Directed evolution of a thermophilic β-glucosidase for cellulosic bioethanol production

Applied Biochemistry and Biotechnology

Volumn 161, Issue 1-8, 2010, Pages 301-312

  Hardiman, Elizabeth ^a   Gibbs, Moreland ^a,b   Reeves, Rosalind ^a,b   Bergquist, Peter ^a,b,c  

^a MACQUARIE UNIVERSITY   (Australia)

^b Applimex Systems Pty Ltd   (Australia)

^c UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

glucosidase; Directed evolution; Fluorescence activated cell sorting; In vitro compartmentalisation; Random drift mutagenesis

Indexed keywords

DIRECTED EVOLUTION; FLUORESCENCE ACTIVATED CELL SORTING; GLUCOSIDASE; IN VITRO COMPARTMENTALISATION; RANDOM DRIFT;

AMINO ACIDS; BACTERIA; BIOETHANOL; ENZYMES; ETHANOL; FLUORESCENCE; INDUSTRIAL APPLICATIONS; LIBRARIES; SUBSTRATES; SUGARS;

CELLS;

ALCOHOL; BETA GLUCOSIDASE; CELLOBIOSE; CELLULOSE; GALACTOSE; GLUCAN SYNTHASE; LACTOSE; PYRANOSIDE; BACTERIAL PROTEIN; BIOFUEL;

ALCOHOL PRODUCTION; AMINO ACID SUBSTITUTION; ARTICLE; CELL ENCAPSULATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE; FLUORESCENCE ACTIVATED CELL SORTING; GENE SEQUENCE; GENOTYPE; HIGH THROUGHPUT SCREENING; HYDROLYSIS; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; MUTAGENESIS; NONHUMAN; PHENOTYPE; THERMOPHILIC BACTERIUM; TURNOVER TIME; WILD TYPE; AMINO ACID SEQUENCE; DIRECTED MOLECULAR EVOLUTION; EVALUATION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; POINT MUTATION; PROTEIN ENGINEERING;

BACTERIA (MICROORGANISMS); CALDICELLULOSIRUPTOR SACCHAROLYTICUS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BETA-GLUCOSIDASE; BIOFUELS; DIRECTED MOLECULAR EVOLUTION; ETHANOL; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POINT MUTATION; PROTEIN ENGINEERING;

EID: 77953016106     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-009-8794-6     Document Type: Article

References (24)

1. Shewale, J. G. (1982). International Journal of Biochemistry, 14, 435-443.
2. Turner, P., Mamo, G., & Karlsson, E. (2007). Microbial Cell Factories, 6, 9.
3. Nakkharat, P., & Haltrich, D. (2006). Journal of Biotechnology, 123, 304-313.
4. Sun, Y., & Cheng, J. (2002). Bioresources Technology, 83, 1-11.
5. Saha, B. C., & Bothast, R. J. (1996). Applied and Environmental Microbiology, 62, 3165-3170.
6. Bommarius, A. S., Katona, A., Cheben, S. E., Patel, A. S., Ragauskas, A. J., Knudson, K., et al. (2008). Metabolic Engineering, 10, 370-381.
7. Novalin, S., Neuhaus, W., & Kulbe, K. D. (2005). Journal of Biotechnology, 119, 212-218.
8. Bruins, M. E., Janssen, A. E. M., & Boom, R. M. (2000). Applied Biochemistry and Biotechnology, 90, 155-186.
9. Bergquist, P. L., Reeves, R. A., & Gibbs, M. D. (2005). Biomolecular Engineering, 22, 63-72.
10. Gupta, R. D., & Tawfik, D. S. (2008). Nature Methods, 5, 939-942.
11. Bershtein, S., Goldin, K., & Tawfik, D. S. (2008). Journal of Molecular Biology, 379, 1029-1044.
12. Bershtein, S., & Tawfik, D. S. (151). Biocatalysis and biotransformation/Bioinorganic chemistry, 12, 158.
13. Aharoni, A., Griffiths, A. D., & Tawfik, D. S. (2005). Current Opinion in Chemical Biology, 9, 210-216.
14. Tawfik, D. S., & Griffiths, A. D. (1998). Nature Biotechnology, 16, 652-656.
15. Miller, O. J., Bernath, K., Agresti, J. J., Amitai, G., Kelly, B. T., Mastrobattista, E., et al. (2006). Nature Methods, 3, 561-570.
16. Bernath, K., Hai, M., Mastrobattista, E., Griffiths, A., Magdassi, S., & Tawfik, D. S. (2003). Analytical Biochemistry, 325, 151-157.
17. Aharoni, A., Amitai, G., Bernath, K., Magdassi, S., & Tawfik, D. S. (2005). Chemistry and Biology, 12, 1281-1289.
18. Love, D. R., Fisher, R., & Bergquist, P. L. (1988). Molecular Genetics and Genomics, 213, 84-92.
19. Cadwell, R. C., & Joyce, G. F. (1992). PCR Methods and Applications, 2, 28-33.
20. Rothe, A., Surjadi, R. N., & Power, B. E. (2006). Trends in Biotechnology, 24, 587-592.
21. Beveridge, T. J. (1999). Journal of Bacteriology, 181, 4725-4733.
22. Roodveldt, C., Aharoni, A., & Tawfik, D. S. (2005). Current Opinion in Structural Biology, 15, 50-56.
23. Gloster, T. M., Macdonald, J. M., Tarling, C. A., Stick, R. V., Withers, S. G., & Davies, G. J. (2004). The Journal of Biological Chemistry, 279, 49236-49242.
24. Bergquist, P., Hardiman, E., Ferrari, B., & Winsley, T. (2009). Extremophiles, 3, 389-401.