Construction and performance of heterologous polyketide-producing K-12- and B-derived Escherichia coli

Letters in Applied Microbiology

Volumn 51, Issue 2, 2010, Pages 196-204

  Wu, J ^a,b   Boghigian, B A ^b   Myint, M ^b   Zhang, H ^b   Zhang, S ^a   Pfeifer, B A ^b  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b Tufts University   (United States)

Author keywords

BL21; E. coli; heterologous; K 12; polyketide

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; GENE EXPRESSION;

CODON BIAS; E. COLI; ESCHERICHIA COLI B; GENETIC BACKGROUNDS; GENETICALLY MODIFIED; HETEROLOGOUS PRODUCTION; MRNA STABILITY; NATURAL COMPOUNDS; POLYKETIDE BIOSYNTHESIS; POLYKETIDES; PRODUCT PRODUCTION; RARE CODON; RECOMBINANT PROTEIN PRODUCTIONS;

ESCHERICHIA COLI;

6 DEOXYERYTHRONOLIDE B; POLYKETIDE; UNCLASSIFIED DRUG; 6-DEOXYERYTHRONOLIDE B; ANTIINFECTIVE AGENT; CODON; ERYTHROMYCIN; RECOMBINANT PROTEIN;

COLIFORM BACTERIUM; GENE EXPRESSION; GENETIC ANALYSIS; GENETICALLY MODIFIED ORGANISM; PARAMETERIZATION; PERFORMANCE ASSESSMENT; PROTEIN; RECOMBINATION; SECONDARY METABOLITE;

ARTICLE; CODON; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; RNA STABILITY; ANALOGS AND DERIVATIVES; BIOSYNTHESIS; ENZYMOLOGY; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETICS; HUMAN; METABOLISM; PLASMID;

ANTI-BACTERIAL AGENTS; BIOSYNTHETIC PATHWAYS; CODON; ERYTHROMYCIN; ESCHERICHIA COLI; GENETIC ENGINEERING; HUMANS; PLASMIDS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; RNA STABILITY;

EID: 77954472829     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2010.02880.x     Document Type: Article

References (33)

