In vitro analysis of cryopreserved alginate-poly-l-lysine-alginate-microencapsulated human hepatocytes

Liver International

Volumn 30, Issue 4, 2010, Pages 611-622

  Hang, Hualian ^a   Shi, Xiaolei ^a   Gu, Guang xiang ^b   Wu, Yafu ^a   Gu, Jinyang ^b   Ding, Yitao ^a  

^a MEDICAL SCHOOL OF NANJING UNIVERSITY   (China)

^b NANJING MEDICAL UNIVERSITY   (China)

Author keywords

Cryopreservation; Hepatocyte isolation; Hepatocytes transplantation; Human hepatocytes; Microencapsulation

Indexed keywords

2 PROPANOL; ALBUMIN; ALGINATE POLYLYSINE ALGINATE; COLLAGENASE; DIMETHYL SULFOXIDE; LIQUID NITROGEN; MESSENGER RNA;

ARTICLE; BIOARTIFICIAL LIVER; CELL CYCLE; CELL STRUCTURE; CELL THERAPY; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; HUMAN; HUMAN CELL; INCUBATION TIME; ISOLATED LIVER; LIVER CELL; MICROENCAPSULATION; PARTIAL HEPATECTOMY; PROTEIN BLOOD LEVEL; PROTEIN SECRETION; THAWING; UREA CYCLE;

ADULT; ALGINATES; ANALYSIS OF VARIANCE; BLOTTING, WESTERN; CAPSULES; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL SURVIVAL; CELL TRANSPLANTATION; CRYOPRESERVATION; FEMALE; HEPATECTOMY; HEPATOCYTES; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; POLYLYSINE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TISSUE DONORS;

EID: 77950620588     PISSN: 14783223     EISSN: 14783231     Source Type: Journal    
DOI: 10.1111/j.1478-3231.2009.02197.x     Document Type: Article

References (36)

