Cyanobacterial extracts and microcystin-LR are inactive in the micronucleus assay in vivo and in vitro

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 699, Issue 1-2, 2010, Pages 5-10

  Abramsson Zetterberg, Lilianne ^a   Sundh, Ulla Beckman ^a   Mattsson, Roland ^b  

^a NATIONAL FOOD ADMINISTRATION   (Sweden)

^b NATIONAL VETERINARY INSTITUTE   (Sweden)

Author keywords

Cyanobacteria extract; Flow cytometer; Microcystin LR; Micronuclei

Indexed keywords

CELL EXTRACT; CYANOBACTERIUM EXTRACT; MICROCYSTIN LR; UNCLASSIFIED DRUG;

ADULT; ANIMAL EXPERIMENT; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYANOBACTERIUM; ERYTHROCYTE; FEMALE; FLOW CYTOMETRY; GENOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; LAKE; LETHALITY; LYMPHOCYTE; MALE; MICRONUCLEUS TEST; MOUSE; NONHUMAN; NORMAL HUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SWEDEN;

ANIMALS; BACTERIAL TOXINS; CYANOBACTERIA; FLOW CYTOMETRY; HUMANS; MARINE TOXINS; MICE; MICROCYSTINS; MICRONUCLEUS TESTS; SWEDEN; WATER MICROBIOLOGY;

ANIMALIA; BOS; CANIS FAMILIARIS; CYANOBACTERIA;

EID: 77952582025     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2010.04.001     Document Type: Article

References (49)

