Isolation of mitochondria from the CNS

Current Protocols in Neuroscience

Volumn , Issue SUPPL. 52, 2010, Pages

  Kristian, Tibor ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

brain; mitochondria; nonsynaptic; Percoll; synaptosomes

Indexed keywords

NITROGEN;

ANIMAL TISSUE; BRAIN MITOCHONDRION; BRAIN TISSUE; CELL DISRUPTION; CELL FRACTIONATION; CELL ISOLATION; CENTRAL NERVOUS SYSTEM; DECOMPRESSION; DENSITY GRADIENT CENTRIFUGATION; MALE; METHODOLOGY; MITOCHONDRIAL RESPIRATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; REVIEW; SYNAPTOSOME;

ANIMALS; BRAIN; CELL FRACTIONATION; CENTRIFUGATION, DENSITY GRADIENT; MITOCHONDRIA; NITROGEN COMPOUNDS; RATS; SUBCELLULAR FRACTIONS; SYNAPTOSOMES; TIME FACTORS;

EID: 77954195254     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/0471142301.ns0722s52     Document Type: Review

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34. Sims, and, Anderson, 2008. See above. This paper offers detailed protocols for isolating mitochondria from brain tissues of different sample sizes by using a modification of Percoll gradient centrifugation.
35. Graham, 1999. See above. Describes the principles of gradient centrifugation and use of variety of density media that can be used to prepare pure fractions of mitochondria.
36. Brown et al., 2004. See above. This paper describes the protocol that uses the nitrogen cavitation to isolate the whole mitochondrial population (nonsynaptic and synaptic mitochondria) from brain homogenate.