Refolding of the recombinant protein OmpA70 from Leptospira interrogans from inclusion bodies using high hydrostatic pressure and partial characterization of its immunological properties

Journal of Biotechnology

Volumn 148, Issue 2-3, 2010, Pages 156-162

  Fraga, Tatiana R ^a,b   Chura Chambi, Rosa M ^c   Gonçales, Amane P ^b   Morais, Zenaide M ^b   Vasconcellos, Sílvio A ^b   Morganti, Ligia ^c   Martins, Elizabeth A L ^a,b  

^a INSTITUTO BUTANTAN   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES   (Brazil)

Author keywords

High hydrostatic pressure; Inclusion bodies; Leptospira; OmpA domain; Refolding; WD 40 repeats

Indexed keywords

ANTIBODY TITERS; BIOINFORMATIC ANALYSIS; CIRCULAR DICHROISM; EARLY DIAGNOSIS; ETIOLOGICAL AGENT; GUANIDINE HYDROCHLORIDE; HIGH HYDROSTATIC PRESSURE; HUMAN POPULATION; IMMUNOLOGICAL PROPERTIES; INCLUSION BODIES; L-ARGININE; OUTER MEMBRANE PROTEIN; PARTIAL CHARACTERIZATION; RECOMBINANT PROTEIN; REDUCED GLUTATHIONE; REFOLDING; SECONDARY STRUCTURES; SEROVAR; STRUCTURAL FEATURE; THERAPEUTIC INTERVENTION; VIRULENCE FACTORS;

AMINO ACIDS; BIOINFORMATICS; BIOLOGICAL MEMBRANES; DIAGNOSIS; DICHROISM; ESCHERICHIA COLI; HYDRAULICS; HYDRODYNAMICS; HYDROSTATIC PRESSURE; POSITIVE IONS;

PROTEINS;

ARGININE; GLUTATHIONE; GLUTATHIONE DISULFIDE; GUANIDINE; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN OMPA70; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL VIRULENCE; CELL INCLUSION; CIRCULAR DICHROISM; ESCHERICHIA COLI; FEMALE; HAMSTER; HYDROSTATIC PRESSURE; IMMUNITY; LEPTOSPIRA INTERROGANS; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; CHROMATOGRAPHY, LIQUID; CIRCULAR DICHROISM; CLONING, MOLECULAR; CRICETINAE; ESCHERICHIA COLI; FEMALE; HYDROSTATIC PRESSURE; INCLUSION BODIES; LEPTOSPIRA INTERROGANS; MALE; MESOCRICETUS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY;

CRICETINAE; ESCHERICHIA COLI; LEPTOSPIRA; LEPTOSPIRA INTERROGANS; LEPTOSPIRA INTERROGANS SEROVAR COPENHAGENI;

EID: 77954459632     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.04.007     Document Type: Article

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