of antimicrobial defensins in themale reproductive tract of rats,mice, and humans. Biol. Reprod. 2003; 68: 95-104. (Pubitemid 36076323)
21. Li P, Chan HC, He B, So SC, Chung YW, Shang Q, Zhang YD, Zhang YL. An antimicrobial peptide gene found in the male reproductive system of rats. Science 2001; 291: 1783-1785. (Pubitemid 32198982)
22. Hamil KG, Liu Q, Sivashanmugam P, Yenugu S, Soundararajan R, Grossman G, Richardson RT, Zhang YL, O'Rand MG, Petrusz P, French FS, Hall SH. Cystatin 11: a new member of the cystatin type 2 family. Endocrinology 2002; 143: 2787-2796.
23. Blankenvoorde MF, van't Hof W, Walgreen-Weterings E, van Steenbergen TJ, Brand HS, Veerman EC, Nieuw Amerongen AV. Cystatin and cystatin-derived peptides have antibacterial activity against the pathogen Porphyromonas gingivalis. Biol. Chem. 1998; 379: 1371-1375.
24. Jin YZ, Bannai S, Dacheux F, Dacheux JL, Okamura N. Direct evidence for the secretion of lactoferrin and its binding to sperm in the porcine epididymis. Mol. Reprod. Dev. 1997; 47: 490-496. (Pubitemid 27273160)
25. Reddy ES, Bhargava PM. Seminalplasmin - an antimicrobial protein frombovine seminalplasmawhich acts in E. coli by specific inhibition of rRNA synthesis. Nature 1979; 279: 725-728.
26. Bourgeon F, Evrard B, Brillard-Bourdet M, Colleu D, Jegou B, Pineau C. Involvement of semenogelin-derived peptides in the antibacterial activity of human seminal plasma. Biol. Reprod. 2004; 70: 768-774. (Pubitemid 38233717)
27. Hamil KG, Sivashanmugam P, Richardson RT, Grossman G, Ruben SM, Mohler JL, Petrusz P, O'Rand MG, French FS, Hall SH. HE2beta and HE2gamma, new members of an epididymis-specific family of androgen-regulated proteins in the human. Endocrinology 2000; 141: 1245-1253.
28. von Horsten HH, Derr P, Kirchhoff C. Novel antimicrobial peptide of human epididymal duct origin. Biol. Reprod. 2002; 67: 804-813. (Pubitemid 34925198)
29. Liu Q, Hamil KG, Sivashanmugam P, Grossman G, Soundararajan R, Rao AJ, Richardson RT, Zhang YL, O'Rand MG, Petrusz P, French FS, Hall SH. Primate epididymis-specific proteins: characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human. Endocrinology 2001; 142: 4529-4539.
30. Yenugu S, Hamil KG, Birse CE, Ruben SM, French FS, Hall SH. Antibacterial properties of the sperm-binding proteins and peptides of human epididymis 2 (HE2) family; salt sensitivity, structural dependence and their interaction with outer and cytoplasmic membranes of Escherichia coli. Biochem. J. 2003; 372: 473-483. (Pubitemid 36723893)
31. Liao M, Ruddock PS, Rizvi AS, Hall SH, French FS, Dillon JR. Cationic peptide of the male reproductive tract, HE2{alpha}, displays antimicrobial activity against Neisseria gonorrhoeae, Staphylococcus aureus and Enterococcus faecalis. J. Antimicrob. Chemother. 2005; 56: 957-961. (Pubitemid 41631927)
32. Yenugu S, Hamil KG, French FS, Hall SH. Antimicrobial actions of the human epididymis 2 (HE2) protein isoforms, HE2alpha, HE2beta1 and HE2beta2. Reprod. Biol. Endocrinol. 2004; 2: 61.
33. Yenugu S, Hamil KG, French FS, Hall SH. Antimicrobial actions of human and macaque sperm associated antigen (SPAG)11 isoforms: influence of the N-terminal peptide. Mol. Cell. Biochem. 2006; 284: 25-35.
