Design and characterisation of a miniature stirred bioreactor system for parallel microbial fermentations

Biochemical Engineering Journal

Volumn 39, Issue 1, 2008, Pages 164-176

  Gill, N K ^a   Appleton, M ^b   Baganz, F ^a   Lye, G J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b Bioxplore   (United Kingdom)

Author keywords

Fermentation; Miniature bioreactor; On line monitoring; Parallel operation

Indexed keywords

BIOMASS; DISSOLVED OXYGEN; FERMENTATION; MICROBIOLOGY; ONLINE SYSTEMS; PH EFFECTS;

MINIATURE BIOREACTORS; ONLINE MONITORING; PARALLEL OPERATION;

BIOREACTORS;

DISSOLVED OXYGEN;

AEROBIC FERMENTATION; AGITATION; ARTICLE; BACTERIAL GROWTH; BIOMASS; ESCHERICHIA COLI; KINETICS; NONHUMAN; OPTICAL DENSITY; PH; PRIORITY JOURNAL; REPRODUCIBILITY; STIRRED REACTOR;

EID: 42749084214     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2007.09.001     Document Type: Article

References (34)

1. Hibbert E.G., Baganz F., Hailes H.C., Ward J.M., Lye G.J., Woodley J.M., and Dalby P.A. Directed evolution of biocatalytic processes. Biomol. Eng. 22 (2005) 11-19
2. Doig S.D., Baganz F., and Lye G.J. High throughput screening and process optimisation. In: Ratledge C., and Kristiansen B. (Eds). Basic Biotechnology. third ed. (2006), Cambridge University Press, UK
3. Lye G.J., Ayazi-Shamlou P., Baganz F., Dalby P.A., and Woodley J.M. Accelerated design of bioconversion processes using automated microscale processing techniques. Trends Biotechnol. 21 (2003) 29-37
4. Kumar S., Wittman C., and Heinzle E. Minibioreactors Biotechnol. Lett. 26 (2004) 1-10
5. Weuster-Botz D. Parallel reactor systems for bioprocess development. Adv. Biochem. Eng Biotechnol. 92 (2005) 125-143
6. Fernandes P., and Cabral J.M.S. Review: microlitre/millilitre shaken bioreactors in fermentative and biotransformation processes. Biocatal. Biotransform. 24 (2006) 237-252
7. Betts J.I., and Baganz F. Review: miniature bioreactors: current practices and future opportunities. Microb. Cell Factories 5 (2006) 21
8. Dutez W.A., Ruedi L., Hermann R., O'Conner K., Büchs J., and Witholt B. Methods for intense aeration, growth, storeage and replication of bacterial strains in microtiter plates. Appl. Env. Microbiol. 66 (2000) 2641-2646
9. Elmahdi I., Baganz F., Dixon K., Harrop T., Sugden D., and Lye G.J. pH control in microwell fermentations of S. erythraea CA340: influence on biomass growth kinetics and erythromycin biosynthesis. Biochem. Eng. J. 16 (2003) 299-310
10. John G.T., Klimant I., Wittmann C., and Heinzle E. Integrated optical sensing of dissolved oxygen in microtitre plates: a novel tool for microbial cultivation. Biotechnol. Bioeng. 81 (2003) 829-836
11. Rege K., Pepsin M., Falcon B., Steele L., and Heng M. High-throughput process development for recombinant protein purification. Biotechnol. Bioeng. 93 (2006) 618-630
12. Jackson N.B., Liddell J.M., and Lye G.J. An automated microscale technique for the quantitative and parallel analysis of microfiltration operations. J. Membrane Sci. 276 (2006) 31-41
13. Ferreira-Torres C., Micheletti M., and Lye G.J. Microscale process evaluation of recombinant biocatalyst libraries: application to Baeyer-Villiger mono-oxygenase catalysed lactone synthesis. Bioprocess Biosystems Eng. 28 (2005) 83-93
14. Micheletti M., Barrett T., Doig S.D., Baganz F., Levy M.S., Woodley J.M., and Lye G.J. Fluid mixing in shaken bioreactors: implications for scale-up predictions from microlitre scale microbial and mammalian cell cultures. Chem. Eng. Sci. 61 (2006) 2939-2949
