Protein analysis based on molecular beacon probes and biofunctionalized nanoparticles

Science China Chemistry

Volumn 53, Issue 4, 2010, Pages 704-719

  Shi, Hui ^a   Yang, Xiao Hai ^a   He, Xiao Xiao ^a   Wang, Ke Min ^a   Wang, Qing ^a   Guo, Qiu Ping ^a   Huo, Xi Qin ^a  

^a Biomedical Engineering Center   (China)

Author keywords

Aptamer; Molecular beacon; Protein analysis; Protein DNA interaction; Proteomics; Silica nanoparticles

Indexed keywords

APTAMERS; MOLECULAR BEACON; PROTEIN ANALYSIS; PROTEOMICS; SILICA NANOPARTICLES;

CELL MEMBRANES; CHEMICAL DETECTION; MOLECULAR BIOLOGY; NANOPARTICLES; PROBES; SILICA;

PROTEINS;

EID: 77953319951     PISSN: 16747291     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11426-010-0110-3     Document Type: Review

References (138)

1. Dive C, Hickman JA. Drug-target interactions: only the first step in the commitment to a programmed cell death? British J cancer, 1991, 64(1): 192-196
2. Lennon SV, Martin SJ, Cotter TG. Dose-dependent induction of apoptosis in human tumour cell lines by widely diverging stimuli. Cell proliferation, 1991, 24(2): 203-214
3. Mathews DJ, Kopczynski J. Using model-system genetics for drug-based target discovery. Drug Discovery Today, 2001, 6(3): 141-149 (Pubitemid 32098288)
4. Fields S. PROTEOMICS: proteomics in genomeland. Science, 2001, 291(5507): 1221-1224 (Pubitemid 32173077)
5. Abbott A. And now for the proteome. Nature, 2001, 409(6822): 747-748
6. Voller A, Bartlett A, Bidwell DE. Enzyme immunoassays with special reference to ELISA techniques. J Clin Path, 1978, 31(6): 507-520 (Pubitemid 8358776)
7. Dalmau J, Furneaux HM, Gralla RJ, Kris MG, Posner JB. Detection of anti-Hu antibody in the serum of patients with small cell drug cancer-a quantitative western blot analysis. Ann Neurol, 1990, 27(5): 544-552 (Pubitemid 20149976)
8. Matkowskyj KA, Cox R, Jensen RT, Benya RV. Quantitative immunohistochemistry by measuring cumulative signal strength accurately measures receptor number. J Histochem Cytochem, 2003, 51(2): 205-214 (Pubitemid 36160047)
9. Alban A, David SO, Bjorkesten L, Andersson C, Sloge E, Lewis S, Currie I. A novel experimental design for comparative two-dimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics, 2003, 3(1): 36-44 (Pubitemid 36151066)
10. Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. Electrospray ionization for mass spectrometry of large biomolecules. Science, 1989, 246(4926): 64-71 (Pubitemid 19252085)
11. Oda Y, Huang K, Cross FR, Cowburn D, Chait BT. Accurate quantitation of protein expression and site-specific phosphorylation. Proc Natl Acad Sci USA, 1999, 96: 6591-6596 (Pubitemid 29274927)
12. Gygi SP, Rist B, Gerber SA, Turecek F, Gelb MH, Aebersold R. Quantitative analysis of complex protein mixtures using isotope- coded affinity tags. Nature Biotechnol, 1999, 17(10): 994-999 (Pubitemid 29474856)
13. Abbott A. A post-genomic challenge: learning to read patterns of protein synthesis. Nature, 1999, 402(6763): 715-720
14. Fang X, Li JJ, Perlette J, Tan W, Wang K. Molecular beacons: novel fluorescent probes. Anal Chem, 2000, 72(23): 747A-753A (Pubitemid 32002324)
