메뉴 건너뛰기




Volumn 70, Issue 3, 2010, Pages 233-241

Enhancing the prebiotic relevance of a set of covalently self-assembling, autorecombining RNAs through in vitro selection

Author keywords

Autocatalysis; Azoarcus; Group I intron; In vitro selection; Molecular evolution; Recombination; Self replication

Indexed keywords

BACTERIAL RNA; PREBIOTIC AGENT; RECOMBINASE; RIBOZYME;

EID: 77952097812     PISSN: 00222844     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00239-010-9325-3     Document Type: Article
Times cited : (9)

References (42)
  • 2
    • 0037168445 scopus 로고    scopus 로고
    • Ribozyme-catalyzed excision of targeted sequences from within RNAs
    • DOI 10.1021/bi0267386
    • MA Bell AK Johnson SM Testa 2002 Ribozyme-catalyzed excision of targeted sequences from within RNAs Biochemistry 41 15327 15333 10.1021/bi0267386 1:CAS:528:DC%2BD38XovFygt7c%3D 12484771 (Pubitemid 36008142)
    • (2002) Biochemistry , vol.41 , Issue.51 , pp. 15327-15333
    • Bell, M.A.1    Johnson, A.K.2    Testa, S.M.3
  • 3
    • 57749185463 scopus 로고    scopus 로고
    • Parallel adaptations to high temperatures in the Archaean eon
    • 10.1038/nature07393 1:CAS:528:DC%2BD1cXhsFWmsb7N 19037246
    • B Boussau S Blanquart A Necsulea N Lartillot M Gouy 2008 Parallel adaptations to high temperatures in the Archaean eon Nature 456 942 945 10.1038/nature07393 1:CAS:528:DC%2BD1cXhsFWmsb7N 19037246
    • (2008) Nature , vol.456 , pp. 942-945
    • Boussau, B.1    Blanquart, S.2    Necsulea, A.3    Lartillot, N.4    Gouy, M.5
  • 4
    • 0031686486 scopus 로고    scopus 로고
    • Recombination, RNA evolution, and bifunctional RNA molecules isolated through chimeric SELEX
    • DOI 10.1017/S1355838298980542
    • DH Burke JH Willis 1998 Recombination, RNA evolution, and bifunctional RNA molecules isolated through chimeric SELEX RNA 4 1165 1175 10.1017/S1355838298980542 1:CAS:528:DyaK1cXlvFWkurw%3D 9740133 (Pubitemid 28413461)
    • (1998) RNA , vol.4 , Issue.9 , pp. 1165-1175
    • Burke, D.H.1    Willis, J.H.2
  • 5
    • 33746531165 scopus 로고    scopus 로고
    • Calcium(II)-dependent catalytic activity of the Azoarcus ribozyme: Testing the limits of resolution for in vitro selection
    • DOI 10.1016/j.biochi.2006.01.010, PII S0300908406000113
    • AS Burton N Lehman 2006 Calcium(II)-dependent catalytic activity of the Azoarcus ribozyme: testing the limits of resolution for in vitro selection Biochimie 88 819 825 10.1016/j.biochi.2006.01.010 1:CAS:528:DC%2BD28Xns12ksr4%3D 16494986 (Pubitemid 44142166)
    • (2006) Biochimie , vol.88 , Issue.7 , pp. 819-825
    • Burton, A.S.1    Lehman, N.2
  • 6
    • 64249122566 scopus 로고    scopus 로고
    • Gel purification of radiolabeled nucleic acids via phosphorimaging: Dip-N-Dot
    • 10.1016/j.ab.2009.02.010 1:CAS:528:DC%2BD1MXkvVWnsb4%3D 19232314
    • AS Burton RA Madix N Vaidya CA Riley EJ Hayden A Chepetan C Dìaz Arenas BC Larson N Lehman 2009 Gel purification of radiolabeled nucleic acids via phosphorimaging: Dip-N-Dot Anal Biochem 388 351 352 10.1016/j.ab.2009.02.010 1:CAS:528:DC%2BD1MXkvVWnsb4%3D 19232314
    • (2009) Anal Biochem , vol.388 , pp. 351-352
