Analysis of testosterone and dihydrotestosterone in mouse tissues by liquid chromatography-electrospray ionization-tandem mass spectrometry

Analytical Biochemistry

Volumn 402, Issue 2, 2010, Pages 121-128

  Weng, Yan ^a   Xie, Fang ^a   Xu, Li ^a   Zagorevski, Dmitri ^b   Spink, David C ^a   Ding, Xinxin ^a  

^a STATE UNIVERSITY OF NEW YORK   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Cytochrome P450; Dihydrotestosterone; LC MS; Metabolism; Mice; Steroids; Testosterone

Indexed keywords

CHARCOAL; ELECTROSPRAY IONIZATION; HISTOLOGY; LIQUID CHROMATOGRAPHY; MAMMALS; MASS SPECTROMETRY; METABOLISM;

CYTOCHROME P450; DIHYDROTESTOSTERONE; LC-MS; MICE; STEROIDS; TESTOSTERONE;

TISSUE;

ANDROSTANOLONE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE FERRIHEMOPROTEIN REDUCTASE; TESTOSTERONE;

ACCURACY; ANDROGEN BLOOD LEVEL; ANIMAL TISSUE; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DERIVATIZATION; ELECTROSPRAY MASS SPECTROMETRY; FEMALE; FRAGMENTATION REACTION; HORMONE DETERMINATION; LIQUID CHROMATOGRAPHY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; TISSUE LEVEL; TRANSGENIC MOUSE; VALIDATION STUDY; WILD TYPE;

MURINAE; MUS; MUS MUSCULUS;

EID: 77953056849     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.03.034     Document Type: Article

References (40)

