Effects of Water Activity (aw) on Microbial Stability: As a Hurdle in Food Preservation

Water Activity in Foods: Fundamentals and Applications

Volumn , Issue , 2008, Pages 239-271

  Tapia, María S ^a   Alzamora, Stella M ^b   Chirife, Jorge ^c  

^a FACULTAD DE CIENCIAS   (Venezuela)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^c PONTIFICIA UNIVERSIDAD CATÓLICA ARGENTINA   (Argentina)

Author keywords

Effects of water activity; Food preservation; Microbial stability; Microorganisms; Toxin production

Indexed keywords

EID: 77953544486     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470376454.ch10     Document Type: Chapter

References (121)

1. Aguilera, J.M., Chirife, J., Tapia, M.S., and Welti-Chanes, J. 1990. Inventario de Alimentos de Humedad Intermedia Tradicionales de Iberoamérica. Ciudad de México: Instituto Politécnico Nacional.
2. Alzamora, S.M., Cerrutti, P., Guerrero, S., and López-Malo, A. 1995. Minimally processed fruits by combined methods. In: Food Preservation by Moisture Control-Fundamentals and Applications, eds. J. Welti-Chanes and G. Barbosa-Cánovas, pp. 463-492. Lancaster: Technomic Publishing Company.
3. Alzamora, S.M., Fito, P., López-Malo, A., Tapia, M.S., and Parada-Arias, E. 2000. Minimally processed fruit using vacuum impregnation, natural antimicrobial addition and/or high hydrostatic pressure techniques. In: Minimally Processed Fruits and Vegetables. Fundamental Aspects and Applications, eds. S.M. Alzamora, M.S. Tapia, and A. López Malo, pp. 293-315. Gaithersburg: Aspen Publishers.
4. Alzamora, S.M., Gerschenson, L.N., Cerrutti, P., and Rojas, A.M. 1989. Shelf stable pineapple for long-term non-refrigerated storage. Lebensittel-Wissenschaft und-Technologie 22(5):233-236.
5. Alzamora, S.M., Tapia, M.S., Argaíz, A., and Welti-Chanes, J. 1993. Application of combined methods technology in minimally processed fruits. Food Research International 26:125-130.
6. Alzamora, S.M., Tapia, M.S., López-Malo, A., and Welti-Chanes, J. 2003. The control of water activity. In: Food Preservation Techniques, eds. L. Bogh-Sorensen, P. Zeuthen, and H. Lelieveld, pp. 126-153. Cambridge, England: CRC Press.
7. Baird-Parker, A.C., and Freame, B. 1967. Combined effects of water activity, pH and temperature on the growth of Clostridium botulinum from spore and vegetative cell inocula. Journal of Applied Bacteriology 30(3):420-429.
8. Ballesteros, S.A., Chirife, J., and Bozzini, J.P. 1993. Specific solute effects on Staphylococcus aureus cells subjected to reduced water activity. International Journal of Food Microbiology 20(2):51-66.
9. Baranyi, J., and Roberts, T.A. 1994. Predictive microbiology: Quantitative microbial ecology. Food Microbiology 10:43-59.
10. Beuchat, L.R. 1981. Microbial stability as affected by water activity. Cereal Foods World 26(7):345-349.
11. Beuchat, L.R. 1983. Influence of water activity on growth, metabolic activities and survival of yeasts and molds. Journal of Food Protection 46:135-141.
12. Beuchat, L.R. 1987. Influence of water activity on sporulation, germination, outgrowth and toxin production. In: Water Activity: Theory and Applications to Food, eds. L.B. Rockland and L.R. Beuchat, pp. 137-151. New York: Marcel Dekker.
13. Beuchat, L.R. 2002. Water activity and microbial stability. Fundamentals of Water Activity, IFT Continuing Education Committee, June 14-15, Anaheim, CA.
