Fasudil attenuates lipopolysaccharide-induced acute lung injury in mice through the Rho/Rho kinase pathway

Medical Science Monitor

Volumn 16, Issue 4, 2010, Pages

  Li, Yan ^a   Wu, Yiling ^b   Wang, Zhi ^c   Zhang, Xuan hong ^a   Wu, Wei kang ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b Hosp Hebei Yiling Med Res Academy   (China)

^c SUN YAT SEN UNIVERSITY   (China)

Author keywords

Acute lung injury; Fasudil; Lipopolysaccharide (LPS, endotoxin); Polymorphonuclear leukocyte (neutrophil); Rho kinase; Vascular endothelial barrier

Indexed keywords

FASUDIL; LIPOPOLYSACCHARIDE; MYELOPEROXIDASE; RHO ASSOCIATED COILED COIL FORMING PROTEIN KINASE; RHO KINASE; UNCLASSIFIED DRUG; 1 (5 ISOQUINOLINESULFONYL) 2 METHYLPIPERAZINE; DRUG DERIVATIVE; MESSENGER RNA; PROTEIN KINASE INHIBITOR; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; ROCK2 PROTEIN, MOUSE;

ACUTE LUNG INJURY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BREATHING RATE; CLINICAL EVALUATION; CONTROLLED STUDY; ENZYME ACTIVITY; HISTOPATHOLOGY; INJURY SCALE; LUNG EDEMA; LUNG HOMOGENATE; LUNG LAVAGE; LUNG WEIGHT; MOUSE; NEUTROPHIL CHEMOTAXIS; NEUTROPHIL COUNT; NONHUMAN; RNA ANALYSIS; RNA EXTRACTION; TREATMENT OUTCOME; ANIMAL; C57BL MOUSE; CELL MOTION; DRUG EFFECT; ENZYMOLOGY; GENE EXPRESSION REGULATION; LUNG; MALE; METABOLISM; NEUTROPHIL; ORGAN SIZE; PATHOLOGY; VASCULAR ENDOTHELIUM;

1-(5-ISOQUINOLINESULFONYL)-2-METHYLPIPERAZINE; ANIMALS; BRONCHOALVEOLAR LAVAGE; CELL MOVEMENT; ENDOTHELIUM, VASCULAR; GENE EXPRESSION REGULATION, ENZYMOLOGIC; LIPOPOLYSACCHARIDES; LUNG; MALE; MICE; MICE, INBRED C57BL; NEUTROPHILS; ORGAN SIZE; PROTEIN KINASE INHIBITORS; RHO GTP-BINDING PROTEINS; RHO-ASSOCIATED KINASES; RNA, MESSENGER;

EID: 77954258423     PISSN: 12341010     EISSN: 16433750     Source Type: Journal    
DOI: None     Document Type: Article

References (52)

