Non-invasive iontophoretic delivery of enzymatically active ribonuclease A (13.6kDa) across intact porcine and human skins

Journal of Controlled Release

Volumn 145, Issue 3, 2010, Pages 203-209

  Dubey, S ^a   Kalia, Y N ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

Iontophoresis; Non invasive; Protein delivery; RNAse; Transdermal

Indexed keywords

CONTROL EXPERIMENTS; ELECTROTRANSPORT; ENZYMATIC ASSAY; FUNCTIONAL PROTEINS; HUMAN SKIN; IN-VITRO; IONTOPHORETIC DELIVERY; IONTOPHORETIC TRANSPORT; LASER SCANNING CONFOCAL MICROSCOPY; MALDI-TOF; METHYLENE BLUE; NON-INVASIVE; PORCINE SKIN; PROTEIN CONCENTRATIONS; PROTEIN DELIVERY; RHODAMINE B; RIBONUCLEASE A; SDS-PAGE; SKIN SURFACES; STEADY STATE FLUXES; TOTAL FLUX; TRANSDERMAL; TRANSDERMAL IONTOPHORESIS;

CONFOCAL MICROSCOPY; ELECTROTHERAPEUTICS; EXPERIMENTS; PERMEATION; PROTEINS;

ELECTROPHORESIS;

PARACETAMOL; RHODAMINE B; RIBONUCLEASE A; RNA; PANCREATIC RIBONUCLEASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; DRUG DISTRIBUTION; DRUG LABELING; DRUG PENETRATION; DRUG STABILITY; DRUG TRANSPORT; ENZYME ACTIVITY; ENZYME ASSAY; HUMAN; HUMAN TISSUE; IN VITRO STUDY; IONTOPHORESIS; LASER SCANNING CYTOMETRY; NON INVASIVE PROCEDURE; NONHUMAN; PRIORITY JOURNAL; SKIN PENETRATION; STEADY STATE; SWINE; ANIMAL; INTRADERMAL DRUG ADMINISTRATION; METABOLISM; PROCEDURES; SKIN; SKIN ABSORPTION;

ADMINISTRATION, CUTANEOUS; ANIMALS; HUMANS; IONTOPHORESIS; RIBONUCLEASE, PANCREATIC; SKIN; SKIN ABSORPTION; SWINE;

EID: 77954310173     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jconrel.2010.04.020     Document Type: Article

References (28)

