Mutations in the lipid A deacylase PagL which release the enzyme from its latency affect the ability of PagL to interact with lipopolysaccharide in Salmonella enterica serovar Typhimurium

Biochemical and Biophysical Research Communications

Volumn 396, Issue 4, 2010, Pages 812-816

  Manabe, Takayuki ^a   Kawano, Miyuki ^a   Kawasaki, Kiyoshi ^a  

^a DOSHISHA WOMEN'S COLLEGE OF LIBERAL ARTS   (Japan)

Author keywords

Deacylation; Endotoxin; Gram negative bacteria; Membrane lipid; Outer membrane

Indexed keywords

BACTERIAL ENZYME; BACTERIUM LIPOPOLYSACCHARIDE; LIPID A DEACYLASE; MEMBRANE PROTEIN; MUTANT PROTEIN; PROTEIN PAGL; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL OUTER MEMBRANE; CONTROLLED STUDY; DEACYLATION; ENZYME ACTIVITY; ENZYME RELEASE; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN CARBOHYDRATE INTERACTION; SALMONELLA ENTERICA; SALMONELLA TYPHIMURIUM; SEROTYPE; WILD TYPE;

ACETYLATION; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CARBOXYLIC ESTER HYDROLASES; LIPOPOLYSACCHARIDES; MOLECULAR SEQUENCE DATA; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; SALMONELLA TYPHIMURIUM;

NEGIBACTERIA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

EID: 77953287586     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.04.153     Document Type: Article

References (18)

1. Nikaido H. Molecular basis of bacterial outer membrane permeability revisited. Microbiol. Mol. Biol. Rev. 67 (2003) 593-656
2. Park B.S., Song D.H., Kim H.M., Choi B.S., Lee H., and Lee J.O. The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex. Nature 458 (2009) 1191-1195
3. Guo L., Lim K.B., Gunn J.S., Bainbridge B., Darveau R.P., Hackett M., and Miller S.I. Regulation of lipid A modifications by Salmonella typhimurium virulence genes phoP-phoQ. Science 276 (1997) 250-253
4. Nummila K., Kilpelainen I., Zahringer U., Vaara M., and Helander I.M. Lipopolysaccharides of polymyxin B-resistant mutants of Escherichia coli are extensively substituted by 2-aminoethyl pyrophosphate and contain aminoarabinose in lipid A. Mol. Microbiol. 16 (1995) 271-278
5. Kawasaki K., Ernst R.K., and Miller S.I. 3-O-deacylation of lipid A by PagL, a PhoP/PhoQ-regulated deacylase of Salmonella typhimurium, modulates signaling through Toll-like receptor 4. J. Biol. Chem. 279 (2004) 20044-20048
6. Miller S.I., Kukral A.M., and Mekalanos J.J. A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence. Proc. Natl. Acad. Sci. USA 86 (1989) 5054-5058
7. Groisman E.A., Chiao E., Lipps C.J., and Heffron F. Salmonella typhimurium phoP virulence gene is a transcriptional regulator. Proc. Natl. Acad. Sci. USA 86 (1989) 7077-7081
8. Garcia Vescovi E., Soncini F.C., and Groisman E.A. Mg2+ as an extracellular signal: environmental regulation of Salmonella virulence. Cell 84 (1996) 165-174
9. Trent M.S., Pabich W., Raetz C.R., and Miller S.I. A PhoP/PhoQ-induced Lipase (PagL) that catalyzes 3-O-deacylation of lipid A precursors in membranes of Salmonella typhimurium. J. Biol. Chem. 276 (2001) 9083-9092
10. Bishop R.E., Gibbons H.S., Guina T., Trent M.S., Miller S.I., and Raetz C.R. Transfer of palmitate from phospholipids to lipid A in outer membranes of gram-negative bacteria. EMBO J. 19 (2000) 5071-5080
11. Kawasaki K., Ernst R.K., and Miller S.I. Inhibition of Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation by aminoarabinose membrane modification. J. Bacteriol. 187 (2005) 2448-2457
12. Manabe T., and Kawasaki K. Extracellular loops of lipid A 3-O-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonella enterica serovar typhimurium. J. Bacteriol. 190 (2008) 5597-5606
13. Jia W., El Zoeiby A., Petruzziello T.N., Jayabalasingham B., Seyedirashti S., and Bishop R.E. Lipid trafficking controls endotoxin acylation in outer membranes of Escherichia coli. J. Biol. Chem. 279 (2004) 44966-44975
14. Kawasaki K., China K., and Nishijima M. Release of the lipopolysaccharide deacylase PagL from latency compensates for a lack of lipopolysaccharide aminoarabinose modification-dependent resistance to the antimicrobial peptide polymyxin B in Salmonella enterica. J. Bacteriol. 189 (2007) 4911-4919
15. Brown E.A., and Hardwidge P.R. Biochemical characterization of the enterotoxigenic Escherichia coli LeoA protein. Microbiology 153 (2007) 3776-3784
16. Murray G.L., Attridge S.R., and Morona R. Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz. Mol. Microbiol. 47 (2003) 1395-1406
17. Rutten L., Geurtsen J., Lambert W., Smolenaers J.J., Bonvin A.M., de Haan A., van der Ley P., Egmond M.R., Gros P., and Tommassen J. Crystal structure and catalytic mechanism of the LPS 3-O-deacylase PagL from Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 103 (2006) 7071-7076
18. Geurtsen J., Steeghs L., Hove J.T., van der Ley P., and Tommassen J. Dissemination of lipid A deacylases (pagL) among gram-negative bacteria: identification of active-site histidine and serine residues. J. Biol. Chem. 280 (2005) 8248-8259