Apoptosis and reduced cell proliferation of HL-60 cell line caused by human telomerase reverse transcriptase inhibition by siRNA

Acta Haematologica

Volumn 124, Issue 2, 2010, Pages 72-78

  Miri Moghaddam, Ebrahim ^a,b   Deezagi, Abdolkhaleg ^b   Soheili, Zahra Sohaila ^b   Shariati, Parvin ^b  

^a ZAHEDAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

Author keywords

Apoptosis; Cell proliferation; HL 60; Human telomerase reverse transcriptase; Short interfering RNA; Telomerase

Indexed keywords

SMALL INTERFERING RNA; TELOMERASE REVERSE TRANSCRIPTASE; MESSENGER RNA; TELOMERASE; TERT PROTEIN, HUMAN;

APOPTOSIS; ARTICLE; CELL GROWTH; CELL PROLIFERATION; CONTROLLED STUDY; DOWN REGULATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LEUKEMIA CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMYELOCYTE; PROTEIN EXPRESSION; ANTAGONISTS AND INHIBITORS; CELL DIVISION; ENZYME ACTIVATION; GENE THERAPY; GENETICS; HL 60 CELL LINE; LEUKEMIA, PROMYELOCYTIC, ACUTE; METABOLISM; PHYSIOLOGY; PROCEDURES;

APOPTOSIS; CELL DIVISION; ENZYME ACTIVATION; GENE THERAPY; HL-60 CELLS; HUMANS; LEUKEMIA, PROMYELOCYTIC, ACUTE; RNA, MESSENGER; RNA, SMALL INTERFERING; TELOMERASE; TRANSFECTION; GENETIC THERAPY;

EID: 77954411708     PISSN: 00015792     EISSN: None     Source Type: Journal    
DOI: 10.1159/000314960     Document Type: Article

References (37)

1. Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bac-chetti S, Haber DA, Weinberg RA: hEST2, the putative human telomerase catalytic sub-unit gene, is up-regulated in tumor cells and during immortalization. Cell 1997; 90:785-795.
2. Nakamura TM, Morin GB, Chapman KB, Weinrich SL, Andrews WH, Lingner J, Har-ley CB, Cech TR: Telomerase catalytic sub-unit homologs from fission yeast and hu-ma n. Science 1997; 277: 955-959.
3. Feng J, Funk WD, Wang SS, Weinrich SL, Avilion AA, Chiu CP, Adams RR, Chang E, Allsopp RC, Yu J, Le SY, West MD, Harley CB, Andrews WH, Greider CW, Villepon-teau B: The RNA component of human telomerase. Science 1995; 269: 1236-1241.
4. Kannan S, Tahara H, Yokozaki H, Mathew B, Nalinakumari KR, Nair MK, Tahara E: Telomerase activity in premalignant and malignant lesions of human oral mucosa. Cancer Epidemiol Biomarkers Prev 1997; 6: 413-420.
5. Ohyashiki JH, Sashida G, Tauchi T, Ohyas-hiki K: Telomeres and telomerase in hemato-logic neoplasia. Oncogene 2002; 21:680-687.
6. Hiyama E, Gollahon L, Kataoka T, Kuroi K, Yokoyama T, Gazdar AF, Hiyama K, Pi-atyszek MA, Shay JW: Telomerase activity in human breast tumors. J Natl Cancer Inst 1996; 88:116-122.
7. Bonatz G, Klapper W, Barthe A, Heidorn K, Jonat W, Krupp G, Parwaresch R: Analysis of telomerase expression and proliferative activity in the different layers of cyclic endome-trium. Biochem Biophys Res Commun 1998; 253:214-221.
8. Shay JW, Bacchetti S: A survey of telomerase activity in human cancer. Eur J Cancer 1997; 33:787-791.
9. Kyo S, Inoue M: Complex regulatory mechanisms of telomerase activity in normal and cancer cells: how can we apply them for cancer therapy? Oncogene 2002; 21: 688-697.
10. Cong YS, Wright WE, Shay JW: Human telomerase and its regulation. Microbiol Mol Biol Rev 2002; 66:407-425.
11. Zhang TC, Schmitt MT, Mumford JL: Effects of arsenic on telomerase and telomeres in relation to cell proliferation and apoptosis in human keratinocytes and leukemia cells in vitro. Carcinogenesis 2003; 24:1811-1817.
12. Liu L, Berletch JB, Green JG, Pate MS, Andrews LG, Tollefsbol TO: Telomerase inhibition by retinoids precedes cytodifferentia-tion of leukemia cells and may contribute to terminal differentiation. Mol Cancer Ther 2004; 3:1003-1009.
13. Liu J-J, Wu X-Y, Peng J, Pa n X-L , Lu H-L : A n-tiproliferation effects of oridonin on HL-60 cells. Ann Hematol 2004; 83:691-695.
14. Yamaguchi T, Takahashi H, Harada Y, Yo-shikawa M, Iyono S, Inomata M, Maruyama T, Saneyoshi M: Telomere shortening in human HL60 cells by treatment with deoxy-guanosine analogs. Nucleic Acids Symp Ser 2004; 187-188.
15. Pizzimenti S, Briatore F, Laurora S, Toaldo C, Maggio M, De Grandi M, Meaglia L, Mene-gatti E, Giglioni B, Dianzani MU, Barrera G: 4-Hydroxynonenal inhibits telomerase activity and hTERT expression in human leu-kemic cell lines. Free Radic Biol Med 2006; 40: 1578-1591.
16. Zhang Y, He DM: Effect of antisense hTERT mRNA oligodeoxynucleotide on telomerase activity of leukemic cells. Cell Biol Int 2002; 26: 427-431.
17. Hammond SM, Caudy AA, Hannon GJ: Post-transcriptional gene silencing by double-stranded RNA. Nat Rev Genet 2001; 2: 110-119.
18. de Souza Nascimento P, Alves G, Fiedler W: Telomerase inhibition by an siRNA directed against hTERT leads to telomere attrition in HT29 cells. Oncol Rep 2006; 16 : 423-428.
19. Gandellini P, Folini M, Bandiera R, De Ce-sare M, Binda M, Veronese S, Daidone MG, Zunino F, Zaffaroni N: Down-regulation of human telomerase reverse transcriptase through specific activation of RNAi pathway quickly results in cancer cell growth impairment. Biochem Pharmacol 2007; 73: 1703-1714.
20. Ning S, Fuessel S, Kotzsch M, Kraemer K, Kappler M, Schmidt U, Taubert H, Wirth MP, Meye A: siR NA-mediated dow n-reg ula-tion of survivin inhibits bladder cancer cell growth. Int J Oncol 2004; 25:1065-1071.
21. Shammas MA, Liu X, Gavory G, Raney KD, Balasubramanian S, Shmookler Reis RJ: Targeting the single-strand G-rich overhang of telomeres with PNA inhibits cell growth and induces apoptosis of human immortal cells. Exp Cel l Res 2004; 295: 204-214.
22. Tebes SJ, Johnson NC, Fiorica JV, Kruk PA: Inhibition of telomerase in ovarian cancer using siRNA technology (meeting abstracts). J Clin Oncol 2005; 23: 3182 .
23. Zhang PH, Zou L, Tu ZG: RNAi-hTERT inhibition hepatocellular carcinoma cell proliferation via decreasing telomerase activity. J Surg Res 2006; 131: 143-149.
24. Zheng JN, Sun YF, Pei DS, Liu JJ, Chen JC, Li W, Sun XQ, Shi QD, Han RF, Ma TX: Inhibition of proliferation and induction of apop-tosis in human renal carcinoma cells by anti-telomerase small interfering RNAs. Acta Biochim Biophys Sin 2006; 38:500-506.
25. Miri-Moghaddam E, Deezagi A, Soheili ZS: Downregulation of telomerase activity in human promyelocytic cell line using RNA interference. Ann Hematol 2009; 88:1169-1176.
26. Reynolds A, Leake D, Boese Q, Scaringe S, Marshall WS, Khvorova A: Rational siRNA design for RNA interference. Nat Biotechnol 2004; 22:326-330.
27. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156-159.
28. -AACt method. Methods 2001; 25:402-408.
29. Ye J, Wu YL, Zhang S, Chen Z, Guo LX, Zhou RY, Xie H: Inhibitory effect of human telom-erase antisense oligodeoxyribonucleotides on the growth of gastric cancer cell lines in variant tumor pathological subtype. World J Gastroenterol 2005; 11 : 2230-2237.
30. Griffith JD, Comeau L, Rosenfield S, Stansel RM, Bianchi A, Moss H, de Lange T: Mammalian telomeres end in a large duplex loop. Cell 1999; 97:503-514.
31. Keith WN, Bilsland A, Evans TR, Glasspool RM: Telomerase-directed molecular therapeutics. Expert Rev Mol Med 2002; 4: 1-25.
32. Saretzki G , Sitte N, Merkel U, Wurm R E , von Zglinicki T: Telomere shortening triggers a p53-dependent cell cycle arrest via accumulation of G-rich single stranded DNA fragments. Oncogene 1999; 18 : 514 8-5158 .
33. Karlseder J, Broccoli D, Dai Y, Hardy S, Lange Td: p53-and ATM-dependent apopto-sis induced by telomeres lacking TRF2. Science 1999; 283:1321-1325.
34. Saretzki G, Ludwig A, von Zglinicki T, Run-nebaum IB: Ribozyme-mediated telomer-ase inhibition induces immediate cell loss but not telomere shortening in ovarian cancer cells. Cancer Gene Ther 2001; 8: 827-834.
35. Li S, Rosenberg JE, Donjacour AA, Botch-kina IL, Hom YK, Cunha GR, Blackburn EH: Rapid inhibition of cancer cell growth induced by lentiviral delivery and expression of mutant-template telomerase RNA and an-ti-telomerase short-interfering RNA. Cancer Res 20 04; 6 4: 4833-4840.
36. Perrault SD, Hornsby PJ, Betts DH: Global gene expression response to telomerase in bovine adrenocortical cells. Biochem Bio-phys Res Commun 2005; 335:925-936.
37. Lai SR, Cunningham AP, Huynh VQ, Andrews LG, Tollefsbol TO: Evidence of extra-telomeric effects of hTERT and its regulation involving a feedback loop. Exp Cell Res 2007; 313:322-330.