The SbcCD complex of Deinococcus radiodurans contributes to radioresistance and DNA strand break repair in vivo and exhibits Mre11-Rad50 type activity in vitro

DNA Repair

Volumn 9, Issue 5, 2010, Pages 488-494

  Kamble, Vidya A ^a   Misra, Hari S ^a  

^a BHABHA ATOMIC RESEARCH CENTRE   (India)

Author keywords

Deinococcus; DSB repair; Homologous recombination; Mre11 Rad50 activity; Radioresistance; SbcCD complex

Indexed keywords

BACTERIAL PROTEIN; ENDONUCLEASE; EXONUCLEASE; HYDROGEN PEROXIDE; MITOMYCIN C; MRE11 PROTEIN; PROTEIN SBCCD; RAD50 PROTEIN; RECA PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; CELL SURVIVAL; COMPLEX FORMATION; CONTROLLED STUDY; DEINOCOCCUS RADIODURANS; DNA DEGRADATION; DNA REPAIR; DNA STRAND BREAKAGE; ENZYME ACTIVITY; GAMMA RADIATION; GENE MUTATION; IN VIVO STUDY; MUTANT; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE; RADIOSENSITIVITY; WILD TYPE;

BACTERIAL PROTEINS; DEINOCOCCUS; DEOXYRIBONUCLEASES; DNA BREAKS, DOUBLE-STRANDED; DNA REPAIR; DNA REPAIR ENZYMES; DNA, BACTERIAL; DNA-BINDING PROTEINS; ENDODEOXYRIBONUCLEASES; GAMMA RAYS; RADIATION TOLERANCE; SEQUENCE DELETION; SOLUTIONS;

BACTERIA (MICROORGANISMS); DEINOCOCCUS; DEINOCOCCUS RADIODURANS; ESCHERICHIA COLI;

EID: 77952106953     PISSN: 15687864     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dnarep.2010.01.012     Document Type: Article

References (47)

