Importance of exposed aromatic residues in Chitinase B from Serratia marcescens 2170 for crystalline chitin hydrolysis

Journal of Biochemistry

Volumn 136, Issue 2, 2004, Pages 163-168

  Katouno, Fuminori ^a   Taguchi, Masashi ^a   Sakurai, Kengo ^a   Uchiyama, Taku ^a   Nikaidou, Naoki ^a   Nonaka, Takamasa ^b   Sugiyama, Junji ^c   Watanabe, Takeshi ^a  

^a NIIGATA UNIVERSITY   (Japan)

^b NAGAOKA UNIVERSITY OF TECHNOLOGY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Aromatic amino acid residues; Chitinase; Crystalline chitin; Serratia marcescens; Site directed mutagenesis

Indexed keywords

CHITIN; CHITINASE; CHITINASE A; CHITINASE B; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING ASSAY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME ISOLATION; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; NONHUMAN; PROTEIN HYDROLYSIS; PROTEIN STRUCTURE; SERRATIA MARCESCENS; SITE DIRECTED MUTAGENESIS;

CATALYSIS; CHITIN; CHITINASE; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROLYSIS; MICROFIBRILS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHENYLALANINE; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SERRATIA MARCESCENS; TIME FACTORS; TRYPTOPHAN; TYROSINE;

BACTERIA (MICROORGANISMS); SERRATIA MARCESCENS;

EID: 4644292779     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh105     Document Type: Article

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