A universal, fully automated high throughput screening assay for pyrophosphate and phosphate release from enzymatic reactions

Combinatorial Chemistry and High Throughput Screening

Volumn 13, Issue 1, 2010, Pages 27-38

  Pegan, Scott D ^a   Tian, Yang ^a   Sershon, Valerie ^a   Mesecar, Andrew D ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Assay techniques; High throughput screening; Malachite green; Phosphate; Pyrophosphatase

Indexed keywords

ADENINE DERIVATIVE; BACTERIAL ENZYME; MALACHITE GREEN; NICOTINATE ADENINE DINUCLEOTIDE; NICOTINATE ADENINE MONONUCLEOTIDE; NICOTINATE MONONUCLEOTIDE ADENYLYLTRANSFERASE; NUCLEOTIDE DERIVATIVE; O SUCCINYLBENZOYL COENZYME A SYNTHETASE; PHOSPHATE; PYROPHOSPHATE; SUCCINYL COENZYME A SYNTHETASE; TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS ANTHRACIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME ASSAY; ENZYME MECHANISM; EQUIPMENT DESIGN; HIGH THROUGHPUT SCREENING; IC 50; LABORATORY AUTOMATION; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; ROBOTICS; SCALE UP; SPECTROMETRY;

ANIMALS; BACILLUS ANTHRACIS; DIPHOSPHATES; HIGH-THROUGHPUT SCREENING ASSAYS; PHOSPHATES; PYROPHOSPHATASES; ROSANILINE DYES; SACCHAROMYCES CEREVISIAE; SUCCINATE-COA LIGASES;

BACILLUS ANTHRACIS;

EID: 77949847432     PISSN: 13862073     EISSN: None     Source Type: Journal    
DOI: 10.2174/138620710790218203     Document Type: Article

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