1. Kalia Y.N., Naik A., Garrison J., Guy R.H. Iontophoretic drug delivery. Adv. Drug Deliv. Rev. 2004, 56:619-658.
2. Green P.G. Iontophoretic delivery of peptide drugs. J. Control. Release 1996, 41:33-48.
3. Schuetz Y.B., Carrupt P.-A., Naik A., Guy R.H., Kalia Y.N. Structure-permeation relationships for the non-invasive transdermal delivery of cationic peptides by iontophoresis. Eur. J. Pharm. Sci. 2006, 29:53-59.
4. Guy R.H., Kalia Y.N., Delgado-Charro M.B., Merino V., López A., Marro D. Iontophoresis: electrorepulsion and electroosmosis. J. Control. Release 2000, 64:129-132.
5. Schuetz Y.B., Naik A., Guy R.H., Vuaridel E., Kalia Y.N. Transdermal iontophoretic delivery of triptorelin in vitro. J. Pharm. Sci. 2005, 94:2175-2182.
6. Cazares-Delgadillo J., Naik A., Ganem-Rondero A., Quintanar-Guerrero D., Kalia Y.N. Transdermal delivery of cytochrome C - a 12.4kDa protein - across intact skin by constant-current iontophoresis. Pharm. Res. 2007, 24:1360-1368.
7. Abla N., Naik A., Guy R.H., Kalia Y.N. Effect of charge and molecular weight on transdermal peptide delivery by iontophoresis. Pharm. Res. 2005, 22:2069-2078.
8. Heit M.C., Williams P.L., Jayes F.L., Chang S.K., Riviere J.E. Transdermal iontophoretic peptide delivery: in vitro and in vivo studies with luteinizing hormone releasing hormone. J. Pharm. Sci. 1993, 82:240-243.
9. Raiman J., Koljonen M., Huikko K., Kostiainen R., Hirvonen J. Delivery and stability of LHRH and nafarelin in human skin: the effect of constant/pulsed iontophoresis. Eur. J. Pharm. Sci. 2004, 21:371-377.
10. Santi P., Volpato N.M., Bettini R., Catellani P.L., Massimo G., Colombo P. Transdermal iontophoresis of salmon calcitonin can reproduce the hypocalcemic effect of intravenous administration. Farmaco 1997, 52:445-448.
11. Chaturvedula A., Joshi D.P., Anderson C., Morris R.L., Sembrowich W.L., Banga A.K. In vivo iontophoretic delivery and pharmacokinetics of salmon calcitonin. Int. J. Pharm. 2005, 297:190-196.
12. Kumar S., Char H., Patel S., Piemontese D., Malick A.W., Iqbal K., Neugroschel E., Behl C.R. Effect of iontophoresis on in vitro skin permeation of an analog of growth hormone releasing factor in the hairless guinea pig model. J. Pharm. Sci. 1992, 81:635-639.
13. Suzuki Y., Iga K., Yanai Sh., Matsumoto Y., Kawase M., Fukuda T., Adachi H., Higo N., Ogawa Y. Iontophoretic pulsatile transdermal delivery of human parathyroid hormone (1-34). J. Pharm. Pharmacol. 2001, 53:1227-1234.
14. Langkjaer L., Brange J., Grodsky G.M., Guy R.H. Iontophoresis of monomeric insulin analogs in vitro: effects of insulin charge and skin pretreatment. J. Control. Release 1998, 51:47-56.
15. Pillai O., Panchagnula R. Transdermal iontophoresis of insulin: V. Effect of terpenes. J. Control. Release 2003, 88:287-296.
16. Suzuki Y., Nagase Y., Iga K., Yanai S., Kawase M., Fukuda T., Oka M., Yanai S., Matsumoto Y., Nakagawa S., Fukuda T., Adachi H., Higo N., Ogawa Y. Prevention of bone loss in ovariectomized rats by pulsatile iontophoretic administration of human PTH (1-34). J. Pharm. Sci. 2002, 91:350-361.
17. Schuetz Y.B., Naik A., Guy R.H., Vuaridel E., Kalia Y.N. Transdermal iontophoretic delivery of vapreotide acetate across porcine skin in vitro. Pharm. Res. 2005, 22:1305-1312.
18. Santoro J., Gonzalez C., Bruix M., Neira J.L., Nieto J.L., Herranz J., Rico M. High-resolution three-dimensional structure of ribonuclease A in solution by nuclear magnetic resonance spectroscopy. J. Mol. Biol. 1993, 229:722-734.
19. Greiner-Stoeffele T., Grunow M., Hahn U. A general ribonuclease assay using methylene blue. Anal. Biochem. 1996, 240:24-28.
20. Laemmli U.K. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970, 277:680-685.
21. Smith M.L., Carski T.R., Griffin C.W. Modification of fluorescent-antibody procedures employing crystalline tetramethylrhodamine isothiocyanate. J. Bacteriol. 1962, 83:1358-1359.
22. Kalia Y.N., Pirot F., Guy R.H. Homogeneous transport in a heterogeneous membrane: water diffusion across human stratum corneum in vivo. Biophys. J. 1996, 71:2692-2700.
23. Pirot F., Kalia Y.N., Stinchcomb A.L., Keating G., Bunge A., Guy R.H. Characterizing the permeability barrier of human skin in vivo. Proc. Natl Acad. Sci. U.S.A. 1997, 94:1562-1567.
24. Moser K., Kriwet K., Froehlich C., Kalia Y.N., Guy R.H. Supersaturation: enhancement of skin penetration and permeation of a lipophilic drug. Pharm. Res. 2001, 18:1006-1011.
25. Taüber U., Rost K.L. Esterase activity of the skin including species variation. Skin Pharmacokinetics 1987, vol. 1:170-183. Karger, Basel. B. Shroot, H. Schaefer (Eds.).
26. Kasting G.B., Keister J.C. Applications of electrodiffusion theory for a homogeneous membrane to iontophoretic transport through skin. J. Control. Release 1989, 8:195-210.
27. Dick I.P., Scott R.C. Pig ear skin as an in vitro model for human skin permeability. J. Pharm. Pharmacol. 1992, 44:640-645.
28. Jacobi U., Kaiser M., Toll R., Mangelsdorf S., Audring H., Otberg N., Sterry W., Lademann J. Porcine ear skin: an in vitro model for human skin. Skin Res. Technol. 2007, 13:19-24.