Genetic selection of activatory mutations in KcsA

Channels

Volumn 2, Issue 6, 2008, Pages 413-418

  Paynter, Jennifer J ^a   Sarkies, Peter ^a   Andres Enguix, Isabelle ^a   Tucker, Stephen J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Genetic complementation; Ion channel gating; K+ auxotrophic; K+ channel; KcsA; Potassium channel

Indexed keywords

POTASSIUM CHANNEL; BACTERIAL PROTEIN; ESCHERICHIA COLI PROTEIN; KCSA PROTEIN, E COLI; PROKARYOTIC POTASSIUM CHANNEL;

ARTICLE; CELL PH; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE MUTATION; GENETIC COMPLEMENTATION; GENETIC SELECTION; NONHUMAN; PH; PHENOTYPE; SACCHAROMYCES CEREVISIAE; STREPTOMYCES LIVIDANS; GENETICS; MUTATION; PHYSIOLOGY;

PROKARYOTA; STREPTOMYCES LIVIDANS;

BACTERIAL PROTEINS; ESCHERICHIA COLI PROTEINS; GENETIC COMPLEMENTATION TEST; HYDROGEN-ION CONCENTRATION; MUTATION; POTASSIUM CHANNELS; STREPTOMYCES LIVIDANS;

EID: 64149115115     PISSN: 19336950     EISSN: 19336969     Source Type: Journal    
DOI: 10.4161/chan.2.6.6874     Document Type: Article

References (35)