1. Kowalczykowski S.C., Dixon D.A., Eggleston A.K., Louder S.D., and Rehrauer W.M. Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58 (1994) 401-465
2. Kuzminov A. Recombination repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63 (1999) 751-813
3. Clark A.J., and Sandler S.J. Homologous genetic recombination: the pieces begin to fall into place. Crit. Rev. Microbiol. 20 (1994) 125-142
4. Lloyd R.G., and Buckman C. Identification and genetic analysis of sbcC mutations in commonly used recBC, sbcB strains of Escherichia coli K12. J. Bacteriol. 164 (1988) 836-844
5. Connelly J.C., and Leach D.R. The sbcC and sbcD genes of Escherichia coli encode a nuclease involved in palindrome inviability and genetic recombination. Genes Cells 1 (1996) 285-291
6. Leach D.R.F. Long DNA palindromes, cruciform structures, genetic instability and secondary structure repair. Bioessay 16 (1994) 893-900
7. Chalker A.F., Leach D.R., and Llyod R.G. Escherichia coli sbcC mutant permit stable propagation of DNA replicons containing a long palindrome. Gene 71 (1988) 201-205
8. Gibson F.P., Leach D.R.F., and Llyod R.G. Identification of sbcD mutations as cosuppressors of recBC that allow propagation of DNA palindromes in Escherichia coli K-12. J. Bacteriol. 174 (1992) 1222-1228
9. Kieser T., and Melton R. Plasmid pIJ699, a multi-copy positive selection vector for Streptomyces. Gene 65 (1988) 83-91
10. Peeters B.P.H., De Boer J.H., Bron S., and Venema G. Structural plasmid instability in Bacillus subtilis: effect of direct and inverted repeats. Mol. Gen. Genet. 212 (1988) 450-458
11. Lobachev K.S., Shor B.M., Tran H.T., Taylor W., Keen J.D., Resnick M.A., and Gordenin D.A. Factors affecting inverted repeat stimulation of recombination and deletion in Saccharomyces cerevisiae. Genetics 148 (1998) 1507-1524
12. Kramer P.R., Stringer J.R., and Sinden R.R. Stability of an inverted repeat in a human fibrosarcoma cell. Nucleic Acid Res. 24 (1996) 4234-4241
13. Akgun E., Zahn J., Baumes S., Brown G., Liang F., Romaneinko P.J., Lewis S., and Jasin M. Palindrome resolution and recombination in the mammalian germ line. Mol. Cell Biol. 17 (1997) 5559-5570
14. Connelly J.C., deLeau E.S., and Leach D.R.F. DNA cleavage and degradation by the SbcCD protein complex from Escherichia coli. Nucleic Acid Res. 27 (1999) 1039-1046
15. Connelly J.C., deLeau E.S., and Leach D.R.F. Nucleolytic processing of a protein-bound DNA end by the E. coli SbcCD (MR) complex. DNA Repair 2 (2003) 795-807
16. Battista J.R. Against all odds, the survival strategies of Deinococcus radiodurans. Ann. Rev. Microbiol. 51 (1997) 203-224
17. Minton K.W. DNA repair in the extremely radioresistant bacterium Deinococcus radiodurans. Mol. Microbiol. 13 (1994) 9-15
18. Caimi P., and Eisenstark A. Sensitivity of Deinococcus radiodurans to near-ultraviolet radiation. Mutat. Res. 162 (1986) 145-151
19. Slade D., Lindner A.B., Paul G., and Radman M. Recombination and replication in DNA repair of heavily irradiated Deinococcus radiodurans. Cell 136 (2009) 1044-1055
20. Rocha E.P., Cornet E., and Michel B. Comparative and evolutionary analysis of the bacterial homologous recombination systems. PloS Genet. 1 (2005) e15
21. Bentchikou E., Servant P., Coste G., and Sommer S. Additive effects of SbcCD and PolX deficiencies in the in vivo repair of DNA double-strand breaks in Deinococcus radiodurans. J. Bacteriol. 189 (2007) 4784-4790
22. Khairnar N.P., Kamble V.A., and Misra H.S. RecBC enzyme overproduction affects UV and gamma radiation survival of Deinococcus radiodurans. DNA Repair 7 (2008) 40-47
23. Misra H.S., Khairnar N.P., Kota S., Srivastava S., Joshi V.P., and Apte S.K. An exonuclease I sensitive DNA repair pathway in Deinococcus radiodurans: a major determinant of radiation resistance. Mol. Microbiol. 59 (2006) 1308-1316
24. Schafer M., Schmitz C., Facius R., Horneck G., Milow B., Funken K.-K., and Ortner J. Systematic study of parameters influencing the action of Rose Bengal with visible light on bacterial cells: comparison between biological effect and singlet-oxygen production. Photochem. Photobiol. 71 (2000) 514-523
25. Sambrook J., and Russell D.W. Molecular Cloning: A Laboratory Manual. third ed. (2002), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