14. Blom, H., Nerbrink, E., Dainty, R., Hagtvedt, T., Borch, E., Nissen, H., and Nesbakken, T. 1997. Addition of 2.5% lactate and 0.25% acetate controls growth of Listeria monocytogenes in vacuum-packed, sensory acceptable servelat sausage and cooked ham stored at 4°C. International Journal of Food Microbiology 38:71-76
15. Briozzo, J., Amato de Lagarde, E., Chirife, J., and Parada, J.L. 1983. Effect of water activity and pH on growth and toxin production by C. botulinum type G. Applied Environmental Microbiology 51(4):844-848.
16. Brown, A.D. 1974. Microbial water relations: Features of the intracellular composition of sugar-tolerant yeasts. Journal of Bacteriology 118(3):769-777.
17. Brown, A.D., and Melling, J. 1971. Inhibition and destruction of microorganisms by heat. In: Inhibition and Destruction of Microbial Cells, ed. W.B. Hugo, pp. 1-37. London: Academic Press.
18. Buchanan, R.L. 1993. Developing and distributing user(friendly application software. Journal of Industrial Microbiology 12(3-5):251-255.
19. Buchanan, R.L., and Bagi, L.K. 1997. Effect of water activity and humectant identity on the growth kinetics of Escherichia coli O157:H7. Food Microbiology 14(5):413-423.
20. Buera, M.P., Jouppila, K., Roos, Y.H., and Chirife, J. 1998. Differential scanning calorimetry glass transition temperatures of white bread and mold growth in the putative glassy state. Cereal Chemistry 75(1):64-69.
21. Cerrutti, P., Alzamora, S.M., and Vidales, S.L. 1997. Vanillin as antimicrobial for producing shelf-stable strawberry purée. Journal of Food Science 62:608-610.
22. Chen, N., and Shelef, L.A. 1992. Relationship between water activity, salts of lactic acid, and growth of Listeria monocytogenes in a meat model system. Journal of Food Protection 55(8):574-578.
23. Chirife, J. 1993. Physicochemical aspects of food preservation by combined factors. Food Control 4:210-215.
24. Chirife, J. 1994. Specific solute effects with special reference to Staphylococcus aureus. Journal of Food Engineering 22:409-419.
25. Chirife, J., and Buera, M.P. 1994. Water activity, glass transition and microbial stability in concentrated and semi-moist food systems. Journal of Food Science 59:921-927.
26. Chirife, J., and Buera, M.P. 1996. Water activity, water glass dynamics and the control of microbiological growth in foods. Critical Reviews in Food Science and Nutrition 36:465-513.
27. Chirife, J., Buera, M.P., and González, H.H. 1999. The mobility and mold growth in glassy/rubbery substances. In: Water Management in the Design and Distribution of Quality Foods. ISOPOW 7, eds. Y.H. Roos, R.B. Leslie, and P.J. Lillford, pp. 285-298. Basel, Switzerland: Technomic Publishing.
28. Chirife J., Favetto, G., and Ferro-Fontán, C. 1984. Microbial growth at reduced water activities: Some physico-chemical properties of compatible solutes. Journal of Applied Bacteriology 56:259-264.
29. Chirife, J., Ferro-Fontan, C., and Scorza, O.C. 1981 The Intracellular water activity of bacteria in relation to the water activity of the growth medium. Journal of Applied Bacteriology 50(3):475-479.
30. Christian, J.H.B. 1963. Water activity and the growth of microorganisms. Recent Advances in Food Research 3:248-55.
31. Christian, J.H.B. 1981. Specific solute effects on microbial water relations. In: Water Activity: Influences on Food Quality, eds. L.B. Rockland and G.F. Stewart, p. 825. New York: Academic Press.
32. Corry, J.E.L. 1974. The effect of sugars and polyols on the heat resistance of Salmonellae. Journal of Applied Bacteriology 37:31-43.
