Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus- the role of conserved residues in the active site

FEBS Journal

Volumn 277, Issue 12, 2010, Pages 2683-2695

  Tsuji, Hiroaki ^a   Nishimura, Shigenori ^a   Inui, Takashi ^a   Kado, Yuji ^b   Ishikawa, Kazuhiko ^b   Nakamura, Tsutomu ^b   Uegaki, Koichi ^b  

^a Osaka Prefecture University   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Chitinase; Crystal structure; DXDXE motif; Glycoside hydrolase family; Pyrococcus furiosus

Indexed keywords

ASPARTIC ACID; CHITINASE; GLUTAMIC ACID; OLIGOSACCHARIDE;

ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DXDXE MOTIF; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; HYDROGEN BOND; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN MOTIF; PROTON TRANSPORT; PYROCOCCUS FURIOSUS;

EID: 77953183572     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2010.07685.x     Document Type: Article

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