Kinetics and substrate selectivity of a Triticum aestivum xylanase inhibitor (TAXI) resistant D11F/R122D variant of Bacillus subtilis XynA xylanase

Journal of Biotechnology

Volumn 146, Issue 4, 2010, Pages 207-214

  Rasmussen, Louise E ^a   Sørensen, Jens F ^b   Meyer, Anne S ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b DANISCO A S   (Denmark)

Author keywords

Arabinoxylan; Endoxylanase; Inhibition; Selectivity; TAXI; Xylo oligosaccharide profile

Indexed keywords

ARABINOXYLANS; BACILLUS SUBTILIS; CATALYTIC RATES; ENDOXYLANASE; EQUIMOLAR ADDITION; HIGHER-DEGREE; INITIAL RATE; MOLAR RATIO; PRODUCT YIELDS; PROLONGED REACTION; STRUCTURAL BASIS; STRUCTURAL POINT; SUBSTRATE SELECTIVITY; THREE-DIMENSIONAL STRUCTURE; TRITICUM AESTIVUM; WATER-EXTRACTABLE ARABINOXYLAN; WHEAT BRAN; XYLANASE INHIBITOR; XYLANASES; XYLO- OLIGOSACCHARIDES;

BACTERIOLOGY; OLIGOSACCHARIDES; SULFUR COMPOUNDS;

SUBSTRATES;

ARABINOXYLAN; BACTERIAL PROTEIN; HYDROLASE INHIBITOR; OLIGOSACCHARIDE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLANASE INHIBITOR; XYNA XYLANASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; BACTERIUM MUTANT; BIOENGINEERING; CATALYSIS; CELL ACTIVITY; CONTROLLED STUDY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; NONHUMAN; POINT MUTATION; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; STRAIN DIFFERENCE; WHEAT; WHEAT BRAN;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CHROMATOGRAPHY, ION EXCHANGE; DIETARY FIBER; ENDO-1,4-BETA XYLANASES; MODELS, MOLECULAR; PLANT PROTEINS; SUBSTRATE SPECIFICITY; XYLANS;

BACILLUS SUBTILIS; TRITICUM AESTIVUM;

EID: 77950459770     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.02.012     Document Type: Article

References (38)

