A real-time fluorescent assay of the purified nitric oxide receptor, guanylyl cyclase

Analytical Biochemistry

Volumn 402, Issue 2, 2010, Pages 129-136

  Newton, Michael ^a   Niewczas, Izabella ^a,b   Clark, Jonathan ^b   Bellamy, Tomas C ^a,c  

^a BABRAHAM INSTITUTE   (United Kingdom)

^b Babraham Bioscience Technologies Ltd   (United Kingdom)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

CGMP; Guanylyl cyclase; Nitric oxide; Real time assay

Indexed keywords

CHEMICAL ACTIVATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NITRIC OXIDE; NUCLEOTIDES; PROTEINS; PURIFICATION; SIGNAL TRANSDUCTION;

CGMP; FLUORESCENCE INTENSITIES; GUANYLYL CYCLASE; INTERCELLULAR SIGNALING; PHYSIOLOGICAL EFFECTS; REAL-TIME ASSAYS; SIGNAL TRANSDUCTION PATHWAYS; SOLUBLE GUANYLYL CYCLASE (SGC);

FLUORESCENCE;

1H 1,2,4 OXADIAZOLO[4,3 A]QUINOXALIN 1 ONE; 4 AMINO 5 CYCLOPROPYL 2 [1 (2 FLUOROBENZYL) 1H PYRAZOLO[3,4 B]PYRIDIN 3 YL]PYRIMIDINE; CYCLIC GMP; GUANYLATE CYCLASE; NITRIC OXIDE;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYCLIZATION; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME MODIFICATION; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICHAELIS MENTEN KINETICS; PHARMACOLOGICAL PROCEDURES; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SEPARATION TECHNIQUE;

EID: 77953083424     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.03.041     Document Type: Article

References (31)

