Tetrahymena thermophila and Candida albicans Group I intron-derived ribozymes can catalyze the trans-excision-splicing reaction

Nucleic Acids Research

Volumn 36, Issue 16, 2008, Pages 5281-5289

  Dotson, P Patrick ^a   Johnson, Ashley K ^a   Testa, Stephen M ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE; NUCLEOTIDE; RIBOZYME;

ARTICLE; CANDIDA ALBICANS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME SUBSTRATE; INTRON; MOLECULAR INTERACTION; NONHUMAN; PNEUMOCYSTIS CARINII; PRIORITY JOURNAL; SPECIES COMPARISON; TETRAHYMENA THERMOPHILA; TRANS EXCISION SPLICING; TRANS SPLICING;

ANIMALS; CANDIDA ALBICANS; CATALYSIS; GUANOSINE; INTRONS; MOLECULAR WEIGHT; RNA, CATALYTIC; SEQUENCE ANALYSIS, RNA; TETRAHYMENA THERMOPHILA; TRANS-SPLICING;

CANDIDA; CANDIDA ALBICANS; KOBUS; PNEUMOCYSTIS; PNEUMOCYSTIS CARINII; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

EID: 52649113859     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkn507     Document Type: Article

References (27)

1. Cech,T.R. (1990) Self-splicing of group I introns. Annu. Rev. Biochem., 59, 543-568.
2. Dotson,P.P. II and Testa,S.M. (2006) Group I intron-derived ribozyme recombination reactions. Rec. Develop. Nucleic Acids Res., 2 307-324.
3. Bell,M.A., Johnson,A.J. and Testa,S.M. (2002) Ribozyme-catalyzed excision of targeted sequences from within RNAs. Biochemistry, 41, 15327-15333.
4. Dotson,P.P. II, Sinha,J. and Testa,S.M. (2008) A Pneumocystis carinii group I intron-derived ribozyme utilizes an endogenous guanosine as the first reaction step nucleophile in the trans excision-splicing reaction. Biochemistry, 47, 4780-4787.
5. Zaug,A.J., Grosshans,C.A. and Cech,T.R. (1988) Sequence-specific endoribonuclease activity of the Tetrahymena ribozyme: Enhanced cleavage of certain oligonucleotide substrates that form mismatched ribozyme-substrate complexes. Biochemistry, 27, 8924-8931.
6. Disney,M.D., Haidaris,C.G. and Turner,D.H. (2001) Recognition elements for 5′ exon substrate binding to the Candida albicans group I intron. Biochemistry, 40, 6507-6519.
7. Testa,S.M., Haidaris,C.G., Gigliotti,F. and Turner,D.H. (1997) A Pneumocystis carinii group I intron ribozyme that does not require 2′ OH groups on its 5′ exon mimic for binding to the catalytic core. Biochemistry, 36, 15303-15314.
8. Johnson,A.J., Sinha,J. and Testa,S.M. (2005) Trans insertion-splicing: Ribozyme-catalyzed insertion of targeted sequences into RNAs. Biochemistry, 44, 10702-10710.
9. Bell,M.A., Sinha,J., Johnson,A.J. and Testa,S.M. (2004) Enhancing the second step of the trans excision-splicing reaction of a group I ribozyme by exploiting P9.0 and P10 for intermolecular recognition. Biochemistry, 43, 4323-4331.
10. Baum,D.A., Sinha,J. and Testa,S.M. (2005) Molecular recognition in a trans excision-splicing ribozyme: Non-Watson-Crick base pairs at the 5′ splice site and ωG at the 3′ splice site can play a role in determining the binding register of reaction substrates. Biochemistry 44, 1067-1077.
11. Milligan,J.F., Groebe,D.R., Witherell,G.W. and Uhlenbeek,O.C. (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res., 15, 8783-8798.
12. Woodson,S.A. and Cech,T.R. (1989) Reverse self-splicing of the Tetrahymena group I intron: Implication for the directionality of splicing and for intron transposition. Cell, 57, 335-345.
13. Roman,J. and Woodson,S.A. (1998) Integration of the Tetrahymena group I intron into bacterial rRNA by reverse splicing in vivo. Proc. Natl Acad. Sci. USA., 95, 2134-2139.
14. Inoue,T., Sullivan,F.X. and Cech,T.R. (1986) New reactions of the ribosomal RNA precursor of Tetrahymena and the mechanism of self-splicing. J. Mol. Biol., 189, 143-165.
15. Herschlag,D. and Cech,T.R. (1990) Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. 1. Kinetic description of the reaction of an RNA substrate complementary to the active site. Biochemistry, 29, 10159-10171.
16. Zaug,A.J., Davila-Aponte,J.A. and Cech,T.R. (1994) Catalysis of RNA cleavage by a ribozyme derived from the group I intron of Anabaena pre-tRNA(Leu). Biochemistry, 33, 14935-14947.
17. Brehm,S.L. and Cech,T.R. (1983) Fate of an intervening sequence ribonucleic acid: Excision and cyclization of the Tetrahymena ribosomal ribonucleic acid intervening sequence in vivo. Biochemistry 22, 2390-2397.
18. Zaug,A.J., Grabowski,P.J. and Cech,T.R. (1983) Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage-ligation reaction. Nature, 301, 578-583.
19. Zaug,A.J., Kent,J.R. and Cech,T.R. (1984) A labile phosphodiester bond at the ligation junction in a circular intervening sequence RNA. Science, 224, 574-578.
20. Zaug,A.J., Kent,J.R. and Cech,T.R. (1985) Reactions of the intervening sequence of the Tetrahymena ribosomal ribonucleic acid precursor pH dependence of cyclization and site-specific hydrolysis. Biochemistry, 24, 6211-6218.
21. Zaug,A.J. and Cech,T.R. (1986) The Tetrahymena intervening sequence ribonucleic acid enzyme is a phosphotransferase and an acid phosphatase. Biochemistry, 25, 4478-4482.
22. Zaug,A.J. and Cech,T.R. (1986) The intervening sequence RNA of Tetrahymena is an enzyme. Science, 231, 470-475.
23. Chowrira,B.M., Berzel-Herranz,A. and Burke,J.M. (1993) Novel RNA polymerization reaction catalyzed by a group I intron-derived ribozyme. EMBO J., 12, 3599-3605.
24. Mei,R. and Herschlag,D. (1996) Mechanistic investigations of a ribozyme derived from the Tetrahymena group I intron: Insights into catalysis and the second step of self-splicing. Biochemistry, 35, 5796-5809.
25. Riley,C. A. and Lehman,N. (2003) Generalized RNA-directed recombination of RNA. Chem. Biol., 10, 1233-1243.
26. Dotson,P.P. II, Sinha,J. and Testa,S.M. (2008) Kinetic characterization of the first reaction step of the ribozyme-catalyzed trans excision-splicing reaction. FEBS J., 275, 3110-3122.
27. Sargueil,B. and Tanner,N.K. (1993) A shortened form of the Tetrahymena thermophila group I intron can catalyze the complete self-splicing reaction in trans. J. Mol. Biol., 233, 629-643.