MG132 as a proteasome inhibitor induces cell growth inhibition and cell death in A549 lung cancer cells via influencing reactive oxygen species and GSH level

Human and Experimental Toxicology

Volumn 29, Issue 7, 2010, Pages 607-614

  Han, Yong Hwan ^a   Park, Woo Hyun ^a  

^a Chonbuk National University   (South Korea)

Author keywords

A549 lung cancer; apoptosis; cell cycle; GSH; MG132; ROS

Indexed keywords

BENZYLOXYCARBONYLLEUCYLLEUCYLLEUCINAL; GLUTATHIONE; OXYGEN; PROTEASOME INHIBITOR; REACTIVE OXYGEN METABOLITE; ANTINEOPLASTIC AGENT; BENZYLOXYCARBONYLLEUCYL-LEUCYL-LEUCINE ALDEHYDE; ENZYME INHIBITOR; LEUPEPTIN; PROTEASOME;

APOPTOSIS; ARTICLE; CANCER CELL; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL DEATH; CELL GROWTH; CONTROLLED STUDY; FLOW CYTOMETRY; GROWTH INHIBITION; HUMAN; HUMAN CELL; IC 50; LUNG CANCER; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; ADENOCARCINOMA; CELL CYCLE; CELL PROLIFERATION; DRUG EFFECTS; ENZYMOLOGY; LUNG NEOPLASMS; METABOLISM; NONPARAMETRIC TEST; TUMOR CELL CULTURE;

ADENOCARCINOMA; ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; ENZYME INHIBITORS; HUMANS; LEUPEPTINS; LUNG NEOPLASMS; PROTEASOME ENDOPEPTIDASE COMPLEX; REACTIVE OXYGEN SPECIES; STATISTICS, NONPARAMETRIC; TUMOR CELLS, CULTURED;

EID: 77953834412     PISSN: 09603271     EISSN: 14770903     Source Type: Journal    
DOI: 10.1177/0960327109358733     Document Type: Article

References (29)