1. Moncada S., Palmer R.M., Higgs E.A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 1991, 43:109-142.
2. Garthwaite J. Concepts of neural nitric oxide-mediated transmission. Eur. J. Neurosci. 2008, 27:2783-2802.
3. Garbers D.L., Chrisman T.D., Wiegn P., Katafuchi T., Albanesi J.P., Bielinski V., Barylko B., Redfield M.M., Burnett J.C. Membrane guanylyl cyclase receptors: an update. Trends Endocrinol. Metab. 2006, 17:251-258.
4. Russwurm M., Koesling D. Isoforms of NO-sensitive guanylyl cyclase. Mol. Cell. Biochem. 2002, 230:159-164.
5. Denninger J.W., Marletta M.A. Guanylate cyclase and the NO/cGMP signaling pathway. Biochim. Biophys. Acta 1999, 1411:334-350.
6. Lincoln T.M., Cornwell T.L., Komalavilas P., Boerth N. Cyclic GMP-dependent protein kinase in nitric oxide signaling. Methods Enzymol. 1996, 269:149-166.
7. Conti M., Beavo J. Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling. Annu. Rev. Biochem. 2007, 76:481-511.
8. Kaupp U.B., Seifert R. Cyclic nucleotide-gated ion channels. Physiol. Rev. 2002, 82:769-824.
9. Russwurm M., Behrends S., Harteneck C., Koesling D. Functional properties of a naturally occurring isoform of soluble guanylyl cyclase. Biochem. J. 1998, 335:125-130.
10. Gibb B.J., Garthwaite J. Subunits of the nitric oxide receptor, soluble guanylyl cyclase, expressed in rat brain. Eur. J. Neurosci. 2001, 13:539-544.
11. Griffiths C., Wykes V., Bellamy T.C., Garthwaite J. A new and simple method for delivering clamped nitric oxide concentrations in the physiological range: application to activation of guanylyl cyclase-coupled nitric oxide receptors. Mol. Pharmacol. 2003, 64:1349-1356.
12. Traylor T.G., Sharma V.S. Why NO?. Biochemistry 1992, 31:2847-2849.
13. Roy B., Halvey E.J., Garthwaite J. An enzyme-linked receptor mechanism for nitric oxide-activated guanylyl cyclase. J. Biol. Chem. 2008, 283:18841-18851.
14. Bellamy T.C., Garthwaite J. Sub-second kinetics of the nitric oxide receptor, soluble guanylyl cyclase, in intact cerebellar cells. J. Biol. Chem. 2001, 276:4287-4292.
15. Liu Y., Ruoho A.E., Rao V.D., Hurley J.H. Catalytic mechanism of the adenylyl and guanylyl cyclases: modeling and mutational analysis. Proc. Natl. Acad. Sci. USA 1997, 94:13414-13419.
16. Senter P.D., Eckstein F., Mulsch A., Bohme E. The stereochemical course of the reaction catalyzed by soluble bovine lung guanylate cyclase. J. Biol. Chem. 1983, 258:6741-6745.
17. Moncada S. Nitric oxide: discovery and impact on clinical medicine. J. R. Soc. Med. 1999, 92:164-169.
18. Evgenov O.V., Pacher P., Schmidt P.M., Hasko G., Schmidt H.H., Stasch J.P. NO-independent stimulators and activators of soluble guanylate cyclase: discovery and therapeutic potential. Nat. Rev. Drug Discov. 2006, 5:755-768.
19. Bivalacqua T.J., Champion H.C., Hellstrom W.J., Kadowitz P.J. Pharmacotherapy for erectile dysfunction. Trends Pharmacol. Sci. 2000, 21:484-489.
20. Honda A., Adams S.R., Sawyer C.L., Lev-Ram V., Tsien R.Y., Dostmann W.R. Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator. Proc. Natl. Acad. Sci. USA 2001, 98:2437-2442.
21. Wunder F., Stasch J.P., Hutter J., Alonso-Alija C., Huser J., Lohrmann E. A cell-based cGMP assay useful for ultra-high-throughput screening and identification of modulators of the nitric oxide/cGMP pathway. Anal. Biochem. 2005, 339:104-112.
22. Schmidt K., Desch W., Klatt P., Kukovetz W.R., Mayer B. Release of nitric oxide from donors with known half-life: a mathematical model for calculating nitric oxide concentrations in aerobic solutions. Naunyn Schmiedebergs Arch. Pharmacol. 1997, 355:457-462.
23. Roy B., Mo E., Vernon J., Garthwaite J. Probing the presence of the ligand-binding haem in cellular nitric oxide receptors. Br. J. Pharmacol. 2008, 153:1495-1504.
24. Lee Y.C., Martin E., Murad F. Human recombinant soluble guanylyl cyclase: expression, purification, and regulation. Proc. Natl. Acad. Sci. USA 2000, 97:10763-10768.
25. Eccleston J.F., Moore K.J., Brownbridge G.G., Webb M.R., Lowe P.N. Fluorescence approaches to the study of the p21ras GTPase mechanism. Biochem. Soc. Trans. 1991, 19:432-437.
26. Garthwaite J. New insight into the functioning of nitric oxide-receptive guanylyl cyclase: physiological and pharmacological implications. Mol. Cell. Biochem. 2010, 334:221-232.
27. Hall C.N., Garthwaite J. What is the real physiological NO concentration in vivo?. Nitric Oxide 2009, 21:92-103.
28. Johnson J.D., Walters J.D., Mills J.S. A continuous fluorescence assay for cyclic nucleotide phosphodiesterase hydrolysis of cyclic GMP. Anal. Biochem. 1987, 162:291-295.
29. Hartell N.A., Furuya S., Jacoby S., Okada D. Intercellular action of nitric oxide increases cGMP in cerebellar Purkinje cells. NeuroReport 2001, 12:25-28.
30. Bellamy T.C., Garthwaite J. CAMP-specific phosphodiesterase contributes to cGMP degradation in cerebellar cells exposed to nitric oxide. Mol. Pharmacol. 2001, 59:54-61.
31. Vadakkadathmeethal K., Cunliffe J.M., Swift J., Kennedy R.T., Neubig R.R., Sunahara R.K. Fluorescence-based adenylyl cyclase assay adaptable to high throughput screening. Comb. Chem. High Throughput Screen. 2007, 10:289-298.