15. Doig S.D., O'Sullivan L.M., Patel S., Ward J.M., and Woodley J.M. Large scale production of cyclohexanone monooxygenase from Escherichia coli TOP10 pQR239. Enzyme Microb. Technol. 28 (2001) 265-274
16. Doig S.D., Diep A., and Baganz F. Characterisation of a novel miniature bubble column bioreactor for high throughput cell cultivation. Biochem. Eng. J. 23 (2004) 97-105
17. Van Loo M.E., and Lengowski P.E. Automated workstations for parallel synthesis. Org. Process Res. Dev. 6 (2002) 833-840
18. van't Riet K. Review of measuring methods and results in non-viscous gas liquid mass transfer in stirred vessels. Ind. Eng. Chem. Process Design Dev. 18 (1979) 357-364
19. Dunn I.J., and Einsele A.J. Oxygen transfer coefficients by the dynamic method. J. Appl. Chem. Biotechnol. 25 (1975) 707-720
20. Atkinson B., and Mavituna F. Biochemical Engineering and Biotechnology Handbook. second ed. (1999), Stockton press
21. Bailey, and Ollis. Biochemical Engineering Fundamentals (1986), McGraw-Hill International, Singapore
22. Morao A., Maia C.I., Fonseca M.M.R., Vasconcelos J.M.T., and Alves S.S. Effect of antifoam addition on gas-liquid mass transfer in stirred fermenters. Bioprocess Eng. 20 (1999) 165-172
23. Puskeiler R., Kaufmann K., and Weuster-Botz D. Development, parallelisation, and automation of a gas inducing millilitre-scale bioreactor for high-throughput bioprocess design (HTBD). Biotechnol. Bioeng. 89 (2005) 512-523
24. Puskeiler R., Kusterer A., John G.T., and Weuster-Botz D. Miniature bioreactors for automated high-throughput bioprocess design (HTBD): Reproducibility of parallel fed-batch cultivations with Escherichia coli. Biotechnol. Appl. Biochem. 42 (2005) 227-235
25. Betts J.I., Doig S.D., and Baganz F. Charaterisation and application of a miniature 10 mL stirred-tank bioreactor, showing scale-down equivalence with a conventional 7 L reactor. Biotechnol. Prog. 22 (2006) 681-688
26. Lamping S.R., Zhang H., Allen B., and Ayazi-Shamlou P. Design of a prototype miniature bioreactor for high throughput automated bioprocessing. Chem. Eng. Sci. 58 (2003) 747-758
27. Kostov Y., Harms P., Randers-Eichhorn L., and Rao G. Low-cost microbioreactor for high-throughput bioprocessing. Biotechnol. Bioeng. 72 (2001) 346-352
28. Harms P., Kostov Y., French J.A., Soliman M., Anjanappa M., Ram A., and Rao G. Design and performance of a 24-station high throughput microbioreactor. Biotechnol. Bioeng 93 (2006) 6-13
29. Dilsen S., Paul W., Herforth D., Sandgathe A., Altenbach-Rehm J., Freudl R., Wandrey C., and Weuster-Botz D. Evaluation of parallel operated small-scale bubble columns for microbial process development using Staphylococcus carnosus. J. Biotechnol. 88 (2001) 77-84
30. Weuster-Botz D., Altenbach-Rehm J., and Hawrylenko A. Process-engineering characterisation of small-scale bubble columns for microbial process development. Bioprocess Biosystems Eng. 24 (2001) 3-11
31. La. Biotechnol. Prog. 21 (2005) 1175-1182
32. Anderlei T., and Büchs J. Device for sterile online measurement of the oxygen transfer rate in shaking flasks. Biochem. Eng. J. 7 (2001) 157-162
33. Weuster-Botz D., Altenbach-Rehm J., and Arnold M. Parallel substrate feeding and pH control in shaking flasks. Biochem. Eng. J. 7 (2001) 163-170
34. Knorr B., Schlieker H., Hohmann H.P., and Weuster-Botz D. Scale-down and parallel operation of the riboflavin production process with Bacillus subtilis. Biochem. Eng. J. 33 (2007) 263-274