1. Wyncoll DL, Evans TW: Acute respiratory distress syndrome. Lancet, 1999; 354: 497-501
2. Abraham E: Neutrophils and acute lung injury. Crit Care Med, 2003; 31(Suppl.4): S195-99
3. Gasparović V, Gornik I, Ivanović D: Sepsis syndrome in Croatian intensive care units: piloting a national comparative clinical database. Croat Med J, 2006; 47: 404-9
4. Angus DC, Linde-Zwirble WT, Lidicker J et al: Epidemiology of severe sepsis in the United States: analysis of incidence outcome and associated costs of care. Crit Care Med, 2001; 29: 1303-10
5. Azghani AO, Miller EJ, Peterson BT: Virulence factors from Pseudomonas aeruginosa increase lung epithelial permeability. Lung, 2000; 178: 261-69
6. Doerschuk CM, Mizgerd JP, Kubo H et al: Adhesion molecules and cellular biomechanical changes in acute lung injury. Chest, 1999; 116(Suppl.1): 37S-43S
7. Hordijk PL: Endothelial signalling events during leukocyte transmigration. FEBS J, 2006; 273: 4408-15
8. Mafra de Lima F, Naves KT, Machado AH et al: Lung inflammation and endothelial cell damage are decreased after treatment with phototherapy (PhT) in a model of acute lung injury induced by Escherichia coli lipopolysaccharide in the rat. Cell Biol Int, 2009; 33: 1212-21
9. Ware LB, Matthay MA: The acute respiratory distress syndrome. N Engl J Med, 2000; 342: 1334-49
10. Bachofen M, Weibel ER: Structural alterations of lung parenchyma in the adult respiratory distress syndrome. Clin Chest Med, 1982; 3: 35-56
11. Maniatis NA, Kotanidou A, Catravas JD, Orfanos SE: Endothelial pathomechanisms in acute lung injury. Vascul Pharmacol, 2008; 49: 119-33
12. Chignard M, Balloy V: Neutrophil recruitment and increased permeability during acute lung injury induced by lipopolysaccharide. Am J Physiol Lung Cell Mol Physiol, 2000; 279: L1083-90
13. Shimizu Y, Dobashi K, Iizuka K et al: Contribution of small GTPase Rho and its target protein rock in a murine model of lung fibrosis. Am J Respir Crit Care Med, 2001; 163: 210-17
14. Seto M, Sasaki Y, Hidaka H, Sasaki Y: Effects of HA1077, a protein kinase inhibitor, on myosin phosphorylation and tension in smooth muscle. Eur J Pharmacol, 1991; 195: 267-72
15. Nagumo H, Sasaki Y, Ono Y et al: Rho kinase inhibitor HA-1077 prevents Rho-mediated myosin phosphatase inhibition in smooth muscle cells. Am J Physiol Cell Physiol, 2000; 278: C57-65
16. Shimokawa H, Seto M, Katsumata N et al: Rho-kinase-mediated pathway induces enhanced myosin light chain phosphorylations in a swine model of coronary artery spasm. Cardiovasc Res, 1999; 43: 1029-39
17. Tasaka S, Koh H, Yamada W et al: Attenuation of endotoxin-induced acute lung injury by the Rho-associated kinase inhibitor, Y-27632. Am J Respir Cell Mol Biol, 2005; 32: 504-10
18. Mohri M, Shimokawa H, Hirakawa Y et al: Rho-kinase inhibition with intracoronary fasudil prevents myocardial ischemia in patients with coronary microvascular spasm. J Am Coll Cardiol, 2003; 41: 15-19
19. Satoh S, Utsunomiya T, Tsurui K et al: Pharmacological profile of hydroxy fasudil as a selective rho kinase inhibitor on ischemic brain damage. Life Sci, 2001; 69: 1441-53
20. Yada, Toyotaka, Shimokawa et al: Beneficial effect of hydroxyfasudil, a specific Rho-kinase inhibitor, on ischemia/reperfusion injury in canine coronary microcirculation in vivo. J Am Coll Cardiol, 2005; 45: 599-607
21. Ying H, Biroc SL, Li WW et al: The Rho kinase inhibitor fasudil inhibits tumor progression in human and rat tumor models. Mol Cancer Ther, 2006; 5: 2158-64
22. Takekazu K, Atsushi Y, Nobuyoshi I, Toshihide Y: The therapeutic effects of Rho-ROCK inhibitors on CNS disorders. Ther Clin Risk Manag, 2008; 4: 605-15
23. Starcher B, Williams I: A method for intratracheal instillation of endotoxin into the lungs of mice. Lab Anim, 1989; 23: 234-40
24. Honda K, Kobayashi H, Hataishi R et al: Inhaled nitric oxide reduces tyrosine nitration after lipopolysaccharide instillation into lungs of rats. Am J Respir Crit Care Med, 1999; 160: 678-88
25. Jansson AH, Eriksson C, Wang X: Lung inflammatory responses and hyperinflation induced by an intratracheal exposure to lipopolysaccharide in rats. Lung, 2004; 182: 163-71
26. Tang WW, Yi ES, Remick DG et al: Intratracheal injection of endotoxin and cytokines: IX. Contribution of CD11a/ICAM-1 to neutrophil emigration. Am J Physiol Lung Cell Mol Physiol, 1995; 269: L653-59
