Influence of glycosylation on the adsorption of Thermomyces lanuginosus lipase to hydrophobic and hydrophilic surfaces

European Journal of Pharmaceutical Sciences

Volumn 40, Issue 4, 2010, Pages 273-281

  Pinholt, Charlotte ^a,b   Fanø, Mathias ^a   Wiberg, Charlotte ^b   Hostrup, Susanne ^b   Bukrinsky, Jens Thostrup ^b   Frokjaer, Sven ^a   Norde, Willem ^c,d   Jorgensen, Lene ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b NOVO NORDISK A S   (Denmark)

^c WAGENINGEN UNIVERSITY   (Netherlands)

^d UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

CD; Glycosylation; Protein adsorption; SPR; Thermomyces lanuginosus lipase; TIRF

Indexed keywords

AMINO ACID; POLITEF; THERMOMYCES LANUGINOSUS LIPASE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; FUNGAL PROTEIN; IMMOBILIZED ENZYME; MUTANT PROTEIN; ORGANOSILICON DERIVATIVE; RECOMBINANT PROTEIN; SILANE DERIVATIVE; SILICON DIOXIDE;

ADSORPTION; AMINO ACID SUBSTITUTION; ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; DENATURATION; FLUORESCENCE; GLYCOSYLATION; HYDROPHILICITY; HYDROPHOBICITY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; SURFACE PLASMON RESONANCE; WILD TYPE; ASCOMYCETES; CHEMICAL PHENOMENA; CHEMISTRY; COMPARATIVE STUDY; ENZYME STABILITY; ENZYMOLOGY; KINETICS; MASS SPECTROMETRY; MOLECULAR WEIGHT; PROTEIN SECONDARY STRUCTURE; SURFACE PROPERTY;

ADSORPTION; AMINO ACID SUBSTITUTION; ASCOMYCOTA; CIRCULAR DICHROISM; ENZYME STABILITY; ENZYMES, IMMOBILIZED; FUNGAL PROTEINS; GLYCOSYLATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; LIPASE; MOLECULAR WEIGHT; MUTANT PROTEINS; ORGANOSILICON COMPOUNDS; PROTEIN STRUCTURE, SECONDARY; QUARTZ; RECOMBINANT PROTEINS; SILANES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SURFACE PLASMON RESONANCE; SURFACE PROPERTIES;

EID: 77953495699     PISSN: 09280987     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejps.2010.03.021     Document Type: Article

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