15. Li J, Tang Z, Wang K, Tan W. Real-time protein monitoring based on molecular beacons. Current Proteomics, 2004, 1: 315-324 (Pubitemid 34687324)
16. Wang K, Tang Z, Yang CJ, Kim Y, Fang X, Li W, Wu Y, Medley CD, Cao Z, Li J, Colon P, Lin H, Tan W. Molecular engineering of DNA: molecular beacons. Angew Chem Int Ed, 2009, 48(5): 856-870
17. Tan W, Wang K, He X, Zhao X, Drake T, Wang L, Bagwe RP. Bionanotechnology based on silica nanoparticles. Med Res Rev, 2004, 24(5): 621-638 (Pubitemid 39038230)
18. Wang L, Wang K, Santra S, Zhao X, Hilliard LR, Smith JE, Wu Y, Tan W. Watching silica nanoparticles glow in the biological world. Anal Chem, 2006, 78(3): 646-654 (Pubitemid 43228570)
19. Yan J, Estévez MC, Smith JE, Wang K, He X, Wang L, Tan W. Dye-doped nanoparticles for bioanalysis. Nanotoday, 2007, 2(3): 44-50 (Pubitemid 46695432)
20. Liu JB, Yang XH, Wang KM, Wang D, Zhang PF. Chemical etching with tetrafluoroborate: a facile method for resizing of CdTe nanocrystals under mild conditions. Chem Commun, 2009, 40: 6080-6082
21. Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol, 1996, 14(3): 303-308 (Pubitemid 26149073)
22. Tyagi S, Marras SAE, Kramer FR. Wavelength-shifting molecular beacons. Nat Biotechnol, 2000, 18(11): 1191-1196
23. Tyagi S, Bratu DP, Kramer FR. Multicolor molecular beacons for allele discrimination. Nat Biotechnol, 1998, 16(1):49-53 (Pubitemid 28035948)
24. Zhang P, Beck T, Tan W. Design of a molecular beacon DNA probe with two fluorophores. Angew Chem Int Ed, 2001, 40(2): 402-405 (Pubitemid 32117660)
25. Guo Q, Yang X, Wang K, Tan W, Li W, Tang H, Li H. Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction. Nucleic Acids Res, 2009, 37(3): e20
26. Yang X, Zhao X, Zuo X, Wang K, Wen J, Zhang H. Nucleic acids detection using cationic fluorescent polymer based on one-dimensional microfluidic beads array. Talanta, 2009, 77(3): 1027-1031
27. Zuo X, Yang X, Wang K, Tan W, Li H, Zhou L, Wen J, Zhang H. Improving the performance of immobilized molecular beacons through cleavage. Anal Chim Acta, 2006, 567(2): 173-178 (Pubitemid 43732409)
28. Tang H, Yang X, Wang K, Tan W, Li W. mRNA detection in living cell using phosphorothioate-modified molecular beacon. Chin Sci Bull, 2009, 54(9): 1507-1514
29. Tang H, Yang X, Wang K, Tan W, Liu B, He L, Wang W. Monitoring p21 mRNA expression in living cell based on molecular beacon fluorescence increasing rate. Chin Sci Bull, 2007, 53(3): 357-361 (Pubitemid 351236778)
30. Liu B, Wang K, Xiao Z, Wang W, Tan W, Sun Y, Tang H, Yang X. Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon. Chin Sci Bull, 2006, 51(17): 2059-2064 (Pubitemid 44394617)
31. Bratu DP, Cha BJ, Mhlanga MM, Kramer FR, Tyagi S. Visualizing the distribution and transport of mRNAs in living cells. Proc Natl Acad Sci USA, 2003, 100(23): 13308-13313 (Pubitemid 37444738)
32. Perlette J, Tan W. Real-time monitoring of intracellular mRNA hybridization inside single living cells. Anal Chem, 2001, 73(22): 5544-5550 (Pubitemid 33108566)
33. Medley CD, Drake TJ, Tomasini JM, Rogers RJ, Tan W. Simultaneous monitoring of the expression of multiple genes inside of single breast carcinoma cells. Anal Chem, 2005, 77(15): 4713-4718 (Pubitemid 41076658)