33. Corry, J.E.L. 1975. The effect of water activity on the heat resistance of bacteria. In: Water Relations of Foods, ed. Ro, B. Duckworth. London: Academic Press.
34. Dodds, K.L. 1989. Combined effect of water activity and pH on inhibition of toxin production by Clostridium botulinum in cooked, vacuum-packaged potatoes. Applied Environmental Microbiology 55:656-60.
35. Earnshaw, R.G., Appleyard, J., and Hurst, R.M. 1995. Understanding physical inactivation processes: Combined preservation opportunities using heat, ultrasound and pressure. International Journal of Food Microbiology 28(2):197-219.
36. Favetto, G.J., Resnik, S.L., Chirife, J., and Ferro-Fontán, C. 1983. Statistical evaluation of water activity measurements obtained with the Vaisala Humicap humidity meter. Journal of Food Science48:534-538.
37. Fennema, O. 1996. Water and ice. In: Food Chemistry, ed. O. Fennema, pp. 17-94. New York: Marcel Dekker.
38. 2, pH, temperature and NaCl on the growth of Listeria monocytogenes. International Journal of Food Microbiology 37:37-45.
39. Fontana, A.J. 2000. Water activity's role in food safety and quality. Second NSF International Conference on Food Safety, October 11-13, 2000, Savannah, GA.
40. Fontana, A.J. 2002. Measurement of water activity. Fundamentals of Water Activity, IFT Continuing Education Committee, June 14-15, Anaheim, CA.
41. Franks, F. 1991. Water activity: A credible measure of food safety and quality? Trends in Food Science and Technology 68-72.
42. Giannuzzi, L., and Parada, J.L. 1991. Sobre el crecimiento de Staphylococcus aureus en medios de actividad acu-osa inferior a 0.86. Revista Argentina de Microbiología 23:79-85.
43. Glass, K., and Doyle, M.P. 1991. Relationship between water activity of fresh pasta and toxin production by pro-teolitic Clostridium botulinum. Journal of Food Protection 54:162-165.
44. Gould, G.W. 1985. Osmoregulation: Is the cell just a simple osmometer? The microbiological experience. In: A Discussion Conference: Water Activity: A Credible Measure of Technological Performance and Physiological Viability? Faraday Division, Royal Society of Chemistry, Girton College, Cambridge, England, July 1-3.
45. Gould, G.W. 1988. Interference with homeostasis-Foods. In: Homeostatic Mechanisms in Microorganisms, eds. J.G. Banks, R.T.G. Board, G.W. Gould, and R.W. Mittenbury. Bath, England: Bath University Press.
46. Gould, G.W. 1989. Drying, raised osmotic pressure and low water activity. In: Mechanisms of Action of Food Preservation Procedures, ed. G.W. Gould, pp. 97-118. New York: Elsevier Applied Science.
47. Gould, G.W. 1996. Methods for preservation and extension of shelf-life. International Journal of Food Microbiology 33:51-64.
48. Gould, G.W. 2000. Induced tolerance of microorganisms to stress factors. In: Minimally Processed Fruits and Vegetables. Fundamental Aspects and Applications, eds. S.M. Alzamora, M.S. Tapia, and A. López-Malo, pp. 29-37. Gaithersburg: Aspen Publishers.
49. Gould, G.W., and Measures, J.C. 1977. Water relations in single cells. Philosophical Transactions of the Royal Society of London 278:151.
50. Graham, A.F., Mason, D.R., and Peck, M.W. 1996. Predictive model of the effect of temperature, pH and sodium chloride on growth from spores of non(proteolytic Clostridium botulinum. International Journal of Food Microbiology 31:69-85.
51. Guerrero, S., Alzamora, S.M., and Gerschenson, L.N. 1994. Development of a shelf-stable banana purée by combined factors: Microbial stability. Journal Food Protection 57:902-907.
52. Gutiérrez, C., Abee, T., and Booth, I.R. 1995. Physiology of the osmotic stress response in microorganisms. International Journal of Food Microbiology 28:233-244.
