Site-directed removal of N-glycosylation sites in human gastric lipase

European Journal of Biochemistry

Volumn 262, Issue 3, 1999, Pages 644-651

  Wicker Planquart, Catherine ^a,b   Canaan, Stéphane ^a   Rivière, Mireille ^a   Dupuis, Liliane ^a  

^a Centre National de la Recherche Scientifique   (France)

^b CEA GRENOBLE   (France)

Author keywords

Baculovirus insect cell system; Gastric lipases; N glycosylation

Indexed keywords

ASPARAGINE; COMPLEMENTARY DNA; GLYCAN; OLIGOSACCHARIDE; PEPSIN A; SERINE; THREONINE; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME STABILITY; ENZYME SYNTHESIS; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STOMACH;

ANIMALS; BACULOVIRIDAE; CELL LINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HUMANS; HYDROLYSIS; LECTINS; LIPASE; MUTAGENESIS, SITE-DIRECTED; PEPSIN A; PROTEIN BINDING; SODIUM DODECYL SULFATE; SPODOPTERA; STOMACH; TRANSFECTION; TRIGLYCERIDES; TUNICAMYCIN;

EID: 0033564008     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00427.x     Document Type: Article

References (28)

1. 1. Bodmer, M.W., Angal, S., Yarranton, G.T., Harris, T.J.R., Lyons, A., King, D.J., Piéroni, G., Rivière, C., Verger, R. & Lowe, P.A. (1987) Molecular cloning of a human gastric lipase and expression of the enzyme in yeast. Biochim. Biophys. Acta 909, 237-244.
2. 2. Moreau, H., Abergel, C., Carrière, F., Ferrato, F., Fontecilla-Camps, J.C., Cambillau, C. & Verger, R. (1992) Isoform purification of gastric lipases. Towards crystallization. J. Mul. Biol. 225, 147-153.
3. 3. Wicker-Planquart, C., Canaan, S., Rivière, M., Dupuis, L. & Verger, R. (1996) Expression in insect cells and purification of a catalytically active recombinant human gastric lipase. Protein Eng. 9, 1225-1232.
4. 4. Canaan, S., Dupuis, L., Rivière, M., Belach, A., Verger, R., Remette, J.L. & Wicker-Planquart, C. (1998) In Production and Potential Applications of Recombinant Gastric Lipases in Biotechnology, Protein Engineering for Industrial Biotechnology (Alberghina, L., ed.). Harwood Academic Publishers, Amsterdam, in press.
5. 5. Sheriff, S., Du, H. & Grabowski, G.A. (1995) Characterization of lysosomal acid lipase by site-directed mutagenesis and heterologous expression. J. Biol. Chem. 270, 27766-27772.
6. 6. Kuroda, K., Geyer, H., Geyer, R., Doerfler, W. & Klenk, H.D. (1990) The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector. Virology 174, 418-429.
7. 7. Aeed, P.A. & Elhammer, A.P. (1994) Glycosylation of recombinant prorenin in insect cells: the insect cell line SF9 does not express the mannose 6-phosphate recognition signal. Biochemistry 33, 8793-8797.
8. 8. Kretzchmar, E., Geyer, R. & Klenk, H.D. (1994) Baculovirus infection does not alter N-glycosylation in Sodoptera frugiperda cells. Biol. Chem. Hoppe Seyler 375, 23-27.
9. 9. Lopez, M., Coddeville, B., Langridge, J., Plancke, Y., Sautiere, P., Chaabihi, H., Chirat, F., Harduin-Lepers, A., Cerutti, M., Verbert, A. & Delannoy, P. (1997) Microheterogeneity of the oligosaccharides carried by the recombinant bovine lactoferrin expressed in Mamestre brassicae cells. Glycobiology 7, 635-651.
10. 10. Moore, J.P., McKeating, J.A., Jones, I.M., Stephens, P.E., Clements, G., Thomson, S. & Weiss, R.A. (1990) Characterisation of recombinant gp120 and gp160 from HIV-1: binding to monoclonal antibodies and soluble CD4. AIDS 4, 307-315.
