Isolation of cytotoxic T cell and NK granules and purification of their effector proteins

Current Protocols in Cell Biology

Volumn , Issue SUPPL. 47, 2010, Pages

  Thiery, Jerome ^a,b   Walch, Michael ^a,b   Jensen, Danielle K ^a,b   Martinvalet, Denis ^a,b   Lieberman, Judy ^a,b  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

CTL; cytotoxic granules; granulysin; granzyme; NK cells; perforin

Indexed keywords

ALPHA GLUCOSIDASE; BETA GLUCOSIDASE; CALRETICULIN; CATHEPSIN B; CATHEPSIN D; CD63 ANTIGEN; CELL PROTEIN; CHROMIUM; DIPEPTIDYL PEPTIDASE I; GRANULYSIN; GRANZYME A; GRANZYME B; HYDROGEN POTASSIUM ADENOSINE TRIPHOSPHATASE; LIPOCORTIN 5; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 1; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 2; NITROGEN; PERFORIN; PROPIDIUM IODIDE; SERGLYCIN; UNCLASSIFIED DRUG;

APOPTOSIS; CELL DISRUPTION; CELL FRACTIONATION; CELL GRANULE; CELL GRANULE ISOLATION; COLONY FORMING UNIT; CYTOTOXIC T LYMPHOCYTE; CYTOTOXICITY; DENSITY GRADIENT CENTRIFUGATION; FAST PROTEIN LIQUID CHROMATOGRAPHY; FLOW CYTOMETRY; HUMAN; IMMUNOHISTOCHEMISTRY; NATURAL KILLER T CELL; NONHUMAN; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVIEW; TURBIDIMETRY;

ANIMALS; CELL FRACTIONATION; CELL SEPARATION; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; CHROMATOGRAPHY, LIQUID; CYTOPLASMIC GRANULES; HUMANS; KILLER CELLS, NATURAL; PROTEINS; RATS; T-LYMPHOCYTES, CYTOTOXIC;

EID: 77951688913     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb0337s47     Document Type: Review

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