27. Hirano S: Migratory response of PMN after intraperitoneal and intratracheal administration of lipopolysaccharide. Am J Physiol Lung Cell Mol Physiol, 1996; 270: L836-45
28. Wu Q, Gui P, Yao S, Xiang H: Expression of integrin alpha(v)beta(6) in rats with ventilator-induced lung injury and the attenuating effect of synthesized peptide S247. Med Sci Monit, 2008; 14(2): BR41-48
29. Goldblum SE, Wu KM, Jay M: Lung myeloperoxidase as a measure of pulmonary leukostasis in rabbits. J Appl Physiol, 1985; 59: 1978-85
30. Hu QJ, Xiong SD, Zhang HL et al: Altered surfactant protein A gene expression and protein homeostasis in rats with emphysematous changes. Chin Med J, 2008; 12: 1177-82
31. Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 2001; 25: 402-8
32. Davies SP, Reddy H, Caivano M, Cohen P: Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J, 2000; 351: 95-105
33. Ishizaki T, Uehata M, Tamechika I et al: Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol, 2000; 57: 976-83
34. Schaafsma D, Bos IS, Zuidhof AB et al: The inhaled Rho kinase inhibitor Y-27632 protects against allergen-induced acute bronchoconstriction, airway hyperresponsiveness, and inflammation. Am J Physiol Lung Cell Mol Physiol, 2008; 295: L214-19
35. Ohashi K L, Tung D K, Wilson J et al: Transvascular and interstitial migration of neutrophils in rat mesentery. Microcirculation, 1996; 3: 199-210
36. Bazzoni G, Dejana E: Endothelial cell-to-cell junctions: Molecular organization and role in vascular homeostasis. Physiol Rev, 2004; 84: 869-901
37. Waschke J, Curry FE, Adamson RH, Drenckhahn D: Regulation of actin dynamics is critical for endothelial barrier functions. Am J Physiol Heart Circ Physiol, 2005; 288: H1296-305
38. Wojciak-Stothard B, Ridley AJ: Rho GTPases and the regulation of endothelial permeability. Vascul Pharmacol, 2003; 39: 187-99
39. Mehta D, Malik AB: Signaling mechanisms regulating endothelial permeability. Physiol Rev, 2006; 86: 279-367
40. Essler M, Amano M, Kruse HJ et al: Thrombin inactivates myosin light chain phosphatase via Rho and its target Rho kinase in human endothelial cells. J Biol Chem, 1998; 273: 21867-74
41. van Nieuw Amerongen GP, van Delft S, Vermeer MA et al: Activation of RhoA by thrombin in endothelial hyperpermeability: role of Rho kinase and protein tyrosine kinases. Circ Res, 2000; 87: 335-40
42. Essler M, Hermann K, Amano M et al: Pasteurella multocida toxin increases endothelial permeability via Rho kinase and myosin light chain phosphatase. J Immunol, 1998; 161: 5640-46
43. Danner RL, Elin RJ, Hosseini JM et al: Endotoxemia in human septic shock. Chest, 1991; 99: 169-75
44. Bannerman DD, Goldblum SE: Direct effects of endotoxin on the endothelium: barrier function and injury. Lab Invest, 1999; 79: 1181-99
45. Essler M, Staddon JM, Weber PC, Aepfelbacher M: Cyclic AMP blocks bacterial lipopolysaccharide-induced myosin light chain phosphorylation in endothelial cells through inhibition of Rho/Rho kinase signaling. J Immunol, 2000; 164: 6543-49
46. Saito H, Minamiya Y, Saito S, Ogawa J: Endothelial Rho and Rho kinase regulate neutrophil migration via endothelial myosin light chain phosphorylation. J Leukoc Biol, 2002; 72: 829-36
47. Uehata M, Ishizaki T, Satoh H et al: Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature, 1997; 389: 990-94
48. Schlegel N, Baumer Y, Drenckhahn D, Waschke J: Lipopolysaccharideinduced endothelial barrier breakdown is cyclic adenosine monophosphate dependent in vivo and in vitro. Crit Care Med, 2009; 37: 1735-43
49. Chen J, Liu X, Shu Q, Li S, Luo F: Ghrelin attenuates lipopolysaccharide-induced acute lung injury through NO pathway. Med Sci Monit, 2008; 14(7): BR141-46
50. Slotta JE, Braun OO, Menger MD, Thorlacius H: Fasudil, a Rho-kinase inhibitor, inhibits leukocyte adhesion in inflamed large blood vessels in vivo. Inflamm Res, 2006; 55: 364-67
51. Thorlacius K, Slotta JE, Laschke MW et al: Protective effect of fasudil, a Rho-kinase inhibitor, on chemokine expression, leukocyte recruitment, and hepatocellular apoptosis in septic liver injury. J Leukoc Biol, 2006; 79: 923-31
52. Mong PY, Wang Q: Activation of Rho Kinase Isoforms in Lung Endothelial Cells during Inflammation. J Immunol, 2009; 182: 2385-94