Blocker protection in the pore of a voltage-gated K+ channel and its structural implications

Nature

Volumn 403, Issue 6767, 2000, Pages 321-325

  Del Camino, Donate ^a   Holmgren, Miguel ^a   Liu, Y ^a   Yellen, Gary ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POTASSIUM CHANNEL; VOLTAGE GATED CHANNEL FORMING PROTEIN;

ARTICLE; CHANNEL GATING; CHEMICAL MODIFICATION; ELECTRICITY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN STRUCTURE;

BACTERIAL PROTEINS; CYSTEINE; ELECTROSTATICS; HYDROGEN BONDING; ION CHANNEL GATING; MESYLATES; MODELS, MOLECULAR; PEPTIDES; POTASSIUM; POTASSIUM CHANNEL BLOCKERS; POTASSIUM CHANNELS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; QUATERNARY AMMONIUM COMPOUNDS; SHAKER SUPERFAMILY OF POTASSIUM CHANNELS; TETRAETHYLAMMONIUM;

BACTERIA (MICROORGANISMS);

EID: 0034688285     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35002099     Document Type: Article

References (27)

1. Doyle, D. A. et al. The structure of the potassium channel: molecular basis of potassium conduction and selectivity. Science 280, 69-77 (1998).
2. Armstrong, C. M. Interaction of tetraethylammonium ion derivatives with the potassium channels of giant axon. J. Gen. Physiol. 58, 413-437 (1971).
3. + channel. Neuron 19, 175-184 (1997).
4. Yellen, G. The moving parts of voltage-gated ion channels. Q. Rev. Biophys. 31, 239-296 (1998).
5. + channels by a peptide derived from ShB. Science 250, 568-571 (1990).
6. Choi, K. L., Mossman, C., Aubé, J. & Yellen, G. The internal quaternary ammonium receptor site of Shaker potassium channels. Neuron 10, 533-541 (1993).
7. + channels. Proc. Natl Acad. Sci. USA 93, 13357-13361 (1996).
8. + channel examined through cysteine mutagenesis and chemical modification. J. Gen. Physiol. 108, 195-206 (1996).
9. + channels. Proc. Natl Acad. Sci. USA 88, 5092-5095 (1991).
10. + channel behaves like an open-channel blocker. Neuron 7, 743-753 (1991).
11. MacArthur, M. W. & Thornton, J. M. Influence of proline residues on protein conformation. J. Mol. Biol. 218, 397-412 (1991).
12. Durell, S. R. & Guy, H. R. Atomic scale structure and functional models of voltage-gated potassium channels. Biophys. J. 62, 238-247 (1992).
13. + channel can be trapped in the open state by an intersubunit metal bridge. Neuron 21, 617-621 (1998).
14. Gross, A. & MacKinnon, R. Agitoxin footprinting the Shaker potassium channel pore. Neuron 16, 399-406 (1996).
15. Lee, M. S., Gippert, G. P., Soman, K. V., Case, D. A. & Wright, P. E. Three-dimensional solution structure of a single zinc finger DNA-binding domain. Science 245, 635-637 (1989).
16. +-channel activation gating. Science 285, 73-78 (1999).
17. Kamb, A., Tseng-Crank, J. C. L. & Tanouye, M. A. Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity. Neuron 1, 421-430 (1988).
18. Hoshi, T., Zagotta, W. N. & Aldrich, R. W. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science 250, 533-538 (1990).
19. López-Barneo, J., Hoshi, T., Heinemann, S. H. & Aldrich, R. W. Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels. Receptors Channels 1, 61-71 (1993).
20. Jurman, M. E., Boland, L. M., Liu, Y. & Yellen, G. Visual identification of individual transfected cells for electrophysiology using antibody-coated beads. BioTechniques 17, 876-881 (1994).
21. Heginbotham, L. & MacKinnon, R. The aromatic binding site for tetraethylammonium ion on potassium channels. Neuron 8, 483-491 (1992).
22. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches, Pflügers Arch. 391, 85-100 (1981).
23. Murrell-Lagnado, R. D. & Aldrich, R. W. Interactions of amino terminal domains of Shaker K channels with a pore blocking site studied with synthetic peptides. J. Gen. Physiol. 102, 949-975 (1993).
24. +-induced anomalous rectification in squid giant axons. J. Gen. Physiol. 50, 491-503 (1966).
25. Swenson, R. P. Jr Inactivation of potassium current in squid axon by a variety of quaternary ammonium ions. J. Gen. Physiol. 77, 235-271 (1981).
26. Gardner, S. & Thomton, J. Iditis: protein structure database. Acta Crystallogr. D Biol. Crystallogr. 54, 1071-1077 (1998).
27. Antz, C. et al. NMR structure of inactivation gates from mammalian voltage-dependent potassium channels. Nature 385, 272-275 (1997).