1. Cortes DM, Cuello LG, Perozo E. Molecular architecture of full-length KcsA: role of cytoplasmic domains in ion permeation and activation gating. J Gen Physiol 2001; 117:165-180 (Pubitemid 32423754)
2. +-channel activation gating. Science 1999; 285:73-78
3. Cordero-Morales JF, Cuello LG, Zhao Y, Jogini V, Cortes DM, Roux B, Perozo E. Molecular determinants of gating at the potassium-channel selectivity filter. Nat Struct Mol Biol 2006; 13:311-318 (Pubitemid 43873501)
4. + channel. Nat Struct Mol Biol 2008; 15:605-612
5. Zakharian E, Reusch RN. Streptomyces lividans potassium channel KcsA is regulated by the potassium electrochemical gradient. Biochem Biophys Res Commun 2004; 316:429-436
6. Negoda A, Xian M, Reusch RN. Insight into the selectivity and gating functions of Streptomyces lividans KcsA. Proc Natl Acad Sci USA 2007; 104:4342-4346
7. Corvini PF, Gautier H, Rondags E, Vivier H, Goergen JL, Germain P. Intracellular pH determination of pristinamycin-producing Streptomyces pristinaespiralis by image analysis. Microbiology 2000; 146:2671-2678
8. Hegermann J, Overbeck J, Schrempf H. In vivo monitoring of the potassium channel KcsA in Streptomyces lividans hyphae using immuno-electron microscopy and energy-filtering transmission electron microscopy. Microbiology 2006; 152:2831-2841
9. + uptake-deficient Escherichia coli. Methods Mol Biol 2006; 337:157-165
10. Parfenova LV, Crane BM, Rothberg BS. Modulation of MthK potassium channel activity at the intracellular entrance to the pore. J Biol Chem 2006; 281:21131-21138
11. + channel: tryptophan scanning and complementation analysis lead to mutants with altered gating. Biochemistry 2002; 41:13653-13662
12. Epstein W. The roles and regulation of potassium in bacteria. Prog Nucleic Acid Res Mol Biol 2003; 75:293-320.
13. Sun S, Gan JH, Paynter JJ, Tucker SJ. Cloning and functional characterization of a superfamily of microbial inwardly rectifying potassium channels. Physiol Genomics 2006; 26:1-7. (Pubitemid 43974217)
14. Yi BA, Lin YF, Jan YN, Jan LY. Yeast screen for constitutively active mutant G proteinactivated potassium channels. Neuron 2001; 29:657-667
15. Bichet D, Lin YF, Ibarra CA, Huang CS, Yi BA, Jan YN, Jan LY. Evolving potassium channels by means of yeast selection reveals structural elements important for selectivity. Proc Natl Acad Sci USA 2004; 101:4441-4446 (Pubitemid 38437430)
16. Chatelain FC, Alagem N, Xu Q, Pancaroglu R, Reuveny E, Minor DL Jr. The pore helix dipole has a minor role in inward rectifier channel function. Neuron 2005; 47:833-843 (Pubitemid 41266950)
17. Sesti F, Rajan S, Gonzalez-Colaso R, Nikolaeva N, Goldstein SA. Hyperpolarization moves S4 sensors inward to open MVP, a methanococcal voltage-gated potassium channel. Nat Neurosci 2003; 6:353-361 (Pubitemid 36373980)
18. Shechter E, Letellier L, Simons ER. Fluorescence dye as monitor of internal pH in Escherichia coli cells. FEBS Lett 1982; 139:121-124
19. Imai T, Ohno T. Measurement of yeast intracellular pH by image processing and the change it undergoes during growth phase. J Biotechnol 1995; 38:165-172
20. + channel-Fab complex at 2.0 A resolution. Nature 2001; 414:43-48
21. Sansom MS, Bond PJ, Deol SS, Grottesi A, Haider S, Sands ZA. Molecular simulations and lipid-protein interactions: potassium channels and other membrane proteins. Biochem Soc Trans 2005; 33:916-920 (Pubitemid 41659078)
22. Kariev AM, Znamenskiy VS, Green ME. Quantum mechanical calculations of charge effects on gating the KcsA channel. Biochim Biophys Acta 2007.
23. + channel by lateral forces applied to the C-termini of inner helices. Biophys J 2004; 87:1526-1536
24. Takeuchi K, Takahashi H, Kawano S, Shimada I. Identification and characterization of the slowly exchanging pH-dependent conformational rearrangement in KcsA. J Biol Chem 2007; 282:15179-15186 (Pubitemid 47093340)
25. Thompson AN, Posson DJ, Parsa PV, Nimigean CM. Molecular mechanism of pH sensing in KcsA potassium channels. Proc Natl Acad Sci USA 2008; 105:6900-6905
26. Rapedius M, Paynter JJ, Fowler PW, Shang L, Sansom MS, Tucker SJ, Baukrowitz T. Control of pH and PIP2 gating in heteromeric Kir4.1/Kir5.1 channels by H-bonding at the helix-bundle crossing. Channels 2007; 1:327-330
27. Rapedius M, Haider S, Browne KF, Shang L, Sansom MS, Baukrowitz T, Tucker SJ. Structural and functional analysis of the putative pH sensor in the Kir1.1 (ROMK) potassium channel. EMBO Rep 2006; 7:611-616 (Pubitemid 43827328)
28. Rapedius M, Fowler PW, Shang L, Sansom MS, Tucker SJ, Baukrowitz T. H bonding at the helix-bundle crossing controls gating in Kir potassium channels. Neuron 2007; 55:602-614 (Pubitemid 47223642)
29. Cordero-Morales JF, Cuello LG, Perozo E. Voltage-dependent gating at the KcsA selectivity filter. Nat Struct Mol Biol 2006; 13:319-322 (Pubitemid 43873502)
30. Gao L, Mi X, Paajanen V, Wang K, Fan Z. Activation-coupled inactivation in the bacterial potassium channel KcsA. Proc Natl Acad Sci USA 2005; 102:17630-17635 (Pubitemid 41803548)
31. + channel inhibitor identified by high-throughput screening in yeast. Mol Pharmacol 2004; 65:214-219
32. Nikawa J, Kodaki T, Yamashita S. Expression of the Saccharomyces cerevisiae PIS gene and synthesis of phosphatidylinositol in Escherichia coli. J Bacteriol 1988; 170:4727-4731
33. Nimigean CM, Chappie JS, Miller C. Electrostatic tuning of ion conductance in potassium channels. Biochemistry 2003; 42:9263-9268 (Pubitemid 36959233)
34. Minor DL Jr, Masseling SJ, Jan YN, Jan LY. Transmembrane structure of an inwardly rectifying potassium channel. Cell 1999; 96:879-891 (Pubitemid 29165090)
35. Hamilton R, Watanabe CK, de Boer HA. Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs. Nucleic Acids Res 1987; 15:3581-3593