34. Yenugu S, Hamil KG, Radhakrishnan Y, French FS, Hall SH. The androgen-regulated epididymal sperm-binding protein, human beta-defensin 118 (DEFB118) (formerly ESC42), is an antimicrobial beta-defensin. Endocrinology 2004; 145: 3165-3173. (Pubitemid 38829987)
35. Trojian TH, Lishnak TS, Heiman D. Epididymitis and orchitis: an overview. Am. Fam. Physician 2009; 79: 583-587.
36. Fidai S, Farmer SW, Hancock RE. Interaction of cationic peptides with bacterialmembranes. In Antibacterial Peptide Protocols, Vol. 78, Shafer W(ed.). Humana Press: New Jersey, 1997; 187-204.
37. Duggineni S, Srivastava G, Kundu B, Kumar M, Chaturvedi AK, Shukla PK. A novel dodecapeptide from a combinatorial synthetic library exhibits potent antifungal activity and synergy with standard antimycotic agents. Int. J. Antimicrob. Agents 2007; 29: 73-78. (Pubitemid 44959784)
38. Shin SY, Yang ST, Park EJ, Eom SH, Song WK, Kim Y, Hahm KS, Kim JI. Salt resistance and synergistic effect with vancomycin of alphahelical antimicrobial peptide P18. Biochem. Biophys. Res. Commun. 2002; 290: 558-562.
39. Lawyer C, Pai S, Watabe M, Bakir H, Eagleton L, Watabe K. Effects of synthetic form of tracheal antimicrobial peptide on respiratory pathogens. J. Antimicrob. Chemother. 1996; 37: 599-604. (Pubitemid 26119579)
40. Piers KL, Brown MH, Hancock RE. Improvement of outer membrane- permeabilizing and lipopolysaccharide-binding activities of an antimicrobial cationic peptide by C-terminal modification. Antimicrob. Agents Chemother. 1994; 38: 2311-2316.
41. Piers KL, Hancock RE. The interaction of a recombinant cecropin/melittin hybrid peptide with the outer membrane of Pseudomonas aeruginosa. Mol. Microbiol. 1994; 12: 951-958.
42. Hancock RE. Antibacterial peptides and the outer membranes of gram-negative bacilli. J. Med. Microbiol. 1997; 46: 1-3.
43. Mak P, Siwek M, Pohl J, Dubin A. Menstrual hemocidin HbB115-146 is an acidophilic antibacterial peptide potentiating the activity of human defensins, cathelicidin and lysozyme. Am. J. Reprod. Immunol. 2007; 57: 81-91. (Pubitemid 44911609)
44. Singh PK, Tack BF, McCray PB Jr, Welsh MJ. Synergistic and additive killing by antimicrobial factors found in human airway surface liquid. Am. J. Physiol. LungCell Mol. Physiol. 2000; 279: L799-L805.
45. Nagaoka I, Hirota S, Yomogida S, Ohwada A, Hirata M. Synergistic actions of antibacterial neutrophil defensins and cathelicidins. Inflamm. Res. 2000; 49: 73-79. (Pubitemid 30123847)
46. Ulvatne H, Karoliussen S, Stiberg T, Rekdal O, Svendsen JS. Short antibacterial peptides and erythromycin act synergically against Escherichia coli. J. Antimicrob. Chemother. 2001; 48: 203-208. (Pubitemid 32755280)
47. Vorland LH, Osbakk SA, Perstolen T, Ulvatne H, Rekdal O, Svendsen JS, Gutteberg TJ. Interference of the antimicrobial peptide lactoferricin B with the action of various antibiotics against Escherichia coli and Staphylococcus aureus. Scand. J. Infect. Dis. 1999; 31: 173-177. (Pubitemid 29355527)
48. Khalil H, Chen T, Riffon R, Wang R, Wang Z. Synergy between polyethylenimine and different families of antibiotics against a resistant clinical isolate of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 2008; 52: 1635-1641. (Pubitemid 351614654)