Effect of prolonged exposure to organic solvents on the active site environment of subtilisin Carlsberg

Journal of Molecular Catalysis B: Enzymatic

Volumn 64, Issue 1-2, 2010, Pages 38-44

  Bansal, Vibha ^a,d   Delgado, Yamixa ^a   Fasoli, Ezio ^a   Ferrer, Amaris ^a   Griebenow, Kai ^b   Secundo, Francesco ^c   Barletta, Gabriel L ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^b UNIVERSITY OF PUERTO RICO RIO PIEDRAS   (Puerto Rico)

^c CNR   (Italy)

^d UNIVERSITY OF PUERTO RICO AT CAYEY   (Puerto Rico)

Author keywords

Active site polarity; Enzyme catalysis in organic solvents; Enzyme storage stability in organic solvents; Subtilisin Carlsberg

Indexed keywords

ACTIVE-SITE POLARITY; ENZYME CATALYSIS; ENZYME CATALYSIS IN ORGANIC SOLVENTS; STORAGE STABILITY; SUBTILISIN CARLSBERG;

ACETONITRILE; AMINO ACIDS; CATALYSIS; DISSOLUTION; ENZYME ACTIVITY; ENZYMES; ETHERS; FLUOROPHORES; GENE ENCODING; PHOSPHATASES; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; SPECTROSCOPIC ANALYSIS; SYNTHESIS (CHEMICAL);

ORGANIC SOLVENTS;

ACETONITRILE; DIOXANE; MACROGOL; ORGANIC SOLVENT; SUBTILISIN; SUBTILISIN CARLSBERG; UNCLASSIFIED DRUG; WATER;

ARTICLE; CHEMICAL MODIFICATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE SPECTROSCOPY; LONG TERM EXPOSURE; MOLECULAR DYNAMICS;

EID: 77951499608     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2010.01.021     Document Type: Article

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