34. Santangelo PJ, Nix B, Tsourkas A, Bao G. Dual FRET molecular beacons for mRNA detection in living cells. Nucleic Acids Res, 2004, 32(6): e57
35. Meng X, Wang K, Tan W, Li J, Tang Z, Guo Q, Huang S, Li D. Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process. Chin Sci Bull, 2003, 48(23): 2581-2584 (Pubitemid 44553019)
36. Li W, Yang X, Wang K, Tan W, He Y, Guo Q, Tang H, Liu J. Real-time imaging of protein internalization using aptamer conjugates. Anal Chem, 2008, 80(13): 5002-5008 (Pubitemid 351956308)
37. Li JJ, Geyer R, Tan W. Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA. Nucleic Acids Res, 2008, 28(11): e52
38. Tang Z, Wang K, Tan W, Li J, Liu L, Guo Q, Meng X, Ma C, Huang S. Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons. Nucleic Acids Res, 2003, 31(23): e148
39. Tang Z, Wang K, Tan W, Ma C, Li J, Liu L, Guo Q, Meng X. Real-time investigation of nucleic acide phosphorylation process using molecular beacons. Nucleic Acids Res, 2005, 33(11): e97 (Pubitemid 41430592)
40. Ma C, Tang Z, Wang K, Tan W, Li J, Li W, Li Z, Yang X, Li H, Liu L. Real-time monitoring of DNA polymerase activity using molecular beacon. Anal Biochem, 2006, 353(1): 141-143
41. Ma C, Tang Z, Wang K, Tan W, Yang X, Li W, Li Z, Lv X. Real-time monitoring of restriction endonuclease activity using molecular beacon. Anal Biochem, 2007, 363(2): 294-296 (Pubitemid 46437317)
42. Ma C, Tang Z, Wang K, Tan W, Yang X, Li W, Li Z, Li H, Lv X. Real-time monitoring of nucleic acid dephosphorylation by using molecular beacons. Chem Bio Chem, 2007, 8(13): 1487-1490 (Pubitemid 47436902)
43. Li J, Yan H, Wang K, Tan W, Zhou X. Hairpin fluorescence DNA probe for real-time monitoring of DNA methylation. Anal Chem, 2007, 79(26): 1050-1056 (Pubitemid 46254405)
44. Liu B, Yang X, Wang K, Tan W, Li H, Tang H. Real-time monitoring of uracil removal by uracil-DNA glycosylase using fluorescent resonance energy transfer probes. Anal Biochem, 2007, 366(2): 237-243 (Pubitemid 46908717)
45. Biggins JB, Prudent JR, Marshall DJ, Ruppen M, Thorson JS. A continuous assay for DNA cleavage: The application of "break lights" to enediynes, iron-dependent agents, and nucleases. Proc Natl Acad Sci USA, 2000, 97(25): 13537-13542
46. Fang X, Liu X, Schuster S, Tan W. Designing a novel molecular beacon for surface-immobilized DNA hybridization studies. J Am Chem Soc, 1999, 121(12): 2921-2922 (Pubitemid 29165532)
47. Li W, Yang X, Wang K, Tan W, Li H, Ma C. FRET-based aptamer probe for rapid angiogenin detection. Talanta, 2008, 75(3): 770-774
48. Li W, Wang K, Tan W, Ma C, Yang X. Aptamer-based analysis of angiogenin by fluorescence anisotropy. Analyst, 2007, 132(2): 107-113 (Pubitemid 46169507)
49. Yang X, Wang L, Wang K, Tan W, Tang H, Meng X, Guo Q. Novel protein detection method based on proximity-dependent polymerase reaction and aptamers. Chin Sci Bull, 2007, 53(2): 204-208 (Pubitemid 351230530)
50. Yang X, Wang S, Wang K, Luo X, Tan W, Cui L. Novel method of protein detection based on cationic conjugated polymer and aptamer probe. Chem J Chin Univ, 2009, 30(5): 899-902
51. Fang X, Li JJ, Tan W. Using molecular beacons to probe molecular interactions between lactate dehydrogenase and single-stranded DNA. Anal Chem, 2000, 72(14): 3280-3285 (Pubitemid 30482504)
