Design and characterization of a constitutively open KcsA

FEBS Letters

Volumn 584, Issue 6, 2010, Pages 1133-1138

  Cuello, Luis G ^a   Jogini, Vishwanath ^a   Cortes, D Marien ^a   Sompornpisut, Amornrat ^a   Purdy, Michael D ^c   Wiener, Michael C ^c   Perozo, Eduardo ^a,b  

^a UNIVERSITY OF CHICAGO   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

^c UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Activation gate; Crystal structure; K+ Channel; KcsA; PH sensor

Indexed keywords

MUTANT PROTEIN; POTASSIUM CHANNEL; POTASSIUM CHANNEL KCSA; PROTON; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN STRUCTURE; SPECTROSCOPY; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ION CHANNEL GATING; MODELS, MOLECULAR; MUTANT PROTEINS; POTASSIUM CHANNELS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STRUCTURAL HOMOLOGY, PROTEIN;

EID: 77950367690     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2010.02.015     Document Type: Article

References (38)

1. + conduction and selectivity. Science 1998, 280:69-77.
2. + channel can be trapped in the open state by an inter subunit metal bridge. Neuron 1998, 21:617-621.
3. + channel. Neuron 1997, 19:175-184.
4. + channel studied by EPR spectroscopy. Nat. Struct. Biol. 1998, 5:459-469.
5. +-channel activation gating. Science 1999, 285:73-78.
6. + channels defined by high-affinity metal bridges. Nature 2004, 428:864-868.
7. Yellen G. The moving parts of voltage-gated ion channels. Q. Rev. Biophys. 1998, 31:239-295.
8. + channel and its structural implications. Nature 2000, 403:321-325.
9. + channel during gating. Neuron 1996, 16:859-867.
10. Chapman M.L., VanDongen H.M., VanDongen A.M. Activation-dependent subconductance levels in the drk1 K channel suggest a subunit basis for ion permeation and gating. Biophys. J. 1997, 72:708-719.
11. Chapman M.L., VanDongen A.M. K channel subconductance levels result from heteromeric pore conformations. J. Gen. Physiol. 2005, 126:87-103.
12. Gao L., Mi X., Paajanen V., Wang K., Fan Z. Activation-coupled inactivation in the bacterial potassium channel KcsA. Proc. Natl. Acad. Sci. USA 2005, 102:17630-17635.
13. Blunck R., Cordero-Morales J.F., Cuello L.G., Perozo E., Bezanilla F. Detection of the opening of the bundle crossing in KcsA with fluorescence lifetime spectroscopy reveals the existence of two gates for ion conduction. J. Gen. Physiol. 2006, 128:569-581.
14. Cordero-Morales J.F., Cuello L.G., Zhao Y., Jogini V., Cortes D.M., Roux B., Perozo E. Molecular determinants of gating at the potassium-channel selectivity filter. Nat. Struct. Mol. Biol. 2006, 13:311-318.
15. Chakrapani S., Cordero-Morales J.F., Perozo E. A quantitative description of KcsA gating II: single-channel currents. J. Gen. Physiol. 2007, 130:479-496.
16. Chakrapani S., Cordero-Morales J.F., Perozo E. A quantitative description of KcsA gating I: macroscopic currents. J. Gen. Physiol. 2007, 130:465-478.
17. Cordero-Morales J.F., Jogini V., Lewis A., Vasquez V., Cortes D.M., Roux B., Perozo E. Molecular driving forces determining potassium channel slow inactivation. Nat. Struct. Mol. Biol. 2007, 14:1062-1069.
18. Liu Y.S., Sompornpisut P., Perozo E. Structure of the KcsA channel intracellular gate in the open state. Nat. Struct. Biol. 2001, 8:883-887.
19. Jiang Y., Lee A., Chen J., Cadene M., Chait B.T., MacKinnon R. The open pore conformation of potassium channels. Nature 2002, 417:523-526.
20. Jiang Y., Lee A., Chen J., Cadene M., Chait B.T., MacKinnon R. Crystal structure and mechanism of a calcium-gated potassium channel. Nature 2002, 417:515-522.
21. Kelly B.L., Gross A. Potassium channel gating observed with site-directed mass tagging. Nat. Struct. Biol. 2003, 10:280-284.
22. Bezanilla F. How membrane proteins sense voltage. Nat. Rev. Mol. Cell Biol. 2008, 9:323-332.
23. Swartz K.J. Sensing voltage across lipid membranes. Nature 2008, 456:891-897.
24. Changeux J.P., Edelstein S.J. Allosteric mechanisms of signal transduction. Science 2005, 308:1424-1428.
25. Sine S.M., Engel A.G. Recent advances in Cys-loop receptor structure and function. Nature 2006, 440:448-455.
26. Cuello L.G., Cortes D.M., Jogini V., Somporpisut A., Perozo E. A molecular mechanism for proton-dependent gating in KcsA. FEBS Lett. 2010, 584:1126-1132.
27. Thompson A.N., Posson D.J., Parsa P.V., Nimigean C.M. Molecular mechanism of pH sensing in KcsA potassium channels. Proc. Natl. Acad. Sci. USA 2008, 105:6900-6905.
28. + channel (SKC1): oligomeric stoichiometry and stability. Biochemistry 1997, 36:10343-10352.
29. + channel. Biochemistry 1998, 37:3229-3236.
30. Baker N.A., Sept D., Joseph S., Holst M.J., McCammon J.A. Electrostatics of nanosystems: application to microtubules and the ribosome. Proc. Natl. Acad. Sci. USA 2001, 98:10037-10041.
31. Humphrey W., Dalke A., Schulten K. VMD: visual molecular dynamics. J. Mol. Graph. 1996, 14(33-8):27-28.
32. + channel-Fab complex at 2.0 A resolution. Nature 2001, 414:43-48.
33. Otwinowski Z.M.W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 1997, 276:307-332.
34. Brunger A.T., et al. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr., D Biol. Crystallogr. 1998, 54:905-921.
35. Jones T.A., Zou J.Y., Cowan S.W., Kjeldgaard M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr., A 1991, 47(Pt 2):110-119.
36. Paynter J.J., Sarkies P., Andres-Enguix I., Tucker S.J. Genetic selection of activatory mutations in KcsA. Channels (Austin) 2008, 2:413-418.
37. +-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch. Microbiol. 1997, 167:126-136.
38. Akabas M.H., Stauffer D.A., Xu M., Karlin A. Acetylcholine receptor channel structure probed in cysteine-substitution mutants. Science 1992, 258:307-310.