    • Burton, A.S.1    Madix, R.A.2    Vaidya, N.3    Riley, C.A.4    Hayden, E.J.5    Chepetan, A.6    Arenas, C.7    Larson, B.C.8    Lehman, N.9
  • 7
    • 85045502002 scopus 로고
    • Randomization of genes by PCR mutagenesis
    • 1:CAS:528:DyaK3sXhs1Sr 1490172
    • RC Cadwell GF Joyce 1992 Randomization of genes by PCR mutagenesis PCR Methods Appl 2 28 33 1:CAS:528:DyaK3sXhs1Sr 1490172
    • (1992) PCR Methods Appl , vol.2 , pp. 28-33
    • Cadwell, R.C.1    Joyce, G.F.2
  • 8
    • 52649113859 scopus 로고    scopus 로고
    • Tetrahymena thermophila and Candida albicans group i intron-derived ribozymes can catalyze the trans-excision-splicing reaction
    • 10.1093/nar/gkn507 1:CAS:528:DC%2BD1cXhtFWhsL7O 18684993
    • PP Dotson AK Johnson SM Testa 2008 Tetrahymena thermophila and Candida albicans group I intron-derived ribozymes can catalyze the trans-excision- splicing reaction Nucleic Acids Res 36 5281 5289 10.1093/nar/gkn507 1:CAS:528:DC%2BD1cXhtFWhsL7O 18684993
    • (2008) Nucleic Acids Res , vol.36 , pp. 5281-5289
    • Dotson, P.P.1    Johnson, A.K.2    Testa, S.M.3
  • 9
    • 0015133749 scopus 로고
    • Selforganization of matter and the evolution of biological macromolecules
    • 10.1007/BF00623322 1:CAS:528:DyaE38XjtFaksw%3D%3D 4942363
    • M Eigen 1971 Selforganization of matter and the evolution of biological macromolecules Naturwissenschaften 58 465 523 10.1007/BF00623322 1:CAS:528:DyaE38XjtFaksw%3D%3D 4942363
    • (1971) Naturwissenschaften , vol.58 , pp. 465-523
    • Eigen, M.1
  • 10
    • 0023754962 scopus 로고
    • Gene synthesis
    • 1:CAS:528:DyaL1MXpsFKnug%3D%3D 3140610
    • J Engels E Uhlmann 1988 Gene synthesis Adv Biochem Eng Biotechnol 37 73 127 1:CAS:528:DyaL1MXpsFKnug%3D%3D 3140610
    • (1988) Adv Biochem Eng Biotechnol , vol.37 , pp. 73-127
    • Engels, J.1    Uhlmann, E.2
  • 11
    • 0036669327 scopus 로고    scopus 로고
    • Montmorillonite catalysis of 30-50 mer oligonucleotides: Laboratory demonstration of potential steps in the origin of the RNA world
    • DOI 10.1023/A:1020543312109
    • JP Ferris 2002 Montmorillonite catalysis of 30-50 mer oligonucleotides: laboratory demonstration of potential steps in the origin of the RNA world Orig Life Evol Biosph 32 311 332 10.1023/A:1020543312109 1:CAS:528: DC%2BD38Xns1Shu7c%3D 12458736 (Pubitemid 35255172)
    • (2002) Origins of Life and Evolution of the Biosphere , vol.32 , Issue.4 , pp. 311-332
    • Ferris, J.P.1
  • 12
    • 0003604405 scopus 로고    scopus 로고
    • R. Gesteland T.R. Cech J. Atkins (eds). 3 Cold Spring Harbor Press New York
    • Gesteland R, Cech TR, Atkins J (eds) (2006) The RNA World, 3rd edn. Cold Spring Harbor Press, New York
    • (2006) The RNA World
  • 13
    • 36849148382 scopus 로고
    • The RNA world
    • 10.1038/319618a0
    • W Gilbert 1986 The RNA world Nature 319 618 10.1038/319618a0
    • (1986) Nature , vol.319 , pp. 618
    • Gilbert, W.1
  • 14
    • 0020402387 scopus 로고
    • Exaptation-A missing term in the science of form
    • SJ Gould ES Vrba 1982 Exaptation-A missing term in the science of form Paleobiology 8 4 15
    • (1982) Paleobiology , vol.8 , pp. 4-15
    • Gould, S.J.1    Vrba, E.S.2