1. WHO, Cyanobacterial toxins: microcystin-LR in drinking-water. Background document for preparation of WHO Guidelines for drinking-water quality, Geneva, World Health Organization (WHO/SDE/WSH/03.04/57), 2003.
2. Falconer I.R. Tumor promotion and liver injury caused by oral consumption of cyanobacteria. Environ. Toxicol. Water Qual. 6 (1991) 177-184
3. Falconer I.R., and Buckley T.H. Tumour promotion by Microcystis sp., a blue-green alga occurring in water supplies. Med. J. Aust. 150 (1989) 351
4. Nishiwaki-Matsushima R., Ohta T., Nishiwaki S., Suganuma M., Kohyama K., Ishikawa T., Carmichael W.W., and Fujiki H. Liver tumor promotion by the cyanobacterial cyclic peptide toxin microcystin-LR. J. Cancer Res. Clin. Oncol. 118 (1992) 420-424
5. Ohta T., Sueoka E., Iida N., Komori A., Suganuma M., et al. Nodularin, a potent inhibitor of protein phosphatases 1 and 2A, is a new environmental carcinogen in male F344 rat liver. Cancer Res. 54 (1994) 6402-6406
6. Falconer I.R., and Humpage A.R. Tumour promotion by cyanobacterial toxins. Phycologia 35 (1996) 74-79
7. Ueno Y., Makita Y., Nagata S., Tsutsumi T., et al. No chronic oral toxicity of a low dose of microcystin-LR, a cyanobacterial hapatotoxin, in female BALB/c mice. Environ. Toxicol. 14 (1999) 45-55
8. Ito E., Kondo F., Terao K., and Harada K.I. Neoplastic nodular formation in mouse liver induced by repeated intraperitoneal injections of microcystin-LR. Toxicon 35 (1997) 1453-1457
9. cen, draft ISO/DIS 20179 Water quality - Determination of microcystines - Method using solid phase extraction (SPE) and high performance liquid chromatography (HPLC) with ultraviolet (UV) detection, 2004.
10. Abramsson-Zetterberg L., Zetterberg G., and Grawé J. The time course of micronucleated polychromatic erythrocytes in mouse bone marrow and peripheral blood. Mutat. Res. 350 (1996) 349-358
11. Grawé J., Zetterberg G., and Amnéus H. Flow-cytometric enumeration of micronucleated polychromatic erythrocytes in mouse peripheral blood. Cytometry 13 (1992) 750-758
12. 137Cs at very low dose rates. Int. J. Radiat. Biol. 67 (1995) 29-36
13. Hayashi H., Norppa H., Sofuni Jr. T., and Ishidate M. Flow cytometric micronucleus test with mouse peripheral erythrocytes. Mutagenesis 7 (1992) 257-264
14. Fenech M., and Morley A.A. Measurement of micronuclei in lymphocytes. Mutat. Res. 147 (1985) 29-36
15. Migliore L., Scarpato R., and Falco P. The use of fluorescence in situ hybridization with a beta-satellite DNA probe for the detection of acrocentric chromosomes in vanadium-induced micronuclei. Cytogenet. Cell Genet. 69 (1995) 215-219
16. Fenech M. The in vitro micronucleus technique. Mutat. Res. 455 (2000) 81-95
17. Sivonen K., and Jones G. Cyanobacterial toxins. In: Chorus I., and Bartram E. (Eds). Toxic Cyanobacteria in Water (1999), E & FN Spon, London and New York 41-111
18. 3H-dihydromicrocystin-LR, a cyclic peptide toxin. Biochim. Biophys. Acta 1025 (1990) 60-66
19. Runnegar M.T.C., Gerdes R.G., and Falconer I.R. The uptake of the cyanobacterial hepatotoxin microcystin by isolated rat hepatocytes. Toxicon 29 (1991) 43-51
20. Runnegar M., Bergdt N., and Kaplowitz N. Microcystin uptake and inhibition of protein phosphatases: Effects of chemoprotectants and self-inhibition in relation to known hepatic transporters. Toxicol. Appl. Pharmacol. 134 (1995) 264-272
21. Lu H., Choudhuri S., Ogura K., Csanaky I.L., Lei X., Cheng X., Song P., and Klaassen C.D. Characterization of organic anion transporting polypeptide 1b2-null mice: essential role in hepatic uptake/toxicity of phalloidin and microcystin-LR. Toxicol. Sci. 103 (2008) 35-45
22. Fischer W.J., Altheimer S., Cattori V., Meier P.J., Dietrich D.R., and Hagenbuch B. Organic anion transporting polypeptides expressed in liver and brain mediate uptake of microcystin. Toxicol. Appl. Pharmacol. 203 (2005) 257-263
23. Falconer I.R., Buckley T., and Runnegar M.T. Biological half-life, organ distribution and excretion of 125-I-labelled toxic peptide from the blue-green alga Microcystis aeruginosa. Aust. J. Biol. Sci. 39 (1986) 17-21
24. Robinson N.A., Miura G.A., Matson C.F., Dinterman R.E., and Pace J.G. Characterization of chemically tritiated microcystin-LR and its distribution in mice. Toxicon 27 (1989) 1035-1042
25. Robinson N.A., Pace J.G., Matson C.F., Miura G.A., and Lawrence W.B. Tissue distribution, excretion and hepatic biotransformation of microcystin-LR in mice. J. Pharmacol. Exp. Ther. 256 (1991) 176-182
26. Brooks W.P., and Codd G.A. Distribution of Microcystis aeruginosa peptide toxin and interactions with hepatic microsomes in mice. Pharmacol. Toxicol. 60 (1987) 187-191
27. Wiegand C., Pflugmacher S., Oberemm A., Meems N., Beattie K.A., Steinberg C.E.W., and Codd G.A. Uptake and effects of microcystin-LR on detoxication enzymes of early life stages of the zebra fish (Danio rerio). Environ. Toxicol. 14 (1999) 89-95