1. Datsenko, K.A. Wanner, B.L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97, 6640 6645.
2. Dharmadi, Y. Gonzalez, R. (2005) A better global resolution function and a novel iterative stochastic search method for optimization of high-performance liquid chromatographic separation. J Chromatogr A 1070, 89 101.
3. Donadio, S., Staver, M.J., McAlpine, J.B., Swanson, S.J. Katz, L. (1991) Modular organization of genes required for complex polyketide biosynthesis. Science 252, 675 679.
4. Gokhale, R.S., Tsuji, S.Y., Cane, D.E. Khosla, C. (1999) Dissecting and exploiting intermodular communication in polyketide synthases. Science 284, 482 485.
5. Kane, J.F. (1995) Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr Opin Biotechnol 6, 494 500.
6. Khankal, R., Luziatelli, F., Chin, J.W., Frei, C.S. Cirino, P.C. (2008) Comparison between Escherichia coli K-12 strains W3110 and MG1655 and wild-type E. coli B as platforms for xylitol production. Biotechnol Lett 30, 1645 1653.
7. McDaniel, R., Thamchaipenet, A., Gustafsson, C., Fu, H., Betlach, M. Ashley, G. (1999) Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel "unnatural" natural products. Proc Natl Acad Sci USA 96, 1846 1851.
8. Menzella, H.G., Reisinger, S.J., Welch, M., Kealey, J.T., Kennedy, J., Reid, R., Tran, C.Q. Santi, D.V. (2006) Redesign, synthesis and functional expression of the 6-deoxyerythronolide B polyketide synthase gene cluster. J Ind Microbiol Biotechnol 33, 22 28.
9. Murli, S., Kennedy, J., Dayem, L.C., Carney, J.R. Kealey, J.T. (2003) Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production. J Ind Microbiol Biotechnol 30, 500 509. (Pubitemid 37214971)
10. Pfeifer, B.A., Admiraal, S.J., Gramajo, H., Cane, D.E. Khosla, C. (2001) Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science 291, 1790 1792.
11. Pfeifer, B.A., Wang, C.C., Walsh, C.T. Khosla, C. (2003) Biosynthesis of Yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host. Appl Environ Microbiol 69, 6698 6702.
12. Phue, J.N. Shiloach, J. (2004) Transcription levels of key metabolic genes are the cause for different glucose utilization pathways in E. coli B (BL21) and E. coli K (JM109). J Biotechnol 109, 21 30.
13. Phue, J.N., Noronha, S.B., Hattacharyya, R., Wolfe, A.J. Shiloach, J. (2005) Glucose metabolism at high density growth of E. coli B and E. coli K: differences in metabolic pathways are responsible for efficient glucose utilization in E. coli B as determined by microarrays and Northern blot analyses. Biotechnol Bioeng 90, 805 820.
14. Phue, J.N., Kedem, B., Jaluria, P. Shiloach, J. (2007) Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109. Genomics 89, 300 305.
15. Recktenwald, J., Shawky, R., Puk, O., Pfennig, F., Keller, U., Wohlleben, W. Pelzer, S. (2002) Nonribosomal biosynthesis of vancomycin-type antibiotics: a heptapeptide backbone and eight peptide synthetase modules. Microbiology 148, 1105 1118.
16. SaiSree, L., Reddy, M. Gowrishankar, J. (2001) IS186 insertion at a hot spot in the lon promoter as a basis for lon protease deficiency of Escherichia coli B: identification of a consensus target sequence for IS186 transposition. J Bacteriol 183, 6943 6946.
17. Sambrook, J., Fritsch, E. Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual.
18. Selinger, D.W., Saxena, R.M., Cheung, K.J., Church, G.M. Rosenow, C. (2003) Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation. Genome Res 13, 216 223.
19. Shiloach, J., Kaufman, J., Guillard, A.S. Fass, R. (1996) Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (lambdaDE3) and Escherichia coli JM109. Biotechnol Bioeng 49, 421 428.
20. Studier, F.W. Moffatt, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113 130.
21. Tseng, H.C., Martin, C.H., Nielsen, D.R. Prather, K.L. (2009) Metabolic engineering of Escherichia coli for enhanced production of (R)- and (S)-3-hydroxybutyrate. Appl Environ Microbiol 75, 3137 3145.
22. Walsh, C.T., O'Connor, S.E. Schneider, T.L. (2003) Polyketide- nonribosomal peptide epothilone antitumor agents: the EpoA, B, C subunits. J Ind Microbiol Biotechnol 30, 448 455.
23. Wang, J., Sarov, M., Rientjes, J., Fu, J., Hollak, H., Kranz, H., Xie, W., Stewart, A.F. et al. (2006) An improved recombineering approach by adding RecA to lambda Red recombination. Mol Biotechnol 32, 43 53.
24. Wang, Y., Boghigian, B.A. Pfeifer, B.A. (2007) Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene. Appl Microbiol Biotechnol 77, 367 373.
25. Watanabe, K., Hotta, K., Praseuth, A.P., Koketsu, K., Migita, A., Boddy, C.N., Wang, C.C., Oguri, H. et al. (2006) Total biosynthesis of antitumor nonribosomal peptides in Escherichia coli. Nat Chem Biol 2, 423 428.
26. Watanabe, K., Hotta, K., Nakaya, M., Praseuth, A.P., Wang, C.C., Inada, D., Takahashi, K., Fukushi, E. et al. (2009) Escherichia coli allows efficient modular incorporation of newly isolated quinomycin biosynthetic enzyme into echinomycin biosynthetic pathway for rational design and synthesis of potent antibiotic unnatural natural product. J Am Chem Soc 131, 9347 9353.
27. Wu, X., Jornvall, H., Berndt, K.D. Oppermann, U. (2004) Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance. Biochem Biophys Res Commun 313, 89 96.
28. Xie, X., Watanabe, K., Wojcicki, W.A., Wang, C.C. Tang, Y. (2006) Biosynthesis of lovastatin analogs with a broadly specific acyltransferase. Chem Biol 13, 1161 1169.
29. Yoon, S.H., Jeong, H., Kwon, S.-K. Kim, J.F. (2009) Genomics, biological features, and biotechnological applications of Escherichia coli B: "is B for better?". In Systems Biology and Biotechnology of Escherichia coli ed. Lee, S.Y. pp. 1 17.
30. Zhang, H., Wang, Y. Pfeifer, B.A. (2008a) Bacterial hosts for natural product production. Mol Pharm 5, 212 225.
31. Zhang, W., Li, Y. Tang, Y. (2008b) Engineered biosynthesis of bacterial aromatic polyketides in Escherichia coli. Proc Natl Acad Sci USA 105, 20683 20688.
32. Zhang, H., Boghigian, B.A. Pfeifer, B.A. (2009a) Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in Escherichia coli. Biotechnol Bioeng 105, 567 573.
33. Zhang, H., Wang, Y., Boghigian, B.A. Pfeifer, B.A. (2009b) Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in Escherichia coli. Microb Biotechnol 2, 390 394.