  • 15
    • 33747378836 scopus 로고    scopus 로고
    • Self-Assembly of a Group I Intron from Inactive Oligonucleotide Fragments
    • DOI 10.1016/j.chembiol.2006.06.014, PII S1074552106002249
    • EJ Hayden N Lehman 2006 Self-assembly of a group I intron from inactive oligonucleotide fragments Chem Biol 13 909 918 10.1016/j.chembiol.2006.06.014 1:CAS:528:DC%2BD28XoslOhu7k%3D 16931340 (Pubitemid 44247779)
    • (2006) Chemistry and Biology , vol.13 , Issue.8 , pp. 909-918
    • Hayden, E.J.1    Lehman, N.2
  • 16
    • 27144523459 scopus 로고    scopus 로고
    • RNA-directed construction of structurally complex and active ligase ribozymes through recombination
    • DOI 10.1261/rna.2125305
    • EJ Hayden CA Riley AS Burton N Lehman 2005 RNA-directed construction of structurally complex and active ligase ribozymes through recombination RNA 11 1678 1687 10.1261/rna.2125305 1:CAS:528:DC%2BD2MXhtF2ktrnE 16177133 (Pubitemid 41505542)
    • (2005) RNA , vol.11 , Issue.11 , pp. 1678-1687
    • Hayden, E.J.1    Riley, C.A.2    Burton, A.S.3    Lehman, N.4
  • 17
    • 0029963656 scopus 로고    scopus 로고
    • A simple method for 3'-labeling of RNA
    • DOI 10.1093/nar/24.21.4360
    • Z Huang J Szostak 1996 A simple method for 3′-labeling of RNA Nucl Acids Res 24 4360 4361 10.1093/nar/24.21.4360 1:CAS:528:DyaK28XntF2ntLY%3D 8932396 (Pubitemid 26377359)
    • (1996) Nucleic Acids Research , vol.24 , Issue.21 , pp. 4360-4361
    • Huang, Z.1    Szostak, J.W.2
  • 18
    • 67651166851 scopus 로고    scopus 로고
    • One RNA aptamer sequence, two structures: A collaborating pair that inhibits AMPA receptors
    • 10.1093/nar/gkp284 1:CAS:528:DC%2BD1MXovVeksb0%3D 19417060
    • Z Huang W Pei Y Han S Jayaseelan A Shekhtman H Shi L Niu 2009 One RNA aptamer sequence, two structures: a collaborating pair that inhibits AMPA receptors Nucl Acids Res 37 4022 4032 10.1093/nar/gkp284 1:CAS:528: DC%2BD1MXovVeksb0%3D 19417060
    • (2009) Nucl Acids Res , vol.37 , pp. 4022-4032
    • Huang, Z.1    Pei, W.2    Han, Y.3    Jayaseelan, S.4    Shekhtman, A.5    Shi, H.6    Niu, L.7
  • 19
    • 0035906978 scopus 로고    scopus 로고
    • RNA-catalyzed RNA polymerization: Accurate and general RNA-templated primer extension
    • DOI 10.1126/science.1060786
    • WK Johnston PJ Unrau MS Lawrence ME Glasner DP Bartel 2001 RNA-catalyzed RNA polymerization: accurate and general RNA-templated primer extension Science 292 1319 1325 10.1126/science.1060786 1:CAS:528:DC%2BD3MXjvVGjsbg%3D 11358999 (Pubitemid 32476210)
    • (2001) Science , vol.292 , Issue.5520 , pp. 1319-1325
    • Johnston, W.K.1    Unrau, P.J.2    Lawrence, M.S.3    Glasner, M.E.4    Bartel, D.P.5
  • 20
    • 0025828122 scopus 로고
    • The rise and fall of the RNA world
    • 1:CAS:528:DyaK3MXltlSgsL0%3D 1712228
    • GF Joyce 1991 The rise and fall of the RNA world New Biol 3 399 407 1:CAS:528:DyaK3MXltlSgsL0%3D 1712228
    • (1991) New Biol , vol.3 , pp. 399-407
    • Joyce, G.F.1
  • 22
    • 0037062978 scopus 로고    scopus 로고
    • The antiquity of RNA-based evolution
    • DOI 10.1038/418214a
