A "Humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells

Journal of Virology

Volumn 70, Issue 7, 1996, Pages 4646-4654

  Zolotukhin, Sergei ^a   Potter, Mark ^a   Hauswirth, William W ^a   Guy, John ^a   Muzyczka, Nicholas ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; VIRUS PROTEIN; VIRUS VECTOR;

ADENOVIRUS; ANIMAL CELL; ARTICLE; FLUORESCENCE ACTIVATED CELL SORTER; FLUORESCENCE ANALYSIS; GENE EXPRESSION REGULATION; JELLYFISH; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE, TRANSFORMED; CLONING, MOLECULAR; DEPENDOVIRUS; DNA, COMPLEMENTARY; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; GUINEA PIGS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; RETINA; SCYPHOZOA;

EID: 0029995531     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.7.4646-4654.1996     Document Type: Article

References (36)

1. Beniwll, J., J. Wilson, D. Sun, B. Forbes, and A. Maguire. 1994. Adenovirus vector-mediated in vivo gene transfer into adult murine retina. Invest. Ophthalmol. Visual Sci. 35:2535-2542.
2. Bennetzen, J. L., and B. D. Hall. 1982. Codon selection in yeast. J. Biol. Chem. 257:3026-3031.
3. Bell, A. J., W. Haddara, L. Prevec, and F. L. Graham. 1994. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc. Natl. Acad. Sci. USA 91:8802-8806.
4. Chalnfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
5. Cheung, A. K., M. D. Hoggan, W. W. Hauswirth, and K. I. Berns. 1980. Integration of the adeno-associated virus genome into cellular DNA in latently infected human Detroit 6 cells. J. Virol. 33:739-748.
6. Cody, C. W., D. C. Prasher, W. M. Westler, F. G. Prendergast, and W. W. Ward. 1993. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry 32:1212-1218.
7. Dirks, W., M. Wirth, and H. Hauser. 1993. Dicistronic transcription units for gene expression in mammalian cells. Gene 128:247-249.
8. Grantham, R., C. Gautier, M. Gouy, M. Jacobzone, and R. Mercier. 1981. Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 9:r43-r74.
9. Heim, R., A. B. Cubitt, and R. Y. Tsien. 1995. Improved green fluorescence. Nature (London) 373:663-664. (Letter.)
10. Heim, R., D. C. Prasher, and R. Y. Tsien. 1994. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91:12501-12504.
11. Ikemura, T. 1980. The frequency of codon usage in E. coli genes: correlation with abundance of cognate tRNA, p. 519-534. In S. Osawa et al. (ed.). Genetics and evolution of RNA polymerase, tRNA, and ribosomes. University of Tokyo Press, Tokyo, and Elsevier/North Holland, Amsterdam.
12. Ikemura, T. 1981. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes. J. Mol. Biol. 146:1-21.
13. Ikemura, T. 1981. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system. J. Mol. Biol. 151:389-409.
14. Ikemura, T. 1982. Correlation between the abundance of yeast transfer RNAs and the occurrence of the respective codons in protein genes. Differences in synonymous codon choice patterns of yeast and Escherichia coli with reference to the abundance of isoaccepting transfer RNAs. J. Mol. Biol. 158:573-597.
15. Jackson, R. J., M. T. Howell, and A. Kaminski. 1990. The novel mechanism of initiation of picornavirus RNA translation. Trends Biochem. Sci. 15:477-483.
16. Jang, S. K., H. G. Krausslich, M. J. Nicklin, G. M. Duke, A. C. Palmenberg, and E. Wimmer. 1988. A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J. Virol. 62:2636-2643.
17. Kotin, R. M. 1996. Prospects for the use of adeno-associated virus as a vector for human gene therapy. Hum. Gene Ther. 5:793-801.
18. Kozak, M. 1987. At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. J. Mol. Biol. 196:947-950.
19. Kozak, M. 1990. Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes. Proc. Natl. Acad. Sci. USA 87: 8301-8305.
20. Laughlin, C. A., C. B. Cardellichio, and H. C. Coon. 1986. Latent infection of KB cells with adeno-associated virus type 2. J. Virol. 60:515-524.
21. Levy, J. P., R. R. Muldoon, S. Zolotukhin, and C. J. Link, Jr. 1996. Retroviral transfer and expression of a humanized, red shifted green fluorescent protein gene into human tumor cells. Nat. Biotechnol. 14:610-614.
22. Li, T., M. Adamian, D. J. Roof, E. L. Berson, T. P. Dryja, B. J. Roessler, and B. L. Davidson. 1994. In vivo transfer of a reporter gene to the retina mediated by an adenoviral vector. Invest. Ophthalmol. Visual Sci. 35:2543-2549.
23. Macejak, D. G., and P. Sarnow. 1991. Internal initiation of translation mediated by the 5′ leader of a cellular mRNA. Nature (London) 353:90-94.
24. McCarry, D. M., M. Christensen, and N. Muzyczka. 1991. Sequences required for coordinate induction of adeno-associated virus p19 and p40 promoters by Rep protein. J. Virol. 65:2936-2945.
25. McGrory, W. J., D. S. Bautista, and F. L. Graham. 1988. A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5. Virology 163:614-617.
26. McLaughlin, S. K., P. Collis, P. L. Hermonat, and N. Muzyczka. 1988. Adeno-associated virus general transduction vectors: analysis of proviral structures. J. Virol. 62:1963-1973.
27. Morin, J. G., and J. W. Hastings. 1971. Energy transfer in a bioluminescent system. J. Cell Physiol. 77:313-318.
28. Muzyczka, N. 1992. Use of adeno-associated virus as a general transduction vector for mammalian cells. Curr. Top. Microbiol. Immunol. 158:97-129.
29. Prasher, D. C., V. K. Eckenrode, W. W. Ward, F. G. Prendergast, and M. J. Cormier. 1992. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229-233.
30. Ryan, J. H., S. Zolotukhin, and N. Muzyczka. 1996. Sequence requirements for binding of Rep68 to the adeno-associated virus terminal repeats. J. Virol. 79:1542-1553.
31. Samulski, R. J., L. S. Chang, and T. Shenk. 1989. Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression. J. Virol. 83:3822-3828.
32. Shimomura, O. 1979. Structure of the chromophore of the Aequorea green fluorescent protein. FEBS Lett. 104:220-222.
33. Snyder, R. O., D. S. Im, T. Ni, X. Xiao, R. J. Samulski, and N. Muzyczka. 1993. Features of the adeno-associated virus origin involved in substrate recognition by the viral Rep protein. J. Virol. 67:6096-6104.
34. Wada, K., S. Aota, R. Tsuchiya, F. Ishibashi, T. Gojobori, and T. Ikemura. 1990. Codon usage tabulated from the GenBank genetic sequence data. Nucleic Acids Res. 18(Suppl.):2367-2411.
35. Ward, P., E. Urceiay, R. Kotin, B. Safer, and K. I. Berns. 1994. Adenoassociated virus DNA replication in vitro: activation by a maltose-binding protein/Rep 68 fusion protein. J. Virol. 68:6029-6037.
36. Ward, W. W., C. W. Cody, R. C. Hart, and M. J. Cormier. 1980. Spectrophotomelric identity of the energy-transfer chromophores in Renilla and Aequorea green fluorescent proteins. Photochem. Photobiol. 31:611-615.