A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal his-tagged fusion peptide

Brazilian Journal of Medical and Biological Research

Volumn 37, Issue 8, 2004, Pages 1103-1109

  Ramos, C R R ^a,b   Abreu, P A E ^a,b   Nascimento, A L T O ^a,b   Ho, P Lee ^a,b  

^a INSTITUTO BUTANTAN   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Escherichia coli; Expression vector; Immobilized metal affinity chromatography; Protein purification

Indexed keywords

DNA FRAGMENT; HISTIDINE; HYBRID PROTEIN; PLASMID DNA; PLASMID VECTOR; RECOMBINANT PROTEIN; RESTRICTION ENDONUCLEASE; RNA POLYMERASE;

5' UNTRANSLATED REGION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIOPHAGE T7; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS;

ESCHERICHIA COLI; NEGIBACTERIA; UNIDENTIFIED BACTERIOPHAGE; VECTORS;

EID: 4444320606     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2004000800001     Document Type: Article

References (16)

1. Studier FW, Rosenberg AH, Dunn JJ & Dubendorff JW (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology, 185: 60-89.
2. Oliveira MLS, Neto JC, Krieger JE, Raw I & Ho PL (2001). Site-directed mutagenesis of bovine FGF-2 cDNA allows the production of the human-form of FGF-2 in Escherichia coli. Biotechnology Letters, 23: 1151-1157.
3. Crowe J, Dobeli H, Gentz R, Hochuli E, Stuber D & Henco K (1994). 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods in Enzymology, 31: 271-387.
4. Choi JW, Ra KS & Lee YS (1999). Enhancement of bovine growth hormone gene expression by increasing the plasmid copy number. Biotechnology Letters, 21: 1-5.
5. Chen BP & Hai T (1994). Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host. Gene, 139: 73-75.
6. Sambrook J, Fritsch EF & Maniatis T (1989). Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
7. Ribas AV, Ho PL, Tanizaki MM, Raw I & Nascimento ALTO (2000). High-level expression of tetanus fragment C-thioredoxin fusion protein in Escherichia coli. Biotechnology and Applied Biochemistry, 31: 91-94.
8. Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH & Falkow S (1977). Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene, 2: 95-113.
9. Yanisch-Perron C, Vieira J & Messing J (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33: 103-119.
10. Davison J (1984). Mechanism of control of DNA replication and incompatibility in ColE1-type plasmids. Gene, 28: 1-15.
11. Tomizawa J & Itoh T (1981). Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript. Proceedings of the National Academy of Sciences, USA, 78: 6096-6100.
12. Cesareni G, Muesing MA & Polisky B (1982). Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter. Proceedings of the National Academy of Sciences, USA, 79: 6313-6317.
13. Enomoto S, Chen G & Berman J (1998). Vectors for expressing T7 epitope- and His6 affinity-tagged fusion proteins in S. cerevisiae. Biotechniques, 24: 782-788.
14. Junqueira de Azevedo ILM, Farsky SHP, Oliveira MLS & Ho PL (2001). Molecular cloning and expression of a functional snake venom vascular endothelium growth factor (VEGF) from the Bothrops insularis pit viper. A new member of the VEGF family of proteins. Journal of Biological Chemistry, 276: 39836-39842.
15. Areas APM, Oliveira MLS, Ramos CRR, Sbrogio-Almeida ME, Raw I & Ho PIL (2002). Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli. Protein Expression and Purification, 25: 481-487.
16. Ramos CRR, Figueiredo RCR, Pertinhez TA, Vilar MM, Nascimento ALTO, Tendler M, Raw I, Spisni A & Ho PL (2003). Gene structure and M20T polymorphism of the Schistosoma mansoni Sm14 fatty acid-binding protein: Molecular, functional and immunoprotection analysis. Journal of Biological Chemistry, 278: 12745-12751.