A colorimetric assay for the determination of 4-diphosphocytidyl-2-C- methyl-D-erythritol 4-phosphate synthase activity

Analytical Biochemistry

Volumn 337, Issue 1, 2005, Pages 55-61

  Bernal, Cristobal ^a   Palacin, Celia ^b   Boronat, Albert ^a   Imperial, Santiago ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

^b SA Juan S

Author keywords

2 C Methyl D erythritol 4 phosphate pathway; 4 Diphosphocytidyl 2 C methyl D erythritol 4 phosphate synthase; Colorimetric assay; Isoprenoid biosynthesis; Malachite green; Pyrophosphatase

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CARBONATE MINERALS; COLORIMETRY; ENZYMES; LIPIDS;

2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE PATHWAYS; COLORIMETRIC ASSAYS; ISOPRENOID BIOSYNTHESIS; MALACHITE GREEN; PYROPHOSPHATASES; SYNTHASES;

DYES;

4 DIPHOSPHOCYTIDYL 2 C METHYL ERYTHRITOL 4 PHOSPHATE SYNTHASE; DYE; ISOPRENOID; MALACHITE GREEN; METHYL GROUP; PHOSPHATE; PHOSPHORUS DERIVATIVE; PYROPHOSPHATE; SYNTHETASE; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BIOLISTIC TRANSFORMATION; BIOSYNTHESIS; CATALYSIS; CATALYST; COLORIMETRY; CONTROLLED STUDY; ENERGY YIELD; ENZYME ACTIVITY; ENZYME INHIBITION; LABORATORY AUTOMATION; MOLECULAR BIOLOGY; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SCREENING TEST; SENSITIVITY ANALYSIS; SPECTROPHOTOMETRY; TECHNIQUE;

SIPROETA STELENES;

EID: 11844301664     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.10.011     Document Type: Article

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