28. Kurki-Helasmo K., and Meriluoto J. Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings. Toxicon 36 (1998) 1921-1926
29. Pflugmacher S., Wiegand C., Oberemm A., Beattie K.A., Krause E., Codd G.A., and Steinberg C.E. Identification of an enzymatically formed glutathione conjugate of the cyanobacterial hepatotoxin microcystin-LR: the first step of detoxication. Biochim. Biophys. Acta 1425 (1998) 527-533
30. Lankoff A., Carmichael W.W., Grasman K.A., and Yuan M. The uptake kinetics and immunotoxic effects of microcystin-LR in human and chicken peripheral blood lymphocytes in vitro. Toxicology 204 (2004) 23-40
31. Ding W.X., Shen H.M., Zhu H.G., Lee B.L., and Ong C.N. Genotoxicity of microcystic cyanobacteria extract of a water source in China. Mutat. Res. 442 (1999) 69-77
32. Abramsson-Zetterberg L. The dose-response relationship at very low doses of acrylamide is linear in the flow cytometer-based mouse micronucleus assay. Mutat. Res. 535 (2003) 215-222
33. Grawé J., Abramsson-Zetterberg L., and Zetterberg G. Low dose effects of chemicals as assessed by the flow cytometric in vivo micronucleus assay. Mutat. Res. 405 (1998) 199-208
34. Gaudin J., Huet S., Jarry G., and Fessard V. In vivo DNA damage induced by the cyanotoxin microcystin-LR: comparison of intra-peritoneal and oral administrations by use of the comet assay. Mutat. Res. 652 (2008) 65-71
35. Mankiewicz J., Walter Z., Tarczynska M., Palyvoda O., Wojtysiak-Staniaszczyk M., and Zalewski M. Genotoxicity of cyanobacterial extracts containing microcystins from Polish water reservoirs as determined by SOS chromotest and comet assay. Environ. Toxicol. 17 (2002) 341-350
36. Zhan L., Sakamoto H., Sakuraba M., Wu D.S., Zhang L.S., Suzuki T., Hayashi M., and Honma M. Genotoxicity of microcystin-LR in human lymphoblastoid TK6 cells. Mutat. Res. 557 (2004) 1-6
37. Humpage A.R., Fenech M., Thomas P., and Falconer I.R. Micronucleus induction and chromosome loss in transformed human white cells indicate clastogenic and aneugenic action of the cyanobacterial toxin, cylindrospermopsin. Mutat. Res. 472 (2000) 155-161
38. Lankoff A., Banasik A., Obe G., Deperas M., Kuzminski K., Tarczynska M., Jurczak T., and Wojcik A. Effect of microcystin-LR and cyanobacterial extract from Polish reservoir of drinking water on cell cycle progression, mitotic spindle, and apoptosis in CHO-K1 cells. Toxicol. Appl. Pharmacol. 189 (2003) 204-213
39. Collins M., Gowans C., Garro F., Estervig D., and Swanson T. Temporal association between an algal bloom and mutagenicity in a water reservoir. In: Carmichael W.W. (Ed). The Water Environment, Algal Toxins and Health (1981), Plenum Press, New York and London 271-284
40. Repavich W.M., Sonzogni W.C., Standridge J.H., Wedepohl R.E., and Meisner L.F. Cyanobacteria (blue green algae) in Wisconsin waters: acute and chronic toxicity. Water Res. 24 (1990) 225-231
41. Wu J.Y., Xu O.J., Gao G., and Shen J.H. Evaluating genotoxicity associated with microcystin-LR and its risk to source water safety in Meiliang Bay, Taihu Lake. Environ. Toxicol. 21 (2006) 250-255
42. Kondo F., Ikai Y., Oka H., Okumura M., Ishikawa N., Harada K., Matsuura K., Murata H., and Suzuki M. Formation, characterization, and toxicity of the glutathione and cysteine conjugates of toxic heptapeptide microcystins. Chem. Res. Toxicol. 5 (1992) 591-596
43. Metcalf J.S., Beattie K.A., Pflugmacher S., and Codd G.A. Immuno-crossreactivity and toxicity assessment of conjugation products of the cyanobacterial toxin, microcystin-LR. FEMS Microbiol. Lett. 189 (2000) 155-158
44. Rao P.V., and Bhattacharya R. The cyanobacterial toxin microcystin-LR induced DNA damage in mouse liver in vivo. Toxicology 114 (1996) 29-36
45. Zegura B., Sedmak B., and Filipic M. Microcystin-LR induces oxidative DNA damage in human hepatoma cell line HepG2. Toxicon 41 (2003) 41-48
46. Maatouk I., Bouaïcha N., Plessis M.J., and Périn F. Detection by 32P-postlabelling of 8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA as biomarker of microcystin-LR- and nodularin-induced DNA damage in vitro in primary cultured rat hepatocytes and in vivo in rat liver. Mutat. Res. 14 (2004) 9-20
47. Nong Q., Komatsu M., Izumo K., Indo H.P., Xu B., Aoyama K., Majima H.J., Horiuchi M., Morimoto K., and Takeuchi T. Involvement of reactive oxygen species in Microcystin-LR-induced cytogenotoxicity. Free Radic. Res. 41 (2007) 1326-1337
48. Gaudin J., Le Hegarat L., Nesslany F., Marzin D., and Fessard V. In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration. Environ. Toxicol. 24 (2009) 200-209
49. Steeg P.S., Palmieri D., Ouatas T., and Salerno M. Histidine kinases and histidine phosphorylated proteins in mammalian cell biology, signal transduction and cancer. Cancer Lett. 190 (2003) 1-12