52. Li JJ, Fang X, Schuster SM, Tan W. Molecular beacons: a novel approach to detect protein-DNA interactions. Angew Chem Int Ed, 2000, 39(6): 1049-1052 (Pubitemid 30217241)
53. Tan W, Fang X, Li J, Liu X. Molecular beacons: a novel DNA probe for nucleic acid and protein studies. Chem Eur J, 2000, 6(7): 1107-1111 (Pubitemid 30586031)
54. He X, Jin R, Yang L, Wang K, Li W, Tan W, Li H. Study on the specific interaction between angiogenin and aptamer by atomic force microscopy (AFM). Chin Sci Bull, 2008, 53(2): 198-203 (Pubitemid 351230532)
55. Ma C, Tang Z, Huo X, Yang X, Li W, Tan W. Real-time monitoring of double-stranded DNA cleavage using molecular beacons. Talanta, 2008, 76(2): 458-461
56. Liu L, Tang Z, Wang K, Tan W, Li J, Guo Q, Meng X, Ma C. Using molecular beacon to monitor activity of E. coli DNA ligase. Analyst, 2005, 130(3): 350-357 (Pubitemid 40388938)
57. Tang Z, Wang K ,Tan W, Li J, Liu L, Guo Q, Meng X, Huang S, Li D, Luo W. Real time monitoring of nucleic acids ligation based on molecular beacon. Chin Sci Bull, 2003, 48(3): 1215-1218 (Pubitemid 44553084)
58. Yao G, Tan W. Molecular-beacon-based array for sensitive DNA analysis. Anal Biochem, 2004, 331(2): 216-223 (Pubitemid 38953070)
59. Wang H, Li J, Liu H, Liu Q, Mei Q, Wang Y, Zhu J, He N, Lu Z. Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film. Nucleic Acids Res, 2002, 30(12): e61
60. Fang X, Tan W. Imaging single fluorescent molecules at the interface of an optical fiber probe by evanescent wave excitation. Anal Chem, 1999, 71(15): 3101-3105 (Pubitemid 29372724)
61. Bonnet G, Libchaber A, Kramer FR, Tyagi S. Thermodynamic basis of the enhanced specificity of structured DNA probes. Proc Natl Acad Sci USA, 1999, 96(11): 6171-6176 (Pubitemid 29256638)
62. Li JJ, Fang X, Schuster SM, Tan W. Molecular beacons: a novel approach to detect protein-DNA interactions. Angew Chem Int Ed, 2000, 112(6), 1091-1094 (Pubitemid 30217241)
63. Tuerk C, Gold L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science, 1990, 249(4968): 505-510 (Pubitemid 20248891)
64. Jayasena SD. Aptamers: An emerging class of molecules that rival antibodies in diagnostics. Clin Chem, 1999, 45(9): 1628-1650
65. Li JJ, Fang X, Tan W. Molecular aptamer beacons for real-time protein recognition. Biochem Bioph Res Co, 2002, 292(1): 31-40 (Pubitemid 34687324)
66. Harper JW, Fox EA, Shapiro R, Vallee BL. Mutagenesis of residues flanking Lys-40 enhances the enzymatic activity and reduces the angiogenic potency of angiogenin. Biochemistry, 1990, 29(31): 7297-7302 (Pubitemid 20230060)
67. Yoshioka N, Wang L, Kishimoto K, Tsuji T, Hu GF. A therapeutic target for prostate cancer based on angiogenin-stimulated angiogenesis and cancer cell proliferation. Proc Natl Acad Sci USA, 2006, 103: 14519-14524 (Pubitemid 44484782)
68. Fang X, Cao Z, Beck T, Tan W. Molecular aptamer for real-time oncoprotein platelet-derived growth factor monitoring by fluorescence anisotropy. Anal Chem, 2001, 73(23): 5752-5757 (Pubitemid 33126734)