1. Arnous A., Meyer A.S. Comparison of methods for compositional characterization of grape (Vitis vinifera L.) and apple (Malus domestica) skins. Food Bioprod. Process. 2008, 86:79-86.
2. Bedford M.R. Factors influencing the use of enzymes in cereal-based diets. Recent Advances in Enzymes in Grain Processing. Laboratory of Food Chemistry 2003, 371-379. K.U. Leuven, Leuven, Belgium. C.M. Courtin, W.S. Veraverbeke, J.A. Delcour (Eds.).
3. Berrin J., Ajandouz E.H., Georis J., Arnaut F., Juge N. Substrate and product hydrolysis specificity in family 11 glycosidases: an analysis of Penicillium funiculosum and Penicillium griseofulvum xylanases. Appl. Microbiol. Biotechnol. 2007, 74:1001-1010.
4. Biely P. Microbial xylanolytic systems. Trends Biotechnol. 1985, 3:286-290.
5. Biely P., Vrsanská M., Tenkanen M., Kluepfel D. Endo-β-1,4-xylanase families: differences in catalytic properties. J. Biotechnol. 1997, 57:151-166.
6. Bonnin E., Daviet S., Gebruers K., Delcour J.A., Goldson A., Juge N. Variation in the levels of the different xylanase inhibitors in grain and flour of 20 French wheat cultivars. J. Cereal Sci. 2005, 41:375-379.
7. Bonnin E., Daviet S., Sorensen J.F., Sibbesen O., Goldson A., Juge N., Saulnier L. Behaviour of family 10 and 11 xylanases towards arabinoxylans with varying structure. J. Sci. Food Agric. 2006, 86:1618-1622.
8. Bray M.R., Clarke A.J. Action pattern of xylo-oligosaccharides hydrolysis by Schizophyllum commune xylanase A. Eur. J. Biochem. 1992, 204:191-196.
9. Campbell G.L., Bedford M.R. Enzyme applications for monogastric feeds: a review. Can. J. Anim. Sci. 1992, 72:449-466.
10. Cantarel B.L., Coutinho P.M., Rancurel C., Bernard T., Lombard V., Henrissat B. The carbohydrate-active enzymes database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res. 2009, 37:D233-D238.
11. Collins T., Gerday C., Feller G. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol. Rev. 2005, 29:3-23.
12. Courtin C.M., Delcour J.A. Arabinoxylans and endoxylanases in wheat flour breadmaking. J. Cereal Sci. 2002, 35:225-243.
13. Courtin C.M., Gys W., Gebruers K., Delcour J.A. Evidence for the involvement of arabinoxylan and xylanases in refrigerated dough syruping. J. Agric. Food Chem. 2005, 53:7623-7629.
14. Croes E., Gebruers K., Robben J., Noben J., Samyn B., Debyser G., Beeumen J.V., Delcour J.A., Courtin C.M. Variability of polymorphic families of three types of xylanase inhibitors in the wheat grain proteome. Proteomics 2008, 8:1692-1705.
15. Debyser W., Derdelinckx G., Delcour J.A. Arabinoxylan solubilization and inhibition of the barley malt xylanolytic system by wheat during mashing with wheat wholemeal adjunct: evidence for a new class of enzyme inhibitors in wheat. J. Am. Soc. Brew. Chem. 1997, 55:153-156.
16. Ferreira-Filho E.X.F. The xylan-degrading enzyme system. Braz. J. Med. Biol. Res. 1994, 27:1093-1109.
17. Fierens K., Gils A., Sansen S., Brijs K., Courtin C.M., Declerck P.J., De Ranter C.J., Gebruers K., Rabijns A., Robben J., Van Campenhout S., Volckaert G., Delcour J.A. His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolyse family 11 endoxylanase. FEBS J. 2005, 272:5872-5882.
18. Fierens E., Gebruers K., Goesaert H., Brijs K., Beaugrand J., Van Campenhout S., Courtin C.M., Delcour J.A., Rombouts S., Volckaert G., Proost P. TLXI, a novel type of xylanase inhibitor from wheat (Triticum aestivum) belonging to the thaumatin family. Biochem. J. 2007, 403:583-591.
19. Fierens E., Gebruers K., Courtin C.M., Delcour J.A. Xylanase inhibitors bind to nonstarch polysaccharides. J. Agric. Food Chem. 2008, 56:564-570.
20. Frederix S.A., Courtin C.M., Delcour J.A. Substrate selectivity and inhibitor sensitivity affect xylanase functionality in wheat flour gluten-starch separation. J. Cereal Sci. 2004, 40:41-49.