11. 11. Berg-Fussman, A., Grace, M.E., Ioannou, Y. & Grabowski, G.A. (1993) Human acid β-glucosidase. N-glycosylation site occupancy and the effect of glycosylation on enzymatic activity. J. Biol. Chem. 268, 14861-14866.
12. 12. Canaan, S., Dupuis, L., Rivière, M., Faessel, K., Romette, J.L., Verger, R. & Wicker-Planquart, C. (1998) Purification and interfacial behaviour of recombinant human gastric lipase produced from insect cells in a bioreactor. Protein Expr. Purif. 14, 23-30.
13. 13. Ivanovo, M.G., Aoubala, M., de Caro, A., Daniel, C., Hirn, J. & Verger, R. (1993) A study on human gastric lipase and complexes with monoclonal antibodies using the monomolecular film technique. Colloids Surf. B 1, 17-22.
14. 14. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
15. 15. Gershoni, J.M. & Palade, G.E. (1982) Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. Anal. Biochem. 124, 396-405.
16. 16. Gargouri, Y., Piéroni, G., Rivière, C., Saunière, J.-F., Lowe, P.A., Sarda, L. & Verger, R. (1986) Kinetic assay of human gastric lipase on short-and long-chain triacylglycerol emulsions. Gastroenterology 91, 919-925.
17. 17. Will, P.C., Allbee, W.E. & Witt, C.G. (1984) Quantification of pepsin A activity in canine and rat gastric juice with the chromogenic substrate azocoll. Clin. Chem. 30, 707-711.
18. 18. Butters, T.D., Hughes, R.C. & Vischer, P. (1981) Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin. Biochim. Biophys. Acta 640, 672-686.
19. 19. Hsieh, P. & Robbins, PW. (1984) Regulation of asparagine-linked oligosaccharides processing. J. Biol. Chem. 259, 2375-2382.
20. 20. Aoubala, M., Bonicel, J., Benicourt, C., Verger, R. & de Caro, A. (1994) Tryptic cleavage of gastric lipases: location of the single disulfide bridge. Biochem. Biophys. Acta 1213, 319-324.
21. 21. Gargouri, Y., Moreau, H. & Verger, R. (1989) Gastric lipases: biochemical and physiological studies. Biochim. Biophys. Acta 1006, 255-271.
22. 22. de la Fournière, L., Ivanovo, M.G., Blond, J.-P., Carrière, F. & Verger, R. (1994) Surface behaviour of human pancreatic and gastric lipases. Colloids Surf. B 2, 585-593.
23. 23. Picard, V., Ersdal-Badju, E. & Bock, S.C. (1995) Partial glycosylation of antithrombin III asparagine-135 is caused by the serine in the third position of its N-glycosylation consensus sequence and is responsible for production of the beta-antithrombin III isoform with enhanced heparin affinity. Biochemistry 34, 8433-8440.
24. 24. Fountoulakis, M. (1996) Apparent heterogeneity of recombinant interferon gamma receptors produced in prokaryotic and eukaryotic expression systems. J. Chem. Technol. Biotechnol. 65, 123-130.
25. 25. Lodish, H.F., Kong, N., Snider, M. & Strous, G.J. (1983) Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates. Nature 304, 80-83.
26. 26. Lodish, H.F. & Kong, N. (1983) Reversible block in intracellular transport and budding of mutant vesicular stomatitis glycoproteins. Virology 125, 335-348.
27. 27. Klassen, C.H., Fransen, J.A., Swarts, H.G. & De Pont, J.J. (1997) Glycosylation is essential for biosynthesis of functional gastric H+,K+-ATPase in insect cells. Biochem. J. 321, 419-424.
28. 28. Moreau, H., Bemadac, A., Gargouri, Y., Pieroni, G. & Verger, R. (1989) Immunocytolocalization of human gastric lipase in chief cells of the fundic mucosa. Histochemistry 91, 419-423.