26. Battista J.R., Park M.J., and McLemore A.E. Inactivation of two homologous proteins presumed to be involved in the desiccation tolerance of plants sensitizes Deinococcus radiodurans R1 to desiccation. Cryobiology 43 (2001) 133-139
27. Harris D.R., Tanaka M., Saveliev S.V., Jolivet E., Earl A.M., Cox M.M., and Battista J.R. Preserving genome integrity: the DdrA protein of Deinococcus radiodurans R1. PloS Biol. 2 (2004) e304
28. Misra H.S., Pandey P.K., and Pandey V.N. An enzymatically active chimeric HIV-1 reverse transcriptase (RT) with the RNase-H domain of murine leukemia virus RT exists as a monomer. J. Biol. Chem. 273 (1998) 9785-9789
29. Evans D.M., and Moseley B.E.B. Roles of the uvsC, uvsD, uvsE and mtcA genes in the two-pyrimidine dimer excision repair pathway of Deinococcus radiodurans. J. Bacteriol. 156 (1983) 576-583
30. Rajpurohit Y.S., Gopalakrishnan R., and Misra H.S. Involvement of a protein kinase activity inducer in DNA double strand break repair and radioresistance of Deinococcus radiodurans. J. Bacteriol. 190 (2008) 3948-3954
31. Cromie G.A., and Leach D.F.R. Recombination repair of chromosomal DNA double strand breaks generated by a restriction endonuclease. Mol. Microbiol. 41 (2001) 873-883
32. Cromie G.A., Connelly J.C., and Leach D.F.R. Recombination at double strand breaks and DNA ends: conserved mechanism from phage to humans. Mol. Cell 8 (2001) 1163-1174
33. Paques F., and Haber E. Multiple pathways of recombination induced by double strand breaks in Saccharomyces cerevisae. Microbiol. Mol. Biol. Rev. 63 (1999) 349-404
34. Zahradka K., Slade D., Bailone A., Sommer S., Averbeck D., Petranovic M., Lindner A.B., and Radman M. Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature 443 (2006) 569-573
35. White O., Eisen J.A., Heildelberg J.F., Hickey E.K., Peterson J.D., and Dodson R.J. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286 (1999) 1571-1577
36. Makarova S., Aravind L., Wolf Y.I., Tatusov R.L., Minton K.W., Koonin E.V., and Daly M.J. Genome of extremely radiation resistant bacterium, Deinococcus radiodurans viewed from the perspectives of comparative genomics. Microbiol. Mol. Biol. Rev. 65 (2001) 45-79
37. Liu Y., Zhou J., Omelchenko M.V., Beliaev A.S., Venkateswaran A., and Stair J. Transcriptome dynamics of Deinococcus radiodurans recovering from ionizing radiation. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 4191-4196
38. Connelly J.C., de Leau E.S., Okely E.A., and Leach D.R.F. Overexpression, purification and characterization of the SbcCD protein from Escherichia coli. J. Biol. Chem. 272 (1997) 19819-19826
39. Daly M.J., Gaidamakova E.K., Matrosova V.Y., Vasilenko A., Zhai M., and Venkateswaran A. Accumulation of Mn(II) in Deinococcus radiodurans facilitates gamma-radiation resistance. Science 306 (2004) 1025-1028
40. Paull T.T., and Gellert M. The 3′ to 5′ exonuclease activity of Mre11 facilitates repair of DNA double strand breaks. Mol. Cell 1 (1998) 969-979
41. Trujillo K.M., and Sung P. DNA structure specific nuclease activities in the Saccharomyces cerevisae Rad50Mre11 complex. J. Biol. Chem. 276 (2001) 35458-35464
42. Daly M.J., and Minton K.W. An alternative pathway of recombination of chromosomal fragments precedes recA-dependent recombination in the radioresistant bacterium Deinococcus radiodurans. J. Bacteriol. 178 (1996) 4461-4471
43. Xu G., Wang L., Chen H., Lu H., Ying N., Tian B., and Hua Y. RecO is essential for DNA damage repair in Deinococcus radiodurans. J. Bacteriol. 190 (2008) 2624-2628
44. Connelly J.C., and Leach D.R. Tethering on the brink: the evolutionarily conserved Mre11-Rad50 complex. Trends Biochem. Sci. 27 (2002) 410-418
45. de Jagar M., van Noort J., van Gent D.C., Dekker C., Kanaar R., and Wyman C. Human Rad50-Mre11 is a flexible complex that can tether DNA ends. Mol. Cell 8 (2001) 1129-1135
46. Hofpner K.P., Putnam C.D., and Trainer J.A. DNA double strand break repair from head to tail. Curr. Opin. Struct. Biol. 12 (2002) 115-122
47. Mascarenhas J., Sanchez H., Tadesse S., Kidane D., Krishnamurthy M., Alonso J.C., and Graumann P.L. Bacillus subtilis SbcC protein plays an important role in DNA inter-strand cross-link repair. BMC Mol. Biol. 7 (2006) 20