69. Lou H, Brister JR, Li JJ, Chen W, Muzyczka N, Tan W. Adeno-associated virus Rep78/Rep68 promotes localized melting of the Rep binding element in the absence of ATP. ChemBioChem, 2004, 5(3): 324-332 (Pubitemid 39257125)
70. Lang P, Yeow K, Nichols A, Scheer A. Cellular imaging in drug discovery. Nat Rev Drug Discovery, 2006, 5: 343-356
71. Ruan G, Agrawal A, Marcus AI, Nie S. Imaging and tracking tat-peptide conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding. J Am Chem Soc, 2007, 129: 14759-14766 (Pubitemid 350207890)
72. Hu G, Xu C, Riordan JF. Human angiogenin is rapidly translocated to the nucleus of human umbilical vein endothelial cells and binds to DNA. J Cell Biochem, 2000, 76: 452-462 (Pubitemid 30078454)
73. Li R, Riordan JF, Hu G. Nuclear translocation of human angiogenin in cultured human umbilical artery endothelial cells is microtubule and lysosome independent. Biochem Biophys Res Commun, 1997, 238(2), 305-312 (Pubitemid 27464376)
74. Doherty AJ, Dafforn TR. Nick recognition by DNA ligases. J Mol Biol, 2000, 296: 43-56
75. Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res, 1995, 23: 4407-4414
76. Kinoshita Y, Nishigaki K, Husimi Y. Fluorescence-, isotope- or biotin- labeling of the 5'-end of single-stranded DNA/RNA using T4 RNA ligase. Nucleic Acids Res, 1997, 25: 47-48 (Pubitemid 27390199)
77. Wang LK, Lima CD, Shuman S. Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme. EMBO J, 2002, 21: 3873-3880 (Pubitemid 34787059)
78. Whitehouse J, Taylor RM, Thistlethwaite A, Zhang H, Karimi- Busheri F, Lasko DD, Weinfeld M, Caldecott KW. XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair. Cell, 2001, 104: 107-117 (Pubitemid 32144976)
79. Pingoud A, Jeltsch A. Structure and function of type II restriction endonucleases. Nucleic Acids Res, 2001, 29(18): 3705-3727 (Pubitemid 32910514)
80. Tan W, Shi Z, Smith S, Birnbaum D, Kopelman R. Submicrometer intracellular chemical optical fiber sensors. Science, 1992, 258(5083): 778-781
81. Bratten CDT, Cobbold PH, Cooper JM. Single-cell measurements of purine release using a micromachined electroanalytical sensor. Anal Chem, 1998, 70(6): 1164-1170 (Pubitemid 128500680)
82. Kneuer C, Sameti M, Bakowsky U, Schiestel T, Schirra H, Schmidt H, Lehr CM. A nonviral DNA delivery system based on surface modified silica-nanoparticles can efficiently transfect cells in vitro. Bioconjug Chem, 2000, 11(6): 926-932
83. Kneuer C, Sameti M, Haltner EG, Schiestel T, Schirra H, Schmidt H, Lehr CM. Silica nanoparticles modified with aminosilanes as carriers for plasmid DNA. Int J Pharm, 2000, 196(2): 257-261 (Pubitemid 30105756)
84. Mao H, Roy K, Troung-Le VL, Janes KA, Lin KY, Wang Y, August JT, Leong KW. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization, and transfection efficiency. J Control Release, 2001, 70(3): 399-421 (Pubitemid 32155463)
85. Benns JM, Kim SW. Tailoring new gene delivery designs for specific targets. J Drug Target, 2000, 8(1): 1-12
86. Truong-Le VL, Walsh SM, Schweibert E, Mao H, Guggino WB, August JT, Leong KM. Gene transfer by DNA-gelatin nanospheres. Arch Biochem Biophys, 1999, 361(1): 47-56 (Pubitemid 29393713)
87. Soppimath KS, Aminabhavi TM, Kulkarni AR, Rudzinski WE. Biodegradable polymeric nanoparticles as drug delivery devices. J Control Release, 2001, 70: 1-20 (Pubitemid 32144788)