    • GF Joyce 2002 The antiquity of RNA-based evolution Nature 418 214 221 10.1038/418214a 1:CAS:528:DC%2BD38XltFGmu7o%3D 12110897 (Pubitemid 34773780)
    • (2002) Nature , vol.418 , Issue.6894 , pp. 214-221
    • Joyce, G.F.1
  • 23
    • 0015392943 scopus 로고
    • Self-organization of molecular systems and evolution of the genetic apparatus
    • 10.1002/anie.197207981 1:CAS:528:DyaE3sXht1OgtA%3D%3D 4628613
    • H Kuhn 1972 Self-organization of molecular systems and evolution of the genetic apparatus Angew Chem Int Ed Engl 11 798 820 10.1002/anie.197207981 1:CAS:528:DyaE3sXht1OgtA%3D%3D 4628613
    • (1972) Angew Chem Int Ed Engl , vol.11 , pp. 798-820
    • Kuhn, H.1
  • 24
    • 0038369787 scopus 로고    scopus 로고
    • A case for the extreme antiquity of recombination
    • DOI 10.1007/s00239-003-2454-1
    • N Lehman 2003 A case for the extreme antiquity of recombination J Mol Evol 56 770 777 10.1007/s00239-003-2454-1 1:CAS:528:DC%2BD3sXkvVeqt74%3D 12911039 (Pubitemid 36549846)
    • (2003) Journal of Molecular Evolution , vol.56 , Issue.6 , pp. 770-777
    • Lehman, N.1
  • 25
    • 56149124653 scopus 로고    scopus 로고
    • A recombination-based model for the origin and early evolution of genetic information
    • 10.1002/cbdv.200890159 1:CAS:528:DC%2BD1cXht1ClsL3F 18816523
    • N Lehman 2008 A recombination-based model for the origin and early evolution of genetic information Chem Biodivers 5 1707 1717 10.1002/cbdv. 200890159 1:CAS:528:DC%2BD1cXht1ClsL3F 18816523
    • (2008) Chem Biodivers , vol.5 , pp. 1707-1717
    • Lehman, N.1
  • 26
    • 0027525989 scopus 로고
    • Evolution in vitro of an RNA enzyme with altered metal dependence
    • DOI 10.1038/361182a0
    • N Lehman GF Joyce 1993 Evolution in vitro of an RNA enzyme with altered metal dependence Nature 361 182 185 10.1038/361182a0 1:CAS:528: DyaK3sXhtF2rsrY%3D 8421526 (Pubitemid 23031357)
    • (1993) Nature , vol.361 , Issue.6408 , pp. 182-185
    • Lehman, N.1    Joyce, G.F.2
  • 27
    • 0037256766 scopus 로고    scopus 로고
    • In search of an RNA replicase ribozyme
    • DOI 10.1016/S1074-5521(03)00003-6
    • KE McGinness GF Joyce 2003 In search of an RNA replicase ribozyme Chem Biol 10 5 14 10.1016/S1074-5521(03)00003-6 1:CAS:528:DC%2BD3sXps1Smtg%3D%3D 12573693 (Pubitemid 36154469)
    • (2003) Chemistry and Biology , vol.10 , Issue.1 , pp. 5-14
    • McGinness, K.E.1    Joyce, G.F.2
  • 28
    • 0022845689 scopus 로고
    • RNA catalysis and the origins of life
    • 10.1016/S0022-5193(86)80149-7 1:CAS:528:DyaL2sXivVyrtQ%3D%3D 2442564
    • LE Orgel 1986 RNA catalysis and the origins of life J Theor Biol 123 127 149 10.1016/S0022-5193(86)80149-7 1:CAS:528:DyaL2sXivVyrtQ%3D%3D 2442564
    • (1986) J Theor Biol , vol.123 , pp. 127-149
    • Orgel, L.E.1
  • 29
    • 0032563297 scopus 로고    scopus 로고
    • Folding intermediates of a self-splicing RNA: Mispairing of the catalytic core
    • DOI 10.1006/jmbi.1998.1901
    • J Pan SA Woodson 1998 Folding intermediates of a self-splicing RNA: mispairing of the catalytic core J Mol Biol 280 597 609 10.1006/jmbi.1998.1901 1:CAS:528:DyaK1cXlt1eht7k%3D 9677291 (Pubitemid 28336368)
    • (1998) Journal of Molecular Biology , vol.280 , Issue.4 , pp. 597-609
