Structure-function studies of an engineered scaffold protein derived from stefin A. I: Development of the SQM variant

Protein Engineering, Design and Selection

Volumn 23, Issue 5, 2010, Pages 403-413

  Hoffmann, Toni ^a   Stadler, Lukas Kurt Josef ^a   Busby, Michael ^b   Song, Qifeng ^a   Buxton, Anthony T ^a   Wagner, Simon D ^c   Davis, Jason J ^b   Ko Ferrigno, Paul ^a,d  

^a ST JAMES S UNIVERSITY HOSPITAL   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

^d HUTCHISON MRC RESEARCH CENTRE   (United Kingdom)

Author keywords

Circular dichroism spectroscopy; Epitope recognition; Peptide aptamer; Scaffold protein

Indexed keywords

DETRIMENTAL EFFECTS; HUMAN CELLS; INSERTION SITE; N-TERMINALS; OLIGOPEPTIDES; OPEN READING FRAME; PEPTIDE APTAMER; PROTEASE INHIBITOR; PROTEIN EXPRESSIONS; PROTEIN-PROTEIN INTERACTIONS; SCAFFOLD PROTEIN; STEP-BY-STEP APPROACH; STRUCTURE-FUNCTION STUDIES; TRIPLE MUTANTS;

AMINO ACIDS; DICHROISM; ORGANIC ACIDS; PEPTIDES; SCAFFOLDS;

CIRCULAR DICHROISM SPECTROSCOPY;

EPITOPE; MUTANT PROTEIN; OLIGOPEPTIDE; PEPTIDE APTAMER; PROTEIN DERIVATIVE; SCAFFOLD PROTEIN; STEFIN A;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANTIGEN RECOGNITION; ARTICLE; CONTROLLED STUDY; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN VARIANT; STRUCTURE ACTIVITY RELATION;

AMINO ACID SUBSTITUTION; CIRCULAR DICHROISM; CYSTATIN A; ESCHERICHIA COLI; IMMUNOPRECIPITATION; MICROARRAY ANALYSIS; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; PROTEIN ENGINEERING; PROTEIN FOLDING; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 77951136090     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq012     Document Type: Article

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