88. Sershen SR, Westcott SL, Halas NJ, West JL. Temperature-sensitive polymer-nanoshell composites for photothermally modulated drug delivery. J Biomed Mater Res, 2000, 51(3): 293-298 (Pubitemid 30452130)
89. Kubitschko S, Spinke J, Bruckner T, Pohl S, Oranth N. Sensitivity enhancement of optical immunosensors with nanoparticles. Anal Biochem, 1997, 253(1): 112-122 (Pubitemid 27490612)
90. Lyon LA, Musick MD, Smith PC, Reiss BD, Pena DJ, Natan MJ. Surface plasmon resonance of colloidal Au-modified gold films. Sensor Actuat B-Chem, 1999, 54:118-124
91. Shaertl S, Meyer-Almes FJ, Lopez-Calle E, Siemers A, Kramer J. A novel and robust homogeneous fluorescence-based assay using nanoparticles for pharmaceutical screening and diagnostics. J Biomol Screen, 2000, 5(4): 227-237
92. Santra S, Zhang P, Wang K, Tapec R, Tan W. Conjugation of biomolecules with luminophore-doped silica nanoparticles for photostable biomarkers. Anal Chem, 2001, 73(20): 4988-4993 (Pubitemid 32980123)
93. He X, Wang K, Tan W, Xiao D, Li J, Yang X. A novel fluorescent label based on biological fluorescent nanoparticles and its application in cell recognition. Chin Sci Bull, 2001, 46(23): 1962-1965 (Pubitemid 34032417)
94. He X, Wang K, Tan W, Li J, Yang X, Huang S, Li D, Xiao D. Photostable luminescent nanoparticles as biological label for cell recognition of system lupus erythematosus patients. J Nanosci Nanotech, 2002, 2: 317-320 (Pubitemid 135714140)
95. He X, Duan J, Wang K, Tan W, Lin X, He C. A novel fluorescent label based on organic dye-doped silica nanoparticles for HepG liver cancer cell recognition. J Nanosci Nanotech, 2004, 4(6): 585-589
96. Peng J, Wang K, Tan W, He X, He C, Wu P, Liu F. Identification of liver cancer cells in a mixed cell system using galactose-conjugated fluorescent nanoparticles. Talanta, 2007, 71(2): 833-840 (Pubitemid 46108960)
97. He X, Chen J, Wang K, Qin D, Tan W. Preparation of luminescent Cy5 doped core-shell SFNPs and its application as a near-infrared fluorescent marker. Talanta, 2007, 72(4): 1519-1526 (Pubitemid 46738573)
98. Wu P, He X, Wang K, Tan W, Ma D, Yang W, He C. Imaging breast cancer cells and tissues using peptide-labeled fluorescent silica nanoparticles. J Nanosci Nanotech, 2008, 8(5): 2483-2487
99. He X, Ge J, Wang K, Tan W, Shi H, He C. FSiNPs mediated improved double immunofluorescence staining for gastric cancer cells imaging. Talanta, 2008, 76(5): 1199-1206
100. Shi H, He X, Wang K, Yuan Y, Deng K, Chen J, Tan W. Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)-based bioprobes conjugated to Annexin V for apoptosis detection and imaging. Nanomedicine: Nanotechnology, Biology, and Medicine, 2007, 3(4): 266-272 (Pubitemid 350199642)
101. Li Z, Wang K, Tan W, Li J, Fu Z, Ma C, Li H, He X, Liu J. Immunofluorescent labeling of cancer cells with quantum dots synthesized in aqueous solution. Anal Biochem, 2006, 354(2): 169-174 (Pubitemid 43949240)
102. Li Z, Wang K, Tan W, Li J, Fu Z, Wang Y, Liu J, Yang X. Preparation of luminescent CdTe quantum dots doped core-shell nanoparticles and their application in cell recognition. Chin Sci Bull, 2005, 50(16): 1703-1707 (Pubitemid 44543430)