    • Pan, J.1    Woodson, S.A.2
  • 30
    • 0034635353 scopus 로고    scopus 로고
    • Fast folding of a ribozyme by stabilizing core interactions: Evidence for multiple folding pathways in RNA
    • 10.1006/jmbi.1999.3439 1:CAS:528:DC%2BD3cXotlSitA%3D%3D 10656822
    • J Pan ML Deras SA Woodson 2000 Fast folding of a ribozyme by stabilizing core interactions: evidence for multiple folding pathways in RNA J Mol Biol 296 133 144 10.1006/jmbi.1999.3439 1:CAS:528:DC%2BD3cXotlSitA%3D%3D 10656822
    • (2000) J Mol Biol , vol.296 , pp. 133-144
    • Pan, J.1    Deras, M.L.2    Woodson, S.A.3
  • 31
    • 0003075565 scopus 로고
    • On the problems of evolution and biochemical information transfer
    • M. Kasha B. Pullmann (eds). Academic Press New York
    • Rich A (1962) On the problems of evolution and biochemical information transfer. In: Kasha M, Pullmann B (eds) Horizons in biochemistry. Academic Press, New York, pp 103-126
    • (1962) Horizons in Biochemistry , pp. 103-126
    • Rich, A.1
  • 32
    • 0348136386 scopus 로고    scopus 로고
    • Generalized RNA-directed recombination of RNA
    • DOI 10.1016/j.chembiol.2003.11.015
    • CA Riley N Lehman 2003 Generalized RNA-directed recombination of RNA Chem Biol 10 1233 1243 10.1016/j.chembiol.2003.11.015 1:CAS:528:DC%2BD3sXhtVWis73M 14700631 (Pubitemid 38042727)
    • (2003) Chemistry and Biology , vol.10 , Issue.12 , pp. 1233-1243
    • Riley, C.A.1    Lehman, N.2
  • 33
    • 23144436036 scopus 로고    scopus 로고
    • Assembly PCR oligo maker: A tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production
    • DOI 10.1093/nar/gki380
    • R Rydzanicz XS Zhao PE Johnson 2005 Assembly PCR oligo maker: a tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production Nucleic Acids Res 33 W521 W525 10.1093/nar/gki380 1:CAS:528:DC%2BD2MXlslyrtb0%3D 15980526 (Pubitemid 44529975)
    • (2005) Nucleic Acids Research , vol.33 , Issue.WEB. SERV. ISS.
    • Rydzanicz, R.1    Zhao, X.S.2    Johnson, P.E.3
  • 34
    • 0033485559 scopus 로고    scopus 로고
    • Non-unity molecular heritability demonstrated by continuous evolution in vitro
    • 10.1016/S1074-5521(00)80005-8 1:CAS:528:DyaK1MXotVygsrw%3D 10631514
    • T Schmitt N Lehman 1999 Non-unity molecular heritability demonstrated by continuous evolution in vitro Chem Biol 6 857 869 10.1016/S1074-5521(00)80005-8 1:CAS:528:DyaK1MXotVygsrw%3D 10631514
    • (1999) Chem Biol , vol.6 , pp. 857-869
    • Schmitt, T.1    Lehman, N.2
  • 35
    • 0032549780 scopus 로고    scopus 로고
    • RNA folding at millisecond intervals by synchrotron hydroxyl radical footprinting
    • DOI 10.1126/science.279.5358.1940
    • B Sclavi M Sullivan MR Chance M Brenowitz SA Woodson 1998 RNA folding at millisecond intervals by synchrotron hydroxyl radical footprinting Science 279 1940 1943 10.1126/science.279.5358.1940 1:CAS:528:DyaK1cXhvFKnu7g%3D 9506944 (Pubitemid 28168649)
    • (1998) Science , vol.279 , Issue.5358 , pp. 1940-1943
    • Sclavi, B.1    Sullivan, M.2    Chance, M.R.3    Brenowitz, M.4    Woodson, S.A.5
  • 36
    • 33344468482 scopus 로고    scopus 로고
    • Frequency of RNA-RNA interaction in a model of the RNA World
