Mutations of Trp275 and Trp397 altered the binding selectivity of Vibrio carchariae chitinase A

Biochimica et Biophysica Acta - General Subjects

Volumn 1770, Issue 8, 2007, Pages 1151-1160

  Suginta, Wipa ^a   Songsiriritthigul, Chomphunuch ^a,b   Kobdaj, Archara ^a   Opassiri, Rodjana ^a   Svasti, Jisnuson ^c  

^a SURANAREE UNIVERSITY OF TECHNOLOGY   (Thailand)

^b National Synchrotron Research Center Thailand   (Thailand)

^c MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Active site mutation; Chitin hydrolysis; Chitinase A; Chitooligosaccharide; Specific hydrolyzing activity; Thin layer chromatography; Vibrio carchariae

Indexed keywords

4 NITROPHENYL N,N' DIACETYL BETA DEXTRO CHITOBIOSIDE; CHITIN; CHITINASE; CHITOBIOSE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; BINDING ASSAY; CONTROLLED STUDY; ENZYME STRUCTURE; HYDROLYSIS; KINETICS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY; VIBRIO HARVEYI; WILD TYPE;

BINDING SITES; BIOLOGICAL ASSAY; CHITIN; CHITINASE; CHROMATOGRAPHY, THIN LAYER; CIRCULAR DICHROISM; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; TRYPTOPHAN; VIBRIO;

VIBRIO HARVEYI;

EID: 34347247289     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbagen.2007.03.012     Document Type: Article

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