103. Qin D, He X, Wang K, Zhao X, Tan W, Chen J. Fluorescent nanoparticle-based indirect immunofluorescence microscopy for detection of Mycobacterium tuberculosis. J Biomed Biotechnol, 2007, ID: 89364 (Pubitemid 350243168)
104. Qin D, He X, Wang K, Tan W. Using fluorescent nanoparticles and SYBR Green I based two-color flow cytometry to determine Mycobacterium tuberculosis avoiding false positives. Biosens Bioelectron, 2008, 24(4): 626-631
105. He X, Wang Y, Wang K, Peng J, Liu F, Tan W. Research of the relationship of intracellular acidification and apoptosis in Hela cells based on pH nanosensors. Sci China Ser B-Chem, 2007, 5(2): 258-265 (Pubitemid 47110158)
106. Peng J, He X, Wang K, Tan W, Wang Y, Liu Y. Noninvasive monitoring of intracellular pH change induced by drug stimulation using silica nanoparticle sensors. Anal Bioanal Chem, 2007, 388(3): 645-654 (Pubitemid 46709849)
107. Zhao X, Tapec-Dytioco R, Wang K, Tan W. Collection of trace amounts of DNA/mRNA molecules using genomagnetic nanocapturers. Anal Chem, 2003, 75(14): 3476-3483 (Pubitemid 36871238)
108. Ashtari P, He X, Wang K, Gong P. An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles. Talanta, 2005, 67(3): 548-554 (Pubitemid 41240595)
109. He X, Huo H, Wang K, Tan W, Gong P, Ge J. Plasmind DNA isolation using amino-silica coated magnetc nanoparticles (ASMNPs). Talanta, 2007, 73(4): 764-769 (Pubitemid 47379773)
110. He X, Chen Y, Wang K, Wu P, Gong P, Huo H. Selective separation of proteins with pH-dependent magnetic nanoadsorbents. Nanotechnology, 2007, 18(36): 365604 (6pp) (Pubitemid 47413712)
111. Gong P, He X, Wang K, Tan W, Xie W, Wu P, Li H. Combination of functionalized nanoparticles and polymerase chain reaction-based method for SARS-CoV gene detection. J Nanosci Nanotech, 2008, 8(1): 293-300 (Pubitemid 351594261)
112. Peng J, Xing X, Wang K, Tan W, He X, Huang S. Influence of anions on the formation and properties of chitosan-DNA nanoparticles. J Nanosci Nanotechnol, 5(5): 713-717
113. Peng J, He X, Wang K, Tan W, Li H, Xing X, Wang Y. An antisense oligonucleotide carrier based on amino silica nanoparticles for antisense inhibition of cancer cells. Nanomedicine: Nanotechnology, Biology and Medicine, 2006, 2(2): 113-120 (Pubitemid 43956609)
114. 4@Ag nanoparticles. Nanotechnology, 2007, 18: 285604 (7pp) (Pubitemid 46290622)
115. He X, Wu X, Wang K, Shi B, Hai L. Methylene blue-encapsulated phosphonate-terminated silica nanoparticles for simultaneous in vivo imaging and photodynamic therapy. Biomaterials, 2009, 30(29): 5601-5609
116. Wu P, He X, Wang K, Tan W, He C, Zheng M. A novel methotrexate delivery system based on chitosan-methotrexate covalently conjugated nanoparticles. J Biomed Nanotechnol, 2009, 5(5): 557-564
117. He X, Wang K, Tan W, Liu B, Lin X, He C, Li D, Huang S, Li J. Bioconjugated nanoparticles for DNA protection from cleavage. J Am Chem Soc, 2003, 125(24): 7168-7169 (Pubitemid 36798952)
118. Zhao X, Tapec-Dytioco R, Tan W. Ultrasensitive DNA detection using highly fluorescent bioconjugated nanoparticles. J Am Chem Soc, 2003, 125(38): 11474-11475 (Pubitemid 37210079)