53. Hocking, A.D. 1988. Strategies for microbial growth at reduced water activities. Microbiology Science 5:280-284.
54. Hocking, A.D., and Pitt, J.I. 1979. Water relations of some Penicillum species at 25°C. Transactions of the British Mycological Society 73:141-145.
55. Houtsma, P.C., Dewit, J.C., and Rombouts, F.M. 1996b. Minimum inhibitory concentration (MIC) of sodium lac-tate and sodium chloride for spoilage organisms and pathogens at different pH values and temperatures. Journal of Food Protection 59:1300-1304.
56. Houtsma, P.C., Kant-Muermans, M.L., Rombouts, F.M., and Zwietering, M.H. 1996a. Model for the combined effects of temperature, pH, and sodium lactate on growth rates of Listeria monocytogenes in broth and bologna-type sausages. Applied Environmental Microbiology 62:1616-1622.
57. Jakobsen, M., Filtenborg, O., and Bramsnaes, F. 1972, Germination and outgrowth of the bacterial spore in the presence of different solutes. Lebensittel-Wissenschaft und-Technologie 5:159-172.
58. Jayaraman, K.S. 1995. Critical review on intermediate moisture fruits and vegetables. In: Food Preservation by Moisture Control-Fundamentals and Application, eds. J. Welti-Chanes and G. Barbosa-Cénovas, pp. 411-442. Basel, Switzerland: Technomic Publishing.
59. Karel, M. 1973. Recent research and development in the field of low moisture and intermediate moisture foods. Critical Reviews in Food Technology 3:329-373.
60. Karel, M. 1976. Technology and application of new intermediate moisture foods. In: Intermediate Moisture Foods, eds. R. Davies, G.G. Birch, and K.J. Parker, pp. 4-31. London: Applied Science Publishers.
61. Karel, M. 1999. Food research tasks at the beginning of the new millennium-A personal vision. In: Water Management in the Design and Distribution of Quality Foods. ISOPOW 7, eds. Y.H. Roos, R.B. Leslie, and P.J. Lillford, pp. 535-549. Basel, Switzerland: Technomic Publishing.
62. King, D., Jr., Pitt, J.I., Beuchat, L.R., and Corry, J.E.L. 1984. Comparison of enumeration methods for molds growing in flour stored in equilibrium with relative humidities between 66 and 68%. In: Methods for the Mycological Examination of Food, pp. 143-145. New York: Plenum Press.
63. Kirby, R.M., and Davies, R. 1990. Survival of dehydrated cells of Salmonella typhimurium LT2 at high temperatures. Journal of Applied Bacteriology 68(3):241-246.
64. Kou, Y., Molitor, P.F., and Schmidt, S.J. 1999. Mobility and stability characterization of model food systems using NMR, DSC and conidia germination techniques. Journal of Food Science 64:950-959.
65. Laroche, C., and Gervais, P. 2003. Unexpected thermal destruction of dried, glass bead-immobilized microorganisms as a function of water activity. Applied and Environmental Microbiology 69(5):3015-3019.
66. Lavoie, J., and Chinachoti, P. 1995. The role of water mobility in promoting Staphylococcus aureus and Aspergillus niger activities. In: Magnetic Resonance in Food Science, eds. P.S. Belton, I. Delgadillo, A.M. Gil, and G.A. Webb, pp. 33-42. Cambridge, UK: The Royal Society of Chemistry.
67. 17 ONMR water mobility and water activity. Journal of Food Science 62:861-866.
68. Lee, R.Y., Silverman, G.J., and Munsey, D.T. 1981. Growth and enterotoxin A production by Staphylococcus aureus in precooked bacon in the intermediate moisture range. Journal of Food Science 46:1687.
69. Leistner, L. 1978. Hurdle effect and energy saving. In: Food Quality and Nutrition ed. W.K. Downey, pp. 553-557. London: Applied Science Publishers.
