Experimental identification of microRNA targets on the 3' untranslated region of human FMR1 gene

Journal of Neuroscience Methods

Volumn 190, Issue 1, 2010, Pages 34-38

  Yi, Yong Hong ^a   Sun, Xun Sha ^a   Qin, Jia Ming ^a   Zhao, Qi Hua ^a   Liao, Wei Ping ^a   Long, Yue Sheng ^a  

^a GUANGZHOU MEDICAL UNIVERSITY   (China)

Author keywords

3' untranslated region; FMR1; Fragile X syndrome; MicroRNA; Translation regulation

Indexed keywords

FIREFLY LUCIFERASE; FRAGILE X MENTAL RETARDATION PROTEIN; MICRORNA; MICRORNA 142; MICRORNA 19A; MICRORNA 19B; MICRORNA 302B; MICRORNA 323 3P; UNCLASSIFIED DRUG; 3' UNTRANSLATED REGION; FMR1 PROTEIN, HUMAN; GREEN FLUORESCENT PROTEIN; MESSENGER RNA;

3' UNTRANSLATED REGION; ARTICLE; BASE PAIRING; CELL STRAIN HEK293; CONTROLLED STUDY; ENZYME ASSAY; FRAGILE X SYNDROME; GENE CONTROL; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE IDENTIFICATION; GENE MUTATION; GENE REPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN RNA BINDING; REPORTER GENE; TRANSLATION REGULATION; ANIMAL; CELL LINE; CHEMISTRY; COMPUTER PROGRAM; COMPUTER SIMULATION; FIREFLY; GENE SILENCING; GENETICS; MUTATION; NUCLEOTIDE SEQUENCE;

3' UNTRANSLATED REGIONS; ANIMALS; BASE SEQUENCE; CELL LINE; COMPUTER SIMULATION; FIREFLIES; FRAGILE X MENTAL RETARDATION PROTEIN; GENE EXPRESSION REGULATION; GENE SILENCING; GREEN FLUORESCENT PROTEINS; HUMANS; LUCIFERASES, FIREFLY; MICRORNAS; MUTATION; RNA, MESSENGER; SOFTWARE;

EID: 77953698398     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2010.04.022     Document Type: Article

References (30)