1. Papalia M.A., Davis S.R. What is the rationale for androgen therapy for women?. Treat. Endocrinol. 2003, 2:77-84.
2. Wilson J.D., Leihy M.W., Shaw G., Renfree M.B. Androgen physiology: unsolved problems at the millennium. Mol. Cell. Endocrinol. 2002, 198:1-5.
3. Basaria S., Dobs A.S. Hypogonadism and androgen replacement therapy in elderly men. Am. J. Med. 2001, 110:563-572.
4. Derman R.J. Effects of sex steroids on women's health: implications for practitioners. Am. J. Med. 1995, 98:137S-143S.
5. Bachmann G., Bancroft J., Braunstein G., Burger H., Davis S., Dennerstein L., Goldstein I., Guay A., Leiblum S., Lobo R., Notelovitz M., Rosen R., Sarrel P., Sherwin B., Simon J., Simpson E., Shifren J., Spark R., Traish A. Female androgen insufficiency: the Princeton consensus statement on definition, classification, and assessment. Fertil. Steril. 2002, 77:660-665.
6. Andriole G., Bruchovsky N., Chung L.W., Matsumoto A.M., Rittmaster R., Roehrborn C., Russell D., Tindall D. Dihydrotestosterone and the prostate: the scientific rationale for 5α-reductase inhibitors in the treatment of benign prostatic hyperplasia. J. Urol. 2004, 172:1399-1403.
7. Imperato-McGinley J., Zhu Y.S. Androgens and male physiology: the syndrome of 5α-reductase-2 deficiency. Mol. Cell. Endocrinol. 2002, 198:51-59.
8. Clark R.V., Hermann D.J., Cunningham G.R., Wilson T.H., Morrill B.B., Hobbs S. Marked suppression of dihydrotestosterone in men with benign prostatic hyperplasia by dutasteride, a dual 5α-reductase inhibitor. J. Clin. Endocrinol. Metab. 2004, 89:2179-2184.
9. Rittmaster R.S., Stoner E., Thompson D.L., Nance D., Lasseter K.C. Effect of MK-906, a specific 5α-reductase inhibitor, on serum androgens and androgen conjugates in normal men. J. Androl. 1989, 10:259-262.
10. Steidle C., Schwartz S., Jacoby K., Sebree T., Smith T., Bachand R. AA2500 testosterone gel normalizes androgen levels in aging males with improvements in body composition and sexual function. J. Clin. Endocrinol. Metab. 2003, 88:2673-2681.
11. Swerdloff R.S., Wang C., Cunningham G., Dobs A., Iranmanesh A., Matsumoto A.M., Snyder P.J., Weber T., Longstreth J., Berman N. Long-term pharmacokinetics of transdermal testosterone gel in hypogonadal men. J. Clin. Endocrinol. Metab. 2000, 85:4500-4510.
12. Funderburgh L.J., Zipf W.B., Sotos J.F. Direct measurement of testosterone in a pediatric center, with use of a radioimmunoassay kit and unextracted serum. Clin. Chem. 1983, 29:1796-1798.
13. Pazzagli M., Forti G., Cappellini A., Serio M. Radioimmunoassay of plasma dihydrotestosterone in normal and hypogonodal men. Clin. Endocrinol. (Oxf.) 1975, 4:513-520.
14. Pirke K.M., Doerr P. Plasma dihydrotestosterone in normal adult males and its relation to testosterone. Acta Endocrinol. (Copenh.) 1975, 79:357-365.
15. Wang C., Iranmanesh A., Berman N., McDonald V., Steiner B., Ziel F., Faulkner S.M., Dudley R.E., Veldhuis J.D., Swerdloff R.S. Comparative pharmacokinetics of three doses of percutaneous dihydrotestosterone gel in healthy elderly men: a clinical research center study. J. Clin. Endocrinol. Metab. 1998, 83:2749-2757.
16. Cawood M.L., Field H.P., Ford C.G., Gillingwater S., Kicman A., Cowan D., Barth J.H. Testosterone measurement by isotope dilution-liquid chromatography-tandem mass spectrometry: validation of a method for routine clinical practice. Clin. Chem. 2005, 51:1472-1479.
17. Taieb J., Mathian B., Millot F., Patricot M.C., Mathieu E., Queyrel N., Lacroix I., Somma-Delpero C., Boudou P. Testosterone measured by 10 immunoassays and by isotope dilution-gas chromatography-mass spectrometry in sera from 116 men, women, and children. Clin. Chem. 2003, 49:1381-1395.
18. Turpeinen U., Linko S., Itkonen O., Hamalainen E. Determination of testosterone in serum by liquid chromatography-tandem mass spectrometry. Scand. J. Clin. Lab. Invest. 2008, 68:50-57.
19. Wang C., Catlin D.H., Starcevic B., Leung A., DiStefano E., Lucas G., Hull L., Swerdloff R.S. Testosterone metabolic clearance and production rates determined by stable isotope dilution/tandem mass spectrometry in normal men: influence of ethnicity and age. J. Clin. Endocrinol. Metab. 2004, 89:2936-2941.
20. Kwan T.K., Trafford D.J., Makin H.L., Mallet A.I., Gower D.B. GC-MS studies of 16-androstenes and other C19 steroids in human semen. J. Steroid Biochem. Mol. Biol. 1992, 43:549-556.
