Tetra-glutamic acid residues adjacent to Lys248 in HMG-CoA reductase are critical for the ubiquitination mediated by gp78 and UBE2G2

Acta Biochimica et Biophysica Sinica

Volumn 42, Issue 5, 2010, Pages 303-310

  Miao, Honghua ^a   Jiang, Wei ^a,b   Ge, Liang ^a   Li, Boliang ^a   Song, Baoliang ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b LANZHOU UNIVERSITY   (China)

Author keywords

Endoplasmic reticulum associated protein degradation (ERAD); Gp78; HMG CoA reductase; Tetra glutamic acid residue; UBE2G2

Indexed keywords

3 HYDROXY 3 METHYLGLUTARYL COENZYME A; 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A; ACYL COENZYME A; AUTOCRINE MOTILITY FACTOR RECEPTOR; CYTOKINE RECEPTOR; GLUTAMIC ACID; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LYSINE; UBE2G2 PROTEIN, HUMAN; UBIQUITIN CONJUGATING ENZYME; UBIQUITIN PROTEIN LIGASE;

ANIMAL; ARTICLE; CHEMISTRY; CHO CELL; CRICETULUS; HAMSTER; HUMAN; METABOLISM; PHYSIOLOGY; UBIQUITINATION;

ACYL COENZYME A; ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; GLUTAMIC ACID; HUMANS; HYDROXYMETHYLGLUTARYL COA REDUCTASES; LYSINE; RECEPTORS, CYTOKINE; UBIQUITIN-CONJUGATING ENZYMES; UBIQUITIN-PROTEIN LIGASES; UBIQUITINATION;

TETRA;

EID: 77951849191     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1093/abbs/gmq022     Document Type: Article

References (21)

1. Goldstein JL and Brown MS. Regulation of the mevalonate pathway. Nature 1990, 343: 425-430.
2. Roitelman J, Olender EH, Bar-Nun S, Dunn WA, Jr and Simoni RD. Immunological evidence for eight spans in the membrane domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase: implications for enzyme degradation in the endoplasmic reticulum. J Cell Biol 1992, 117: 959-973.
3. Gil G, Faust JR, Chin DJ, Goldstein JL and Brown MS. Membrane-bound domain of HMG CoA reductase is required for sterol-enhanced degradation of the enzyme. Cell 1985, 41: 249-258.
4. Ravid T, Doolman R, Avner R, Harats D and Roitelman J. The ubiquitin-proteasome pathway mediates the regulated degradation of mammalian 3-hydroxy-3-methylglutaryl-coenzyme A reductase. J Biol Chem 2000, 275: 35840-35847.
5. Sever N, Song BL, Yabe D, Goldstein JL, Brown MS and DeBose-Boyd RA. Insig-dependent ubiquitination and degradation of mammalian 3-hydroxy-3- methylglutaryl-CoA reductase stimulated by sterols and gera-nylgeraniol. J Biol Chem 2003, 278: 52479-52490.
6. Song BL, Javitt NB and DeBose-Boyd RA. Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol. Cell Metab 2005, 1: 179-189.
7. Song BL and DeBose-Boyd RA. Ubiquitination of 3-hydroxy-3-methylglutaryl- CoA reductase in permeabilized cells mediated by cytosolic E1 and a putative membrane-bound ubiquitin ligase. J Biol Chem 2004, 279: 28798-28806.
8. Song BL, Sever N and DeBose-Boyd RA. Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubi-quitination to degradation of HMG CoA reductase. Mol Cell 2005, 19: 829-840.
9. Cao J, Wang J, Qi W, Miao HH, Wang J, Ge L and DeBose-Boyd RA, et al. Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by regulating the stability of HMG-CoA reductase. Cell metab 2007, 6: 115-128.
10. Kita T, Brown MS and Goldstein JL. Feedback regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in livers of mice treated with mevinolin, a competitive inhibitor of the reductase. J Clin Invest 1980, 66: 1094-1100.
11. Goldstein JL, Kita T and Brown MS. Defective lipoprotein receptors and atherosclerosis. Lessons from an animal counterpart of familial hypercho-lesterolemia. N Engl J Med 1983, 309: 288-296.
12. Sever N, Yang T, Brown MS, Goldstein JL and DeBose-Boyd RA. Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain. Mol cell 2003, 11: 25-33.
13. Sakai J, Nohturfft A, Cheng D, Ho YK, Brown MS and Goldstein JL. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein. Biol Chem 1997, 272: 20213-20221.
14. Liscum L, Luskey KL, Chin DJ, Ho YK, Goldstein JL and Brown MS. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe. J Biol Chem 1983, 258: 8450-8455.
15. Rawson RB, DeBose-Boyd R, Goldstein JL and Brown MS. Failure to cleave sterol regulatory element-binding proteins (SREBPs) causes cholesterol auxotrophy in Chinese hamster ovary cells with genetic absence of SREBP cleavage-activating protein. J Biol Chem 1999, 274: 28549-28556.
16. Gardner RG and Hampton RY. A 'distributed degron' allows regulated entry into the ER degradation pathway. EMBO J. 1999, 18: 5994-6004.
17. Yang T, Espenshade PJ, Wright ME, Yabe D, Gong Y, Aebersold R and Goldstein JL, et al. Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER. Cell 2002, 110: 489-500.
18. Yabe D, Brown MS and Goldstein JL. Insig-2, a second endoplasmic reti-culum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins. Proc Natl Acad Sci USA 2002, 99: 12753-12758.
19. Fang S, Ferrone M, Yang C, Jensen JP, Tiwari S and Weissman AM. The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. Proc Natl Acad Sci USA 2001, 98: 14422-14427.
20. Catic A, Collins C, Church GM and Ploegh HL. Preferred in vivo ubiquiti-nation sites. Bioinformatics 2004, 20: 3302-3307.
21. Jonassen I, Collins JF and Higgins DG. Finding flexible patterns in unaligned protein sequences. Protein Sci 1995, 4: 1587-1595.