Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Gunnarsen, Kristin S ^a   Lunde, Elin ^a   Kristiansen, Per E ^a   Bogen, Bjarne ^a,b   Sandlie, Inger ^a   Løset, Geir T ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL EXPRESSION SYSTEMS; BACTERIAL SYSTEMS; BIOPHYSICAL CHARACTERIZATION; CULTURE CONDITIONS; DISULPHIDE BONDS; DOMAIN ORIENTATION; DOWNSTREAM-PROCESSING; ELEVATED LEVEL; EXPRESSION SYSTEM; FOLDED PROTEINS; GENERAL EXPRESSION; GROWTH MEDIUM; HIGH THROUGHPUT SCREENING; HIGH YIELD; INCLUSION BODIES; LOW-YIELD; MOLECULAR DESIGN; MOLECULAR ENGINEERING; OVER-EXPRESSION; SINGLE CHAINS; T CELL RECEPTOR;

ANTIBODIES; BACTERIOLOGY; CELL CULTURE; CELL MEMBRANES; SUGAR (SUCROSE); THROUGHPUT;

MOLECULAR ORIENTATION;

CHAPERONE; PROTEIN FKPA; SUCROSE; T LYMPHOCYTE RECEPTOR; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; FKPA PROTEIN, E COLI; LYMPHOCYTE ANTIGEN RECEPTOR; MEMBRANE PROTEIN; PEPTIDYLPROLYL ISOMERASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; STRUCTURE ANALYSIS; BIOSYNTHESIS; CYTOPLASM; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM;

BACTERIA (MICROORGANISMS);

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; MEMBRANE PROTEINS; MOLECULAR CHAPERONES; PEPTIDYLPROLYL ISOMERASE; PERIPLASM; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, ANTIGEN, T-CELL;

EID: 77949542764     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-8     Document Type: Article

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