21. Moneti G., Giovannini M.G., Guarna A., Forti G., Salerno R., Magini A., Serio M. Determination of testosterone and its tissue metabolites (DHT and 3α-diol) in human plasma and prostatic tissue by isotopic dilution mass spectrometry. J. Steroid Biochem. 1987, 27:53-59.
22. Abraham G.E., Manlimos F.S., Solis M., Wickman A.C. Combined radioimmunoassay of four steroids in one ml of plasma: II. Androgens. Clin. Biochem. 1975, 8:374-378.
23. Werawatgoompa S., Dusitsin N., Sooksamiti P., Leepipatpaiboon S., Virutamasen P., Boonsiri B. A rapid method for the determination of 5α-dihydrotestosterone in Thai males receiving medroxyprogesterone acetate. Contraception 1982, 25:523-533.
24. Higashi T., Ninomiya Y., Shimada K. Studies on neurosteroids: XX. Liquid chromatography-tandem mass spectrometric method for simultaneous determination of testosterone and 5α-dihydrotestosterone in rat brain and serum. J. Chromatogr. Sci. 2008, 46:653-658.
25. Higashi T., Yamauchi A., Shimada K. 2-Hydrazino-1-methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization-mass spectrometry. J. Chromatogr. B 2005, 825:214-222.
26. Higashi T., Yamauchi A., Shimada K., Koh E., Mizokami A., Namiki M. Determination of prostatic androgens in 10mg of tissue using liquid chromatography-tandem mass spectrometry with charged derivatization. Anal. Bioanal. Chem. 2005, 382:1035-1043.
27. Kalhorn T.F., Page S.T., Howald W.N., Mostaghel E.A., Nelson P.S. Analysis of testosterone and dihydrotestosterone from biological fluids as the oxime derivatives using high-performance liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2007, 21:3200-3206.
28. Kashiwagi B., Shibata Y., Ono Y., Suzuki K., Honma S., Yamanaka H. Effect of androgen concentration on seminal vesicle blood flow in rats: establishment of new highly sensitive simultaneous androgen measurement method. Urology 2005, 66:218-223.
29. Kashiwagi B., Shibata Y., Ono Y., Suzuki R., Honma S., Suzuki K. Changes in testosterone and dihydrotestosterone levels in male rat accessory sex organs, serum, and seminal fluid after castration: establishment of a new highly sensitive simultaneous androgen measurement method. J. Androl. 2005, 26:586-591.
30. Licea-Perez H., Wang S., Szapacs M.E., Yang E. Development of a highly sensitive and selective UPLC/MS/MS method for the simultaneous determination of testosterone and 5α-dihydrotestosterone in human serum to support testosterone replacement therapy for hypogonadism. Steroids 2008, 73:601-610.
31. Wu L., Gu J., Cui H., Zhang Q.Y., Behr M., Fang C., Weng Y., Kluetzman K., Swiatek P.J., Yang W., Kaminsky L., Ding X. Transgenic mice with a hypomorphic NADPH-cytochrome P450 reductase gene: effects on development, reproduction, and microsomal cytochrome P450. J. Pharmacol. Exp. Ther. 2005, 312:35-43.
32. Williams T.M., Kind A.J., Houghton E., Hill D.W. Electrospray collision-induced dissociation of testosterone and testosterone hydroxy analogs. J. Mass Spectrom. 1999, 34:206-216.
33. Shiraishi S., Lee P.W.N., Leung A., Goh V.H.H., Swerdloff R.S., Wang C. Simultaneous measurement of serum testosterone and dihydrotestosterone by liquid chromatography-tandem mass spectrometry. Clin. Chem. 2008, 54:1855-1863.
34. Gu J., Dudley C., Su T., Spink D.C., Zhang Q.Y., Moss R.L., Ding X. Cytochrome P450 and steroid hydroxylase activity in mouse olfactory and vomeronasal mucosa. Biochem. Biophys. Res. Commun. 1999, 266:262-267.
35. Maines M.D., Mayer R.D. Inhibition of testicular cytochrome P-450-dependent steroid biosynthesis by cis-platinum: reversal by human chorionic gonadotropin. J. Biol. Chem. 1985, 260:6063-6068.
36. Park T.J., Song K.Y., Sohn S.H., Lim I.K. Marked inhibition of testosterone biosynthesis by the hepatotoxin nodularin due to apoptosis of Leydig cells. Mol. Carcinog. 2002, 34:151-163.
37. Jeyaraj D.A., Grossman G., Petrusz P. Altered bioavailability of testosterone in androgen-binding protein-transgenic mice. Steroids 2005, 70:704-714.
38. Griffin J.E., Wilson J.D. Testicular disorders. Williams Textbook of Endocrinology 2007, 652-672. Elsevier, Philadelphia. 11th ed. H.M. Kronenberg, S. Melmed, P.R. Larsen, K.S. Polonsky (Eds.).
39. Waxman D.J. Interactions of hepatic cytochromes P-450 with steroid hormones: regioselectivity and stereospecificity of steroid metabolism and hormonal regulation of rat P-450 enzyme expression. Biochem. Pharmacol. 1988, 37:71-84.
40. Pardridge W.M. Transport of nutrients and hormones through the blood-brain barrier. Diabetologia 1981, 20:246-254.