1. Gonzalez C., Sanz-Alfayate G., Agapito MT, et al. Significance of ROS in oxygen sensing in cell systems with sensitivity to physiological hypoxia. Respir Physiol Neurobiol 2002; 132: 17-41.
2. Baran CP, Zeigler MM, Tridandapani S. and Marsh CB The role of ROS and RNS in regulating life and death of blood monocytes. Current pharmaceutical design 2004; 10: 855-866.
3. Zorov DB, Juhaszova M., Sollott SJ Mitochondrial ROS-induced ROS release: An update and review. Biochim Biophys Acta 2006; 1757: 509-517.
4. Poot M., Teubert H., Rabinovitch PS and Kavanagh TJ De novo synthesis of glutathione is required for both entry into and progression through the cell cycle. J Cell Physiol 1995; 163: 555-560.
5. Schnelldorfer T., Gansauge S., Gansauge F., et al. Glutathione depletion causes cell growth inhibition and enhanced apoptosis in pancreatic cancer cells. Cancer 2000; 89: 1440-1447.
6. Lauterburg BH Analgesics and glutathione. Am J Ther 2002; 9: 225-233.
7. Orlowski RZ The role of the ubiquitin-proteasome pathway in apoptosis. Cell Death Differ 1999; 6: 303-313.
8. Voges D., Zwickl P., Baumeister W. The 26S proteasome: a molecular machine designed for controlled proteolysis. Annu Rev Biochem 1999; 68: 1015-1068.
9. Lee DH, Goldberg AL Proteasome inhibitors: valuable new tools for cell biologists. Trends Cell Biol 1998; 8: 397-403.
10. Wu HM, Chi KH, Lin WW Proteasome inhibitors stimulate activator protein-1 pathway via reactive oxygen species production. FEBS Lett 2002; 526: 101-105.
11. Perez-Galan P., Roue G., Villamor N., et al. The proteasome inhibitor bortezomib induces apoptosis in mantle-cell lymphoma through generation of ROS and Noxa activation independent of p53 status. Blood 2006; 107: 257-264.
12. Ling YH, Liebes L., Zou Y. and Perez-Soler R. Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic response to Bortezomib, a novel proteasome inhibitor, in human H460 non-small cell lung cancer cells. J Biol Chem 2003; 278: 33714-33723.
13. Qiu JH, Asai A., Chi S., et al. Proteasome inhibitors induce cytochrome c-caspase-3-like protease-mediated apoptosis in cultured cortical neurons. J Neurosci 2000; 20: 259-265.
14. Petty RD, Nicolson MC, Kerr KM, Collie-Duguid E. and Murray GI Gene expression profiling in non-small cell lung cancer: from molecular mechanisms to clinical application. Clin Cancer Res 2004; 10: 3237-3248.
15. Han YH, Kim SZ, Kim SH and Park WH Arsenic trioxide inhibits growth of As4.1 juxtaglomerular cells via cell cycle arrest and caspase-independent apoptosis. Am J Physiol Renal Physiol 2007; 293: F511-520.
16. Han YH, Kim SZ, Kim SH and Park WH Pyrogallol as a glutathione depletor induces apoptosis in HeLa cells. Int J Mol Med 2008; 21: 721-730.
17. Yang J., Liu X., Bhalla K., et al. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science 1997; 275: 1129-1132.
18. Kastan MB, Bartek J. Cell-cycle checkpoints and cancer. Nature 2004; 432: 316-323.
19. Wu WK, Wu YC, Yu L., et al. Induction of autophagy by proteasome inhibitor is associated with proliferative arrest in colon cancer cells. Biochem Biophys Res Commun 2008; 374: 258-263.
20. Yan XB, Yang DS, Gao X., et al. Caspase-8 dependent osteosarcoma cell apoptosis induced by proteasome inhibitor MG132. Cell Biol Int 2007; 31: 1136-1143.
21. Zhao P., Zhong W., Ying X., et al. Manganese chloride-induced G0/G1 and S phase arrest in A549 cells. Toxicology 2008; 250: 39-46.
22. Zhang W., Tong Q., Li S., Wang X. and Wang Q. MG-132 inhibits telomerase activity, induces apoptosis and G(1) arrest associated with upregulated p27kip1 expression and downregulated survivin expression in gastric carcinoma cells. Cancer Invest 2008; 26: 1032-1036.
23. Adams J. The proteasome: a suitable antineoplastic target. Nat Rev Cancer 2004; 4: 349-360.
24. Wu HM, Wen HC, Lin WW Proteasome inhibitors stimulate interleukin-8 expression via Ras and apoptosis signal-regulating kinase-dependent extracellular signal-related kinase and c-Jun N-terminal kinase activation. Am J Respir Cell Mol Biol 2002; 27: 234-243.
25. Emanuele S., Calvaruso G., Lauricella M., et al. Apoptosis induced in hepatoblastoma HepG2 cells by the proteasome inhibitor MG132 is associated with hydrogen peroxide production, expression of Bcl-XS and activation of caspase-3. Int J Oncol 2002; 21: 857-865.
26. Estrela JM, Ortega A., Obrador E. Glutathione in cancer biology and therapy. Crit Rev Clin Lab Sci 2006; 43: 143-181.
27. Higuchi Y. Glutathione depletion-induced chromosomal DNA fragmentation associated with apoptosis and necrosis. J Cell Mol Med 2004; 8: 455-464.
28. Park WH, Han YW, Kim SH and Kim SZ A superoxide anion generator, pyrogallol induces apoptosis in As4.1 cells through the depletion of intracellular GSH content. Mutat Res 2007; 619: 81-92.
29. Jahngen-Hodge J., Obin MS, Gong X., et al. Regulation of ubiquitin-conjugating enzymes by glutathione following oxidative stress. J Biol Chem 1997; 272: 28218-28226.