1. Ambros V. The functions of animal microRNAs. Nature 2004, 431:350-355.
2. Ashley C.T., Wilkinson K.D., Reines D., Warren S.T. FMR1 protein: conserved RNP family domains and selective RNA binding. Science 1993, 262:563-566.
3. Bagga S., Bracht J., Hunter S., Massirer K., Holtz J., Eachus R., et al. Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation. Cell 2005, 122:553-563.
4. Bartel D.P. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004, 116:281-297.
5. Bartel D.P., Chen C.Z. Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs. Nat Rev Genet 2004, 5:396-400.
6. Conne B., Stutz A., Vassalli J.D. The 3' untranslated region of messenger RNA: a molecular 'hotspot' for pathology?. Nat Med 2000, 6:637-641.
7. De Boulle K., Verkerk A.J., Reyniers E., Vits L., Hendrickx J., Van Roy B., et al. A point mutation in the FMR-1 gene associated with fragile X mental retardation. Nat Genet 1993, 3:31-35.
8. Dostie J., Mourelatos Z., Yang M., Sharma A., Dreyfuss G. Numerous microRNPs in neuronal cells containing novel microRNAs. RNA 2003, 9:180-186.
9. Eberhart D.E., Malter H.E., Feng Y., Warren S.T. The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals. Hum Mol Genet 1996, 5:1083-1091.
10. Gedeon A.K., Baker E., Robinson H., Partington M.W., Gross B., Manca A., et al. Fragile X syndrome without CCG amplification has an FMR1 deletion. Nat Genet 1992, 1:341-344.
11. Hayashi K., Chuva de Sousa Lopes S.M., Kaneda M., Tang F., Hajkova P., Lao K., et al. MicroRNA biogenesis is required for mouse primordial germ cell development and spermatogenesis. PLoS ONE 2008, 3:e1738.
12. He L., Hannon G.J. MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet 2004, 5:522-531.
13. Hirst M., Grewal P., Flannery A., Slatter R., Maher E., Barton D., et al. Two new cases of FMR1 deletion associated with mental impairment. Am J Hum Genet 1995, 56:67-74.
14. Kenneson A., Zhang F., Hagedorn C.H., Warren S.T., Reduced FMRP and increased FMR1 transcription is proportionally associated with CGG repeat number in intermediate-length and premutation carriers. Hum Mol Genet 2001, 10:1449-1454.
15. Kim J., Krichevsky A., Grad Y., Hayes G.D., Kosik K.S., Church G.M., et al. Identification of many microRNAs that copurify with polyribosomes in mammalian neurons. Proc Natl Acad Sci USA 2004, 101:360-365.
16. Lagos-Quintana M., Rauhut R., Yalcin A., Meyer J., Lendeckel W., Tuschl T. Identification of tissue-specific microRNAs from mouse. Curr Biol 2002, 12:735-739.
17. Lewis B.P., Shih I.H., Jones-Rhoades M.W., Bartel D.P., Burge C.B. Prediction of mammalian microRNA targets. Cell 2003, 115:787-798.
18. Lugenbeel K.A., Peier A.M., Carson N.L., Chudley A.E., Nelson D.L. Intragenic loss of function mutations demonstrate the primary role of FMR1 in fragile X syndrome. Nat Genet 1995, 10:483-485.
19. Nimchinsky E.A., Oberlander A.M., Svoboda K. Abnormal development of dendritic spines in FMR1 knock-out mice. J Neurosci 2001, 21:5139-5146.
20. Oberle I., Rousseau F., Heitz D., Kretz C., Devys D., Hanauer A., et al. Instability of a 550-base pair DNA segment and abnormal methylation in fragile X syndrome. Science 1991, 252:1097-1102.
21. Pieretti M., Zhang F.P., Fu Y.H., Warren S.T., Oostra B.A., Caskey C.T., et al. Absence of expression of the FMR-1 gene in fragile X syndrome. Cell 1991, 66:817-822.
22. Pietrobono R., Pomponi M.G., Tabolacci E., Oostra B., Chiurazzi P., Neri G. Quantitative analysis of DNA demethylation and transcriptional reactivation of the FMR1 gene in fragile X cells treated with 5-azadeoxycytidine. Nucleic Acids Res 2002, 30:3278-3285.
23. Pillai R.S., Bhattacharyya S.N., Artus C.G., Zoller T., Cougot N., Basyuk E., et al. Inhibition of translational initiation by Let-7 MicroRNA in human cells. Science 2005, 309:1573-1576.
24. Reiss A.L., Freund L.S., Baumgardner T.L., Abrams M.T., Denckla M.B. Contribution of the FMR1 gene mutation to human intellectual dysfunction. Nat Genet 1995, 11:331-334.
25. Siomi H., Ishizuka A., Siomi M.C. RNA interference: a new mechanism by which FMRP acts in the normal brain? What can Drosophila teach us?. Ment Retard Dev Disabil Res Rev 2004, 10:68-74.
26. Suh M.R., Lee Y., Kim J.Y., Kim S.K., Moon S.H., Lee J.Y., et al. Human embryonic stem cells express a unique set of microRNAs. Dev Biol 2004, 270:488-498.
27. Tarleton J., Richie R., Schwartz C., Rao K., Aylsworth A.S., Lachiewicz A. An extensive de novo deletion removing FMR1 in a patient with mental retardation and the fragile X syndrome phenotype. Hum Mol Genet 1993, 2:1973-1974.
28. Verkerk A.J., Pieretti M., Sutcliffe J.S., Fu Y.H., Kuhl D.P., Pizzuti A., et al. Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome. Cell 1991, 65:905-914.
29. Weiler I.J., Irwin S.A., Klintsova A.Y., Spencer C.M., Brazelton A.D., Miyashiro K., et al. X mental retardation protein is translated near synapses in response to neurotransmitter activation. Proc Natl Acad Sci USA 1997, 94:5395-5400.
30. Zovoilis A., Nolte J., Drusenheimer N., Zechner U., Hada H., Guan K., et al. Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles. Mol Hum Reprod 2008, 14:521-529.