Active-site motions and polarity enhance catalytic turnover of hydrated subtilisin dissolved in organic solvents

Journal of the American Chemical Society

Volumn 131, Issue 12, 2009, Pages 4294-4300

  Hudson, Elton P ^a   Eppler, Ross K ^a   Beaudoin, Julianne M ^a   Dordick, Jonathan S ^b   Reimer, Jeffrey A ^a   Clark, Douglas S ^a  

^a University of California   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC TURNOVER; DIELECTRIC CONSTANTS; ELECTRONIC ENVIRONMENTS; ELECTROSTATIC EFFECT; EMPIRICAL MEASURE; NMR RELAXATION EXPERIMENTS; SENSITIVE PROBE; SLOW MOTION; SUBTILISIN; SUBTILISIN CARLSBERG; TETRA-HYDROFURAN; TRANSITION STATE; TURNOVER NUMBER; WATER ACTIVITY;

CATALYSIS; DISSOLUTION; HYDRATES; NUCLEAR MAGNETIC RESONANCE; ORGANIC SOLVENTS; PARAFFINS; SURFACE ACTIVE AGENTS;

HYDRATION;

ORGANIC SOLVENT; SUBTILISIN; TETRAHYDROFURAN; WATER; SOLVENT; SURFACTANT;

ARTICLE; CATALYSIS; DIELECTRIC CONSTANT; DIPOLE; ELECTRICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME METABOLISM; FLUORINE NUCLEAR MAGNETIC RESONANCE; HYDRATION; POLARIZATION; PROTON NUCLEAR MAGNETIC RESONANCE; SOLUBILIZATION; SOLVATION; THERMODYNAMICS; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; KINETICS; LIGHT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; ORGANIC CHEMISTRY; PROCEDURES; RADIATION SCATTERING; STATIC ELECTRICITY;

CATALYSIS; CATALYTIC DOMAIN; CHEMISTRY, ORGANIC; KINETICS; LIGHT; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MOLECULAR CONFORMATION; SCATTERING, RADIATION; SOLVENTS; STATIC ELECTRICITY; SUBTILISINS; SURFACE-ACTIVE AGENTS; THERMODYNAMICS;

EID: 67849103981     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja806996q     Document Type: Article

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