pH dependent effect of glycosylation on protein stability

European Journal of Pharmaceutical Sciences

Volumn 33, Issue 2, 2008, Pages 120-127

  Wang, Wei ^b   Antonsen, Kris ^c   Wang, Y John ^d   Wang, D Q ^a  

^a Bayer HealthCare   (United States)

^b PFIZER INC   (United States)

^c BIOMARIN PHARMACEUTICAL INC   (United States)

^d GENENTECH INC   (United States)

Author keywords

Aggregation precipitation; Protein formulation; Thermal storage stability; Unfolding

Indexed keywords

INTERLEUKIN 2 MUTEIN; RECOMBINANT INTERLEUKIN 2; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; DIFFERENTIAL SCANNING CALORIMETRY; DISULFIDE BOND; DRUG DEGRADATION; DRUG SOLUBILITY; DRUG STABILITY; DRUG STORAGE; GLYCOSYLATION; PH; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN STABILITY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TEMPERATURE; THERMOSTABILITY; ULTRAVIOLET RADIATION;

AMINO ACID SUBSTITUTION; CALORIMETRY, DIFFERENTIAL SCANNING; CYSTEINE; DRUG STORAGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HUMANS; HYDROGEN-ION CONCENTRATION; INTERLEUKIN-2; OXIDATION-REDUCTION; PRECIPITATION; PROTEIN DENATURATION; PROTEINS; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; TEMPERATURE;

EID: 38749126403     PISSN: 09280987     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejps.2007.10.008     Document Type: Article

References (15)

1. Appel R.D., Bairoch A., and Hochstrasser D.F. A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server. Trends Biochem. Sci. 19 (1994) 258-260
2. Baek W.O., and Vijayalakshmi M.A. Effect of chemical glycosylation of RNase A on the protein stability and surface histidines accessibility in immobilized metal ion affinity electrophoresis (IMAGE) system. Biochim. Biophys. Acta 1336 (1997) 394-402
3. Clark E.D.B. Refolding of recombinant proteins. Curr. Opin. Biotechnol. 9 (1998) 157-163
4. Dill K.A., Alonso D.O., and Hutchinson K. Thermal stabilities of globular proteins. Biochemistry 28 (1989) 5439-5449
5. Eriksen S.H., Jensen B., and Olsen J. Effect of N-linked glycosylation on secretion, activity, and stability of alpha-amylase from Aspergillus oryzae. Curr. Microbiol. 37 (1998) 117-122
6. Fink A.L. Protein aggregation: folding aggregates, inclusion bodies and amyloid. Fold Des. 3 (1998) R9-R23
7. Gupta P., Hall C.K., and Voegler A.C. Effect of denaturant and protein concentrations upon protein refolding and aggregation: a simple lattice model. Protein Sci. 7 (1998) 2642-2652
8. Ha E., Wang W., and Wang Y.J. Peroxide formation in polysorbate 80 and protein stability. J. Pharm. Sci. 91 (2002) 2252-2264
9. Hiraiwa M., Soeda S., Martin B.M., Fluharty A.L., Hirabayashi Y., O'Brien J.S., and Kishimoto Y. The effect of carbohydrate removal on stability and activity of saposin B. Arch. Biochem. Biophys. 303 (1993) 326-331
10. Kwon K.S., and Yu M.H. Effect of glycosylation on the stability of alpha1-antitrypsin toward urea denaturation and thermal deactivation. Biochim. Biophys. Acta 1335 (1997) 265-272
11. Langlois M.R., Delanghe J.R., De Buyzere M.L., and Leroux-Roels G.G. Glycation of human tissue and serum creatine kinase. Clin. Chim. Acta 211 (1992) 83-92
12. Liu D.T. Glycoprotein pharmaceuticals: scientific and regulatory considerations, and the US Orphan Drug Act. Trends Biotechnol. 10 (1992) 114-120
13. Remmele Jr. R.L., Nightlinger N.S., Srinivasan S., and Gombotz W.R. Interleukin-1 receptor (IL-1R) liquid formulation development using differential scanning calorimetry. Pharm. Res. 15 (1998) 200-208
14. Rose M.C., Voter W.A., Sage H., Brown C.F., and Kaufman B. Effects of deglycosylation on the architecture of ovine submaxillary mucin glycoprotein. J. Biol. Chem. 259 (1984) 3167-3172
15. Tams J.W., and Welinder K.G. Glycosylation and thermodynamic versus kinetic stability of horseradish peroxidase. FEBS Lett. 421 (1998) 234-236