Optimised mammalian expression through the coupling of codon adaptation with gene amplification: Maximum yields with minimum effort

Journal of Biotechnology

Volumn 146, Issue 4, 2010, Pages 186-193

  Kotsopoulou, Ekaterini ^a   Bosteels, Hella ^a   Chim, Yuen Ting ^a   Pegman, Pamela ^a   Stephen, George ^a   Thornhill, Susannah I ^a   Faulkner, James D ^a   Uden, Mark ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Antibody expression; CAI; CHO; Codon adaptation; Gene amplification; Methotrexate

Indexed keywords

ANTIBODY EXPRESSION; DIHYDROFOLATE REDUCTASE; GENE AMPLIFICATION; HIGH YIELD; HIGH-PRODUCING CLONES; HOST CELLS; MAMMALIAN CELLS; OPTIMISATIONS; RECOMBINANT PROTEIN;

AMPLIFICATION; ANTIBODIES; GENETIC ENGINEERING; MAMMALS;

GENE EXPRESSION;

DIHYDROFOLATE REDUCTASE; METHOTREXATE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CHO CELL; CODON ADAPTATION; CONTROLLED STUDY; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC ENGINEERING; GENOMICS; HOST CELL; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION;

ANIMALS; CHO CELLS; CODON; CRICETINAE; CRICETULUS; GENE AMPLIFICATION; GENE DOSAGE; METHOTREXATE; OPEN READING FRAMES; RECOMBINANT PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE;

MAMMALIA;

EID: 77950520198     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.02.004     Document Type: Article

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