21. Gebruers K., Debyser W., Goesaert H., Proost P., Van Damme J., Delcour J.A. Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificities. J. Biochem. 2001, 353:239-244.
22. Gebruers K., Courtin C.M., Moers K., Noots I., Trogh I., Delcour J.A. The breadmaking potential of two Aspergillus niger xylanases is strongly dictated by their substrate selectivities and inhibitor sensitivities. Enzyme Microb. Technol. 2005, 417-425.
23. Katapodis P., Kavarnou A., Kintzios S., Pistola E., Kekos D., Macris B.J., Christakopoulos P. Production of acidic xylo-oligosaccharides by a family 10 endoxylanase from Thermoascus aurantiacus and use as plant growth regulators. Biotechnol. Lett. 2002, 24:1413-1416.
24. McLauchlan W.R., Garcia-Conesa M.T., Williamson G., Roza M. A novel class of protein from wheat which inhibits xylanases. Biochem. J. 1999, 338:441-446.
25. Meagher M.M., Tao B.Y., Chow J.M., Reilly P. Kinetics and subsite mapping of a d-xylobiose and d-xylose-producing Aspergillus niger endo-(1,4)-β-d-xylanase. Carbohydr. Res. 1988, 173:273-283.
26. Moers K., Courtin C.M., Brijs K., Delcour J.A. A screening method for endo-β-1,4-xylanase substrate selectivity. Anal. Biochem. 2003, 319:73-77.
27. Moers K., Celus I., Brijs K., Courtin C.M., Delcour J.A. Endoxylanase substrate selectivity determines degradation of wheat water-extractable and water-unextractable arabinoxylan. Carbohydr. Res. 2005, 340:1319-1327.
28. Pastell H., Westermann P., Meyer A.S., Tuomainen P., Tenkanen M. In vitro fermentation of arabinoxylan-derived carbohydrates by Bifidobacteria and mixed fecal microbiota. J. Agric. Food Chem. 2009, 57:8598-8606.
29. Pollet A., Vandermarliere E., Lammertyn J., Strelkov S.V., Delcour J.A., Courtin C.M. Crystallographic and activity-based evidence for thumb flexibility and its relevance in glycoside hydrolase family 11 xylanases. Proteins 2009, 77:395-403.
30. Rouau X., Surget A. Evidence for the presence of a pentosanase inhibitor in wheat flours. J. Cereal Sci. 1998, 28:63-70.
31. Sansen S., De Ranter C.J., Gebruers K., Brijs K., Courtin C.M., Delcour J.A., Rabijns A. Structural basis for inhibition of Aspergillus niger xylanase by Triticum aestivum xylanase inhibitor-I. J. Biol. Chem. 2004, 279:36022-36028.
32. Sørensen J.F., Sibbesen O. Mapping of residues involved in the interaction between the Bacillus subtilis xylanase A and proteinaceous wheat xylanase inhibitors. Protein Eng. Des. Sel. 2006, 19:205-210.
33. Sørensen H.R., Meyer A.S., Pedersen S. Enzymatic hydrolysis of water-soluble wheat arabinoxylan. 1. Synergy between α-l-arabinofuranosidases, endo-1,4-β-xylanases, and β-xylosidase activity. Biotechnol. Bioeng. 2003, 81:726-731.
34. Sørensen J.F., Kragh K.M., Sibbesen O., Delcour J., Goesaert H., Svensson B., Tahir T.A., Brufau J., Perez-Vendrell A.M., Bellincampi D., D'Ovidio R., Camardella L., Giovane A., Bonnin E., Juge N. Potential role of glycosidase inhibitors in industrial biotechnological applications. Biochem. Biophys. Acta 2004, 1696:275-287.
35. Sørensen H.R., Pedersen S., Meyer A.S. Optimization of reaction conditions for enzymatic viscosity reduction and hydrolysis of wheat arabinoxylan in an industrial ethanol fermentation residue. Biotechnol. Prog. 2006, 22:505-513.
36. Sørensen H.R., Pedersen S., Jørgensen C.T., Meyer A.S. Enzymatic hydrolysis of wheat arabinoxylan by a recombinant " minimal" enzyme cocktail containing β-xylosidase and novel endo-1,4-β-xylanase and α-l-arabinofuranosidase activities. Biotechnol. Prog. 2007, 23:100-107.
37. Trogh I., Sørensen J.F., Courtin C.M., Delcour J.A. Impact of inhibition sensitivity on endoxylanase functionality in wheat flour breadmaking. J. Agric. Food Chem. 2004, 52:4296-4302.
38. Van der Borght A., Goesaert H., Veraverbeke W.S., Delcour J.A. Fractionation of wheat and wheat flour into starch and gluten: overview of the main processes and the factors involved. J. Cereal Sci. 2005, 41:221-237.