1. Ilan Y. Towards a bank of cryopreserved hepatocytes: which cell to freeze? J Hepatol 2002, 37:145-6.
2. Caruana M, Battle T, Fuller B, Davidson B. Isolation of human hepatocytes after hepatic warm and cold ischemia: a practical approach using university of Wisconsin solution. Cryobiology 1999, 38:165-8.
3. Richert L, Alexandre E, Lloyd T. Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study. Liver Int 2004, 24:371-8.
4. Katenz E, Vondran FW, Schwartlander R. Cryopreservation of primary human hepatocytes: the benefit of trehalose as an additional cryoprotective agent. Liver Transpl 2007, 13:38-45.
5. Chen Z, Ding Y, Zhang H. Cryopreservation of suckling pig hepatocytes. Ann Clin Lab Sci 2001, 31:391-8.
6. Terry C, Dhawan A, Mitry RR, Lehec SC, Hughes RD. Preincubation of rat and human hepatocytes with cytoprotectants prior to cryopreservation can improve viability and function upon thawing. Liver Transpl 2006, 12:165-77.
7. Mahler S, Desille M, Fremond B. Hypothermic storage and cryopreservation of hepatocytes: the protective effect of alginate gel against cell damages. Cell Transplant 2003, 12:579-92.
8. Aoki T, Koizumi T, Kobayashi Y. A novel method of cryopreservation of rat and human hepatocytes by using encapsulation technique and possible use for cell transplantation. Cell Transplant 2005, 14:609-20.
9. Kusano T, Aoki T, Yasuda D. Microencapsule technique protects hepatocytes from cryoinjury. Hepatol Res 2008, 38:593-600.
10. Hang HL, Shi XL, Gu GX, Wu Y, Ding YT. Int J Artif Organs 2009, 32:720-7. A simple isolation and cryopreservation method for adult human hepatocytes
11. Vondran FW, Katenz E, Schwartlander R. Isolation of primary human hepatocytes after partial hepatectomy: criteria for identification of the most promising liver specimen. Artif Organs 2008, 32:205-13.
12. Dorko K, Freeswick PD, Bartoli F. A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. Cell Transplant 1994, 3:387-95.
13. Cai ZH, Shi ZQ, Sherman M, Sun AM. Development and evaluation of a system of microencapsulation of primary rat hepatocytes. Hepatology 1989, 10:855-60.
14. Fang S, Qiu YD, Mao L. Differentiation of embryoid-body cells derived from embryonic stem cells into hepatocytes in alginate microbeads in vitro. Acta Pharmacol Sin 2007, 28:1924-30.
15. Chen Z, Ding YT, Zhang HY. Morphology, viability and functions of suckling pig hepatocytes cultured in serum-free medium at high density. Dig Surg 2002, 19:184-91.
16. Cereghini S. Liver-enriched transcription factors and hepatocyte differentiation. FASEB J 1996, 10:267-82.
17. Terry C, Dhawan A, Mitry RR, Hughes RD. Cryopreservation of isolated human hepatocytes for transplantation: state of the art. Cryobiology 2006, 53:149-59.
18. Strain AJ, Neuberger JM. A bioartificial liver - state of the art. Science 2002, 295:1005-9.
19. Guha C, Deb NJ, Sappal BS, Ghosh SS, Roy-Chowdhury N, Roy-Chowdhury J. Amplification of engrafted hepatocytes by preparative manipulation of the host liver. Artif Organs 2001, 25:522-8.
20. Ellis AJ, Hughes RD, Wendon JA. Pilot-controlled trial of the extracorporeal liver assist device in acute liver failure. Hepatology 1996, 24:1446-51.
21. Totsugawa T, Yong C, Rivas-Carrillo JD. Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with Tamoxifen-mediated self-recombination. J Hepatol 2007, 47:74-82.
22. Wege H, Le HT, Chui MS. Telomerase reconstitution immortalizes human fetal hepatocytes without disrupting their differentiation potential. Gastroenterology 2003, 124:432-44.
23. Chen Z, Ding Y, Xu Q, Yu D. Bioartificial liver inoculated with porcine hepatocyte spheroids for treatment of canine acute liver failure model. Artif Organs 2003, 27:613-22.
24. Gu JY, Sh XL, Zhang Y, Ding YT. Heterotypic interactions in the preservation of morphology and functionality of porcine hepatocytes by bone marrow mesenchymal stem cells in vitro. J Cell Physiol 2009, 219:100-8.
25. Chu XH, Shi XL, Feng ZQ. In vitro evaluation of a multi-layer radial-flow bioreactor based on galactosylated chitosan nanofiber scaffolds. Biomaterials 2009, 30:4533-8.
26. Kulseng B, Skjak-Braek G, Ryan L. Transplantation of alginate microcapsules - generation of antibodies against alginates and encapsulated porcine islet-like cell clusters. Transplantation 1999, 67:978-84.
27. Hasse C, Zielke A, Klock G. Amitogenic alginates: key to first clinical application of microencapsulation technology. World J Surg 1998, 22:659-65.
28. Ringel M, von Mach MA, Santos R. Hepatocytes cultured in alginate microspheres: an optimized technique to study enzyme induction. Toxicology 2005, 206:153-67.
29. Thanos CG, Bintz BE, Emerich DF. Stability of alginate-polyornithine microcapsules is profoundly dependent on the site of transplantation. Journal of Biomedical Materials Research Part A 2007, 81A:1-11.
30. Pokrywczynska M, Drewa T, Jundzill A, Lysik J. Alginate is not a good material for growth of rapidly proliferating cells. Transplant Proc 2008, 40:1664-7.
31. Thanos CG, Bintz BE, Bell WJ. Intraperitoneal stability of alginate-polyornithine microcapsules in rats: an FTIR and SEM analysis. Biomaterials 2006, 27:3570-79.
32. Murua A, Orive G, Hernandez RM, Pedraz JL. Cryopreservation based on freezing protocols for the long-term storage of microencapsulated myoblasts. Biomaterials 2009, 30:3495-501.
33. Alexandre E, Viollon-Abadie C, David P. Cryopreservation of adult human hepatocytes obtained from resected liver biopsies. Cryobiology 2002, 44:103-13.
34. Dou M, Desousa G, Lacarelle B. Thawed human hepatocytes in primary culture. Cryobiology 1992, 29:454-69.
35. Rialland L, Guyomard C, Scotte M, Chesne C, Guillouzo A. Viability and drug metabolism capacity of alginate-entrapped hepatocytes after cryopreservation. Cell Biol Toxicol 2000, 16:105-16.
36. Koizumi T, Aoki T, Kobayashi Y. Long-term maintenance of the drug transport activity in cryopreservation of microencapsulated rat hepatocytes. Cell Transplant 2007, 16:67-73.