    • DOI 10.1261/rna.2204506
    • JC Striggles MB Martin FJ Schmidt 2006 Frequency of RNA-RNA interaction in a model of the RNA World RNA 12 353 359 10.1261/rna.2204506 1:CAS:528:DC%2BD28Xit1Kmsb0%3D 16495233 (Pubitemid 43290864)
    • (2006) RNA , vol.12 , Issue.3 , pp. 353-359
    • Striggles, J.C.1    Martin, M.B.2    Schmidt, F.J.3
  • 37
    • 0001878781 scopus 로고
    • In vitro selection of functional RNA sequences
    • R. Gesteland J. Atkins (eds). 1 Cold Spring Harbor Press New York
    • Szostak J, Ellington A (1993) In vitro selection of functional RNA sequences. In: Gesteland R, Atkins J (eds) The RNA world, 1st edn. Cold Spring Harbor Press, New York, pp 511-533
    • (1993) The RNA World , pp. 511-533
    • Szostak, J.1    Ellington, A.2
  • 38
    • 0035366896 scopus 로고    scopus 로고
    • Beyond kinetic traps in RNA folding
    • DOI 10.1016/S0959-440X(00)00206-2
    • DK Treiber JR Williamson 2001 Beyond kinetic traps in RNA folding Curr Opin Struct Biol 11 309 314 10.1016/S0959-440X(00)00206-2 1:CAS:528: DC%2BD3MXks1Gqs7k%3D 11406379 (Pubitemid 32568270)
    • (2001) Current Opinion in Structural Biology , vol.11 , Issue.3 , pp. 309-314
    • Treiber, D.K.1    Williamson, J.R.2
  • 39
    • 0029999325 scopus 로고    scopus 로고
    • Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions
    • DOI 10.1093/nar/24.14.2627
    • JP Vartanian M Henry S Wain-Hobson 1996 Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions Nucleic Acids Res 24 2627 2631 10.1093/nar/24.14.2627 1:CAS:528:DyaK28XkslWmurw%3D 8758987 (Pubitemid 26239725)
    • (1996) Nucleic Acids Research , vol.24 , Issue.14 , pp. 2627-2631
    • Vartanian, J.-P.1    Henry, M.2    Wain-Hobson, S.3
  • 40
    • 34250860849 scopus 로고    scopus 로고
    • Selection of an improved RNA polymerase ribozyme with superior extension and fidelity
    • DOI 10.1261/rna.548807
    • HS Zaher PJ Unrau 2007 Selection of an improved RNA polymerase ribozyme with superior extension and fidelity RNA 13 1017 1026 10.1261/rna.548807 1:CAS:528:DC%2BD2sXnsFSqtb4%3D 17586759 (Pubitemid 46984892)
    • (2007) RNA , vol.13 , Issue.7 , pp. 1017-1026
    • Zaher, H.S.1    Unrau, P.J.2
  • 41
    • 0027991626 scopus 로고
    • Kinetic intermediates in RNA folding
    • 10.1126/science.8052848 1:CAS:528:DyaK2cXmslClsLk%3D 8052848
    • P Zarrinkar JR Williamson 1994 Kinetic intermediates in RNA folding Science 265 918 924 10.1126/science.8052848 1:CAS:528:DyaK2cXmslClsLk%3D 8052848
    • (1994) Science , vol.265 , pp. 918-924
    • Zarrinkar, P.1    Williamson, J.R.2
  • 42
    • 11144256451 scopus 로고    scopus 로고
    • Fast formation of the P3-P7 pseudoknot: A strategy for efficient folding of the catalytically active ribozyme
    • DOI 10.1261/rna.7145105
    • L Zhang M Xiao C Lu Y Zhang 2005 Fast formation of the P3-P7 pseudoknot: a strategy for efficient folding of the catalytically active ribozyme RNA 11 59 69 10.1261/rna.7145105 1:CAS:528:DC%2BD2MXhvFaktg%3D%3D 15574515 (Pubitemid 40041573)
    • (2005) RNA , vol.11 , Issue.1 , pp. 59-69
    • Zhang, L.1    Xiao, M.2    Lu, C.3    Zhang, Y.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.