70. Leistner, L. 1987. Shelf-stable products and intermediate foods based on meat. In: Water Activity: Theory and Applications to Food, eds. L.B. Rockland and L.R. Beuchat, pp. 295-327. New York: Marcel Dekker.
71. Leistner, L. 1999. Combined methods for food preservation. In: Food Preservation Handbook, ed. M.S. Rahman, pp. 457-485. New York: Marcel Dekker.
72. Leistner, L. 2000. Hurdle technology in the design of minimally processed foods. In: Minimally Processed Fruits and Vegetables. Fundamental Aspects and Applications, eds. S.M. Alzamora, M.S. Tapia, and A. López-Malo, pp. 13-27. Gaithersburg: Aspen Publishers.
73. Leistner, L., and Gould, G.W. 2002. Hurdle Technologies. Combination Treatments for Food Stability, Safety and Quality. New York: Kluwer Academic/Plenum Publishers.
74. Leistner, L., and Rodel, W. 1975. The significance of water activity for microorganisms in meats. In: Water relations of Foods, ed. R.B. Duckworth, pp. 309-323. New York: Academic Press.
75. Le Meste, M., Champion, D., Roudaut, G., Blon, G., and Simatos, D. 2002. Glass transition and food technology: A critical appraisal. Journal of Food Science 67:2444-2458.
76. Lenovich, L.M. 1987. Survival and death of microorganisms as influenced by water activity. In: Water Activity: Theory and Applications to Food, eds. L.B. Rockland and L.R. Beuchat, pp. 119-136. New York: Marcel Dekker.
77. Levine, H., and Slade, L. 1992. Glass transitions in foods. In: Physical Chemistry of Foods, eds. H.G. Schwartzberg and R.W. Hartel, pp. 83-220. New York: Marcel Dekker.
78. Lotter, L.P., and Leistner, L. 1978. Minimal water activity for enterotoxin A production and growth of Staphylococcus aureus. Applied Environmental Microbiology 36:377.
79. Manas, P., Pagan, R., Leguérinel, I., Condón, S., Mafart, P. and Sala, F. 2001. Effect of sodium chloride concentration on the heat resistance and recovery of Salmonella typhimurium. International Journal of Food Microbiology 63(3):209-216.
80. Marshall, B.J., Ohye, D.F., and Christian, J.H.B. 1971. Tolerance of bacteria to high concentrations of NaCl and glycerol in the growth medium. Applied Microbiology 21:363-364.
81. McClure, P.J., Blackburn, C. de W., Cole, M.B., Curtis, P.S., Jones, J.E., Legan, J.D., Ogden, L.D., Peck, M.W., Roberts, T.A., Sutherland, J.P., and Walker, S.J. 1994. Modelling the growth, survival and death of microorganisms in foods: The UK Micromodel approach. International Journal of Food Microbiology 23:265-275.
82. McMeekin, T.A., Olley, J.N., Ross, T., and Ratkowsky, D.A. 1993. Predictive Microbiology: Theory and Application. Taunton: Research Studies Press.
83. McMeekin, T.A., Presser, K., Ratkowsky, D., Ross, T., Salter, M., and Tienungoon, S. 2000. Quantifying the hurdle concept by modelling the bacterial growth/no growth interface. International Journal of Food Microbiology 55:93-98.
84. McMeekin, T.A., and Ross, T. 2002. Predictive microbiology: Providing a knowledge(based framework for change management. International Journal of Food Microbiology 78:133-153.
85. Miles, D.W., Ross, T., Olley, J., and McMeekin, T.A. 1997. Development and evaluation of a predictive model for the effect of temperature and water activity on the growth rate of Vibrio parahaemolyticus. International Journal of Food Microbiology 38:133-142.
86. Miller, R.K., and Acuff, G.R. 1994. Sodium lactate affects pathogens in cooked beef. Journal of Food Science 59:15-19.