119. Zhao X, Hilliard LR, Mechery SJ, Wang Y, Bagwe RP, Jin S, Tan W. A rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles. Proc Natl Acad Sci USA, 2004, 101(42): 15027-15032 (Pubitemid 39391712)
120. Santra S, Wang K, Tapec R, Tan W. Development of novel dye-doped silica nanoparticles for biomarker application. J Biomed Opt, 2001, 6: 160-166 (Pubitemid 32546881)
121. He X, Wang K, Tan W, Chen J, Duan J, Yuan Y, Lin X. Study on the effect of electrostatic interaction on core-shell nanoparticles preparation with microemulsion technique. Chin Sci Bull, 2005, 50(24): 2821-2826 (Pubitemid 44552465)
122. Yamauchi H, Ishikawa T, Kondo S. Surface characterization of ultramicro spherical particles of silica prepared by w/o microemulsion method. Colloids Surf, 1989, 37: 71-80
123. Bagwe RP, Yang CY, Hilliard LR, Tan WH. Optimization of dye-doped silica nanoparticles prepared using a reverse microemulsion method. Langmuir, 2004, 20(19): 8336-8342
124. Zhao X, Bagwe RP, Tan W. Development of organic-dye-doped silica\ nanoparticles in reverse microemulsion. Adv Mater, 2004, 16: 173-176
125. Wang L, Yang C, Tan W. Dual-luminophore-doped silica nanoparticles for multiplexed signaling. Nano Lett, 2005, 5(1): 37-43 (Pubitemid 40168900)
126. Wang L, Tan W. Multicolor FRET silica nanoparticles by single wavelength excitation. Nano Lett, 2006, 6(1): 84-88 (Pubitemid 43166106)
127. Qhobosheane M, Santra S, Zhang P, Tan W. Biochemically functionalized silica nanoparticles. Analyst, 2001, 126: 1274-1278 (Pubitemid 32747743)
128. Tapec R, Zhao J, Tan W. Development of organic dye-doped silica nanoparticle for bioanalysis and biosensors. J Nanosci Nanotechnol, 2002, 2: 405 (Pubitemid 135714153)
129. Hilliard LR, Zhao X, Tan W. Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies. Anal Chim Acta, 470(1): 51-56
130. Bagwe RP, Milliard LR, Tan W. Surface modification of silica nanoparticles to reduce aggregation and nonspecific binding. Langmuir, 2006, 22(9): 4357-4382
131. Shi H, He X, Wang K, Yuan Y, Tan W. Bioeffects of silica nanoparticles and silica microparticles as carriers for enzyme immobilization. Chem J Chin Univ, 2007, 28(9): 1690-1695 (Pubitemid 350063158)
132. 2 nanoparticles. Exp Cell Res, 2005, 305: 51-62 (Pubitemid 40380653)
133. Xing X, He X, Peng J, Wang K, Tan W. Uptake of silica-coated nanoparticles by HeLa cells. J Nanosci Nanotechnol, 2005, 5(10): 1688-1693 (Pubitemid 44651977)
134. He X, Liu F, Wang K, Ge J, Qin D, Gong P, Tan W. Bioeffects of different functionalized silica nanoparticles on HaCaT cell line. Chin Sci Bull, 2006, 51(16): 1939-1946 (Pubitemid 44432133)
135. He X, Nie H, Wang K, Tan W, Wu X, Zhang P. In vivo study of biodistribution and urinary excretion of surface-modified silica nanoparticles. Anal Chem, 2008, 80 (24): 9597-9603
136. Heemels MT. Apoptosis. Nature, 2000, 407:769
137. Fadok VA, de Cathelineau A, Daleke DL, Henson PM, Bratton DL. Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblasts. J Biol Chem, 2001, 276: 1071-1077 (Pubitemid 32096530)
138. Gac SL, Vermes I, Berg A. Quantum dots based probes conjugated to Annexin V for photostable apoptosis detection and imaging. Nano Lett, 2006, 6: 1863-1869 (Pubitemid 44555222)