87. Mossel, D.A.A., Corry, J.E.L., Struijk, C.B., and Baird, R.M. 1991. Essentials of the Microbiology of Foods. A Text for Advanced Studies, pp. 91-93. Chichester: John Wiley and Sons.
88. Northolt, M.D., Frisvad, J., and Samson, R.A. 1995. Occurrence of food-borne fungi and factors for growth. In: Introduction to Foodborne Fungi, eds. R.A. Samson, E.S. Hoekstra, J.C. Frisvad, and O. Filtenborg, pp. 243-250. Wageningen: Ponsen & Looyen.
89. Notermans, S., and Heuvelman, C.J. 1983. Combined effect of water activity, pH and sub-optimal temperature on growth and enterotoxin production of Staphylococcus aureus. Journal of Food Science 48:1832.
90. O'Byrne, C., and Booth, I.R. 2002. Osmoregulation and its importance to food(borne microorganisms. International Journal of Food Microbiology 74:203-216.
91. Ohye, D.F., and Christian, J.H.B. 1966. Combined effects of temperature, pH and water activity on growth and toxin production by Clostridium botulinum types A, B and E, in Proceedings of the Fifth International Symposium on Food Microbiology, Moscow, p. 217.
92. Pichereau, V, Hartke, A., and Auffray, Y. 2000. Starvation and osmotic stress induced multiresistances influence of extracellular compounds. International Journal of Food Microbiology 55:19-25.
93. Pitt, J., and Hocking, A. 1977. Influence of solute and hydrogen ion concentration on the water relations of some xerophilic fungi. Journal of General Microbiology 101:35-40.
94. Prior, B.A., Kenyon, C.P., van der Veen, M., and Mildenhall, J.P. 1987. Water relations of Pseudomonas fluorescens. Journal of Applied Bacteriology 62:119-128.
95. Rahman, S. 1995. Food Properties Handbook, pp. 1-14. Boca Raton, FL: CRC Press.
96. Ratkowsky, D.A., and Ross, T. 1995. Modelling the bacterial growth/no growth interface. 1995. Letters in Applied Microbiology 20:29-33.
97. Roos, Y.H., and Karel, M. 1991. Plasticizing effect of water on thermal behavior and crystallization of amorphous food models. Journal of Food Science 56:38-43.
98. Rosso, L., and Robinson, T.P. 2001. A cardinal model to describe the effect of water activity on the growth of moulds. International Journal of Food Microbiology 63:265-273.
99. Russell, N.J., Evans, R.I., ter Steeg, P.F., Hellemons, J. Verheul, A., and Abee, T. 1995. Membranes as a target for stress adaptation. International Journal of Food Microbiology 28(2):255-261.
100. Scott, W.J. 1953. Water relations of Staphylococcus aureus at 30°C. Australian Journal of Biological Science 6:549-556.
101. Siderius, M.H., and Mager, W.H. 1997. General stress response: In search of a common denominator. In: Yeast Stress Responses, eds. S. Hohmann and W.H. Mager, pp. 213-230. Berlin: Springer.
102. Silverman, G.J., Munsey, D.T., Lee, C., and Ebert, E. 1983. Interrelationship between water activity, temperature and 5.5% oxygen on growth and enterotoxin secretion by Staphylococcus aureus in precooked bacon. Journal of Food Science 48:1783-1786.
103. Sinigaglia, I., Corbo, M.R., Altieri, C., and Massa, S. 2002. Response surface model for effects of temperature, water activity and pH on germination of Bacillus cereus spores. Journal of Food Safety 22:121-134.
104. Slade, L., and Levine, H. 1991. Beyond water activity: Recent advances based on an alternative approach to the assessment of food quality and safety. Critical Reviews in Food Science and Nutrition 30:115-360.
105. Slade, L., and Levine, H. 1987. Structural stability of intermediate moisture foods-A new understanding. In: Food Structure-Its Creation and Evaluation, eds. J.R. Mitchell and J.M.V. Blanshard, pp. 115-147. London: Butterworths.
106. Stewart, C.M., Cole, M.B., Legan, J.D., Slade, L., Vandeven, M.H., and Schaffner, D.W. 2002. Staphylococcus aureus growth boundaries: Moving towards mechanistic predictive models based on solute-specific effects. Applied and Environmental Microbiology 68(4):1864-1871.
107. Sumner, S.S., Sandros, T.M., Harmon, M.C., Scott, VN., and Bernard, D.T. 1991. The heat resistance of Salmonella typhimurium and L. monocytogenes in sucrose solutions of various water activities. Journal of Food Science 56:1741-1743.
108. Tapia, M., Villegas, Y., and Martínez, A. 1991. Minimal water activity for growth of Listeria monocytogenes as affected by solute and temperature. International Journal of Food Microbiology 14:333-337.
109. Tapia, M.S., Aguilera, J.M., Chirife, J., Parada, E., and Welti-Chanes, J. 1994. Identification of microbial stability factors in traditional foods from Iberoamerica. Revista Espanola de Ciencia y Tecnología de Alimentos 34:145-163.
110. Troller, J.A. 1971. Effect of water activity on enterotoxin B production and growth of Staphylococcus aureus. Applied Microbiology 21:435.
111. Troller, J.A. 1972. Effect of water activity on enterotoxin A production and growth of Staphylococcus aureus. Applied Microbiology 24:440.
112. Troller, J.A. 1987. Adaptation and growth of microorganism in environments with reduced water activity. In: Water Activity: Theory and Applications to Food, eds. L.B. Rockland and L.R. Beuchat, pp. 111-117. New York: Marcel Dekker.
113. Troller, J.A., and Stinson, J.V. 1978. Influence of water activity on the production of extracellular enzymes by Staphylococcus aureus. Applied Environmental Microbiology 35(3):521-526.
114. Vaamonde, G., Chirife, J., and Scorza, O.C. 1982. An examination of the minimal water activity for Staphy-lococcus aureus ATCC 6538 P growth in laboratory media adjusted with less conventional solutes. Journal of Food Science 47:1259-1262.
115. Valik, L., and Gorner, F. 1993. Growth of Staphylococcus aureus in pasta in relation to its water activity. International Journal of Food Microbiology 20:45-48.
116. Vilhelmsson, O., and Miller, K.J. 2002. Humectant permeability influences growth and compatible solute uptake by Staphylococcus aureus subjected to osmotic stress. Journal of Food Protection 65:1008-1015.
117. Vittadini, E., and Chinachoti, P. 2003. Effect of physico-chemical and molecular mobility parameters on Staphylococcus aureus growth. International Journal of Food Science and Technology 38:841-849.
118. Weaver, R.A., and Shelef, L.A. 1993. Antilisterial activity of sodium, potassium or calcium lactate in pork liver sausage. Journal of Food Safety 13:133-146.
119. Welti-Chanes, J., Bermúdez, D., Mújica-Paz, H., Valdez-Fragoso, A., and Alzamora, S.M. 2004. Principles of freeze concentration and freeze-drying. In: Handbook of Frozen Foods, eds. Y.H. Hui, P. Cornillon, I. Guerrero Legarreta, M. Lim, K.D. Murrell, and W.-K. Nip. New York: Marcel Dekker.
120. Welti-Chanes, J., Tapia, M.S., Aguilera, J.M., Chirife, J., Parada, E., López-Malo, A., López, L.C., and Corte, P. 1994. Classification of intermediate moisture foods consumed in Ibero-America. Revista Espanola de Ciencia y Tecnología de Alimentos 34:53-63.
121. Whiting, R.C. 1995. Microbial modeling in foods. Critical